Most porcine oocytes used in studies on embryo biotechnology and the in vitro production of embryos are currently obtained from the ovaries of slaughtered gilts. The duration and temperature during ovary transportatio...Most porcine oocytes used in studies on embryo biotechnology and the in vitro production of embryos are currently obtained from the ovaries of slaughtered gilts. The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes. The effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes were examined in this study. Ovaries collected from a slaughterhouse were stored in vitro for 8 h under different temperatures. The results showed that more culturable COCs were isolated from the ovares stored at 39℃ than that from ovaries stored at 31℃ or 20℃ and before storage. Thirty-one centidegree was the best storage temperature in terms of cumulus expansion, nuclear maturation and morphology of the first polar body after in vitro maturation culture. The ability of cumulus expansion was completely lost in COCs derived from ovaries stored at 39℃for 8 hours. Ovary storage (at both 31℃ and at 20℃) increased the proportion of oocytes with the GVc configuration in which chromatin condensed into a single big clump at the nucleolus and the functional significance of this configuration needs further investigations.展开更多
目的探讨清开灵注射液对体外保存血小板功能的影响。方法取15(人)份机采血小板(≥250 mL/份),添加终浓度为1%清开灵注射液约1.25 mL/份,作为实验组22℃振荡保存,于保存d1、d3、d5、d8、d10和d14抽取标本,检测血小板的活化指标血栓弹力图...目的探讨清开灵注射液对体外保存血小板功能的影响。方法取15(人)份机采血小板(≥250 mL/份),添加终浓度为1%清开灵注射液约1.25 mL/份,作为实验组22℃振荡保存,于保存d1、d3、d5、d8、d10和d14抽取标本,检测血小板的活化指标血栓弹力图(TEG)﹑CD62p表达率、凋亡指标线粒体膜电位(JC-1);同时设做对照组:取与实验组等量(份)机采血小板、同保存条件,于保存d1、d3和d5抽取标本做相同项目的检测。比较2组各检测指标的差异。结果1)实验组保存d1~d14 TEG的K、α值在组内无明显变化(P>0.05);R值(min)由6.12±1.58增至11.02±2.26、CI值由0.27±1.24增至-10.47±3.51(P<0.05);MA值(mm)保存至d5时组内(间)尚无明显改变(P>0.05),至d8始降至53.18±2.71、至d14为22.88±3.45(P<0.05)。对照组保存d1~d5 TEG各指标,K值、A角无明显改变(P>0.05),R时明显延长,MA距减小,CI(绝对)值明显增加(P<0.05)。2)CD62p表达率(%):保存d5时实验组与对照组为70.50±9.12 vs 83.16±5.33(P<0.05),d14时为82.77±7.17(P<0.05)。3)JC-1(%):2组内血小板在常规保存期内无明显改变(P>0.05),只是d5时实验组与对照组为86.75±3.88 vs 70.36±19.8(P<0.05);实验组d8、d10、14d时分别为81.04±9.50 vs 71.38±8.77 vs 82.77±7.17(P<0.05)。结论添加1%清开灵注射液的机采血小板体外保存至14 d时的血小板活化、聚集及抗凋亡功能,与常规保存血小板相近。展开更多
文摘Most porcine oocytes used in studies on embryo biotechnology and the in vitro production of embryos are currently obtained from the ovaries of slaughtered gilts. The duration and temperature during ovary transportation and handling might, therefore, affect the recovery of culturable COCs, chromatin configuration and developmental competence of oocytes. The effects of ovary storage temperature on chromatin configuration and in vitro maturation of porcine oocytes were examined in this study. Ovaries collected from a slaughterhouse were stored in vitro for 8 h under different temperatures. The results showed that more culturable COCs were isolated from the ovares stored at 39℃ than that from ovaries stored at 31℃ or 20℃ and before storage. Thirty-one centidegree was the best storage temperature in terms of cumulus expansion, nuclear maturation and morphology of the first polar body after in vitro maturation culture. The ability of cumulus expansion was completely lost in COCs derived from ovaries stored at 39℃for 8 hours. Ovary storage (at both 31℃ and at 20℃) increased the proportion of oocytes with the GVc configuration in which chromatin condensed into a single big clump at the nucleolus and the functional significance of this configuration needs further investigations.
文摘目的探讨清开灵注射液对体外保存血小板功能的影响。方法取15(人)份机采血小板(≥250 mL/份),添加终浓度为1%清开灵注射液约1.25 mL/份,作为实验组22℃振荡保存,于保存d1、d3、d5、d8、d10和d14抽取标本,检测血小板的活化指标血栓弹力图(TEG)﹑CD62p表达率、凋亡指标线粒体膜电位(JC-1);同时设做对照组:取与实验组等量(份)机采血小板、同保存条件,于保存d1、d3和d5抽取标本做相同项目的检测。比较2组各检测指标的差异。结果1)实验组保存d1~d14 TEG的K、α值在组内无明显变化(P>0.05);R值(min)由6.12±1.58增至11.02±2.26、CI值由0.27±1.24增至-10.47±3.51(P<0.05);MA值(mm)保存至d5时组内(间)尚无明显改变(P>0.05),至d8始降至53.18±2.71、至d14为22.88±3.45(P<0.05)。对照组保存d1~d5 TEG各指标,K值、A角无明显改变(P>0.05),R时明显延长,MA距减小,CI(绝对)值明显增加(P<0.05)。2)CD62p表达率(%):保存d5时实验组与对照组为70.50±9.12 vs 83.16±5.33(P<0.05),d14时为82.77±7.17(P<0.05)。3)JC-1(%):2组内血小板在常规保存期内无明显改变(P>0.05),只是d5时实验组与对照组为86.75±3.88 vs 70.36±19.8(P<0.05);实验组d8、d10、14d时分别为81.04±9.50 vs 71.38±8.77 vs 82.77±7.17(P<0.05)。结论添加1%清开灵注射液的机采血小板体外保存至14 d时的血小板活化、聚集及抗凋亡功能,与常规保存血小板相近。
