AIM:To report the results of a medical management of sphincter of oddi dysfunction(SOD) after an intermediate follow-up period.METHODS:A total of 59 patients with SOD(2 men and 57 women,mean age 51 years old) were inc...AIM:To report the results of a medical management of sphincter of oddi dysfunction(SOD) after an intermediate follow-up period.METHODS:A total of 59 patients with SOD(2 men and 57 women,mean age 51 years old) were included in this prospective study.After medical treatment for one year,the patients were clinically re-evaluated after an average period of 30 mo.RESULTS:The distribution of the patients according to the Milwaukee's classification was the following:11 patients were type 1,34 were type 2 and 14 were type 3.Fourteen patients underwent an endoscopic sphincterotomy(ES) after one year of medical treatment.The median intermediate follow-up period was 29.8 ± 3 mo(3-72 mo).The initial effectiveness of the medical treatment was complete,partial and poor among 50.8%,13.5% and 35%,respectively,of the patients.At the end of the follow-up period,37 patients(62.7%) showed more than 50% improvement.The rate of improvement in patients who required ES was not significantly different compared with the patients treated conservatively(64.2% vs 62.2%,respectively).CONCLUSION:Our study confirms that conservative medical treatment could be an alternative to endoscopic sphincterotomy because,after an intermediate follow-up period,the two treatments show the same success rates.展开更多
Fabaceae is the third largest family of flowering plants and is unique among crops in their ability of fixing atmospheric nitrogen. Fabaceae is one of the few plant families with extensive genomic data available in mu...Fabaceae is the third largest family of flowering plants and is unique among crops in their ability of fixing atmospheric nitrogen. Fabaceae is one of the few plant families with extensive genomic data available in multiple species. The unprecedented complexity and impending completeness of these data create opportunities for discovering new approaches. The Legume and Medicago share much-conserved colinearity between their genomes which can be exploited for the genomic research in Leguminosae crops. In this study, 1,952,191 ESTs of 8 Leguminosae species were clustered into unigenes contigs and compared with Medicago truncatula gene indices. Almost all the unigenes of Leguminosae species showed high similarity with Medicago genes, except for those of Lens culinaris, where 95% of unigenes were found similar. A total of 10,874 SSRs were identified in the unigenes. Functional annotation of unigenes showed that the majority of the genes are present in metabolism and energy functional classes. It is expected that comparative genomic analysis between Medicago and related crop species will expedite research in other Legume species. This would be helpful for genomics as well as evolutionary studies, and the DNA markers developed can be used for mapping, tagging and cloning of specific important genes in Leguminosae.展开更多
Acute pancreatitis is one of the most common diseases in the everyday's practise of gastroenterologists and surgeons. However, the physicians' therapeutic armamentarium is very limited. The present letter to t...Acute pancreatitis is one of the most common diseases in the everyday's practise of gastroenterologists and surgeons. However, the physicians' therapeutic armamentarium is very limited. The present letter to the editor briefly describes the recent evidence from the literature with the aim to optimize a conservative management of patients with acute pancreatitis.展开更多
Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplif...Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.展开更多
AIM： To study the clinical outcomes of medical therapy in patients with right colonic diverticulitis. METHODS： The records of 189 patients with right colonic diverticulitis which was finally diagnosed by computed to...AIM： To study the clinical outcomes of medical therapy in patients with right colonic diverticulitis. METHODS： The records of 189 patients with right colonic diverticulitis which was finally diagnosed by computed tomography, ultrasonography, or operative findings were retrospectively reviewed. RESULTS： Of the 189 patients hospitalized for right colonic diverticulitis, the stages of diverticulitis by a modified Hinchey classification were 26 patients （13.8%） in stage 0, 139 patients （73.5%） in stage I a, 23 patients （12.2%） in stage I b, and 1 patient （0.5%） in stage Ⅲ. Medical therapy was undertaken in 185 of 189 patients （97.9%）. One hundred and eighty three of 185 patients were successfully treated with bowel rest and antibiotics. Two patients in stage I b required a resection or surgical drainage because of an inadequate response to conservative treatment. Recurrent diverticulitis developed in 15 of 183 patients （8.2%） who responded to medical therapy. All 15 patients who suffered a second attack had uncomplicated diverticulitis, and were successfully treated with medical therapy.CONCLUSION： Our results indicate that right colonic diverticulitis is essentially benign and image-guided conservative treatment is primarily required.展开更多
In this paper,it is shown that the homoclinic orbits exist in iterated functional systems.so do the solitary wave structures.Moreover,Harr father wavelet,Mexican Cap wavelet,and other closed form wavelets have this so...In this paper,it is shown that the homoclinic orbits exist in iterated functional systems.so do the solitary wave structures.Moreover,Harr father wavelet,Mexican Cap wavelet,and other closed form wavelets have this solitary wave structure,too So wavelet is a certain kind of solitary wave.展开更多
Mammalian genomes contain tens of thousands of long non-coding RNAs(lnc RNAs) that have been implicated in diverse biological processes. However, the lnc RNA transcriptomes of most mammalian species have not been esta...Mammalian genomes contain tens of thousands of long non-coding RNAs(lnc RNAs) that have been implicated in diverse biological processes. However, the lnc RNA transcriptomes of most mammalian species have not been established, limiting the evolutionary annotation of these novel transcripts. Based on RNA sequencing data from six tissues of nine species, we built comprehensive lnc RNA catalogs(4,142–42,558 lnc RNAs) covering the major mammalian species. Compared to protein-coding RNAs, expression of lnc RNAs exhibits striking lineage specificity. Notably, although 30%–99% human lnc RNAs are conserved across different species on DNA locus level, only 20%–27% of these conserved lnc RNA loci are detected to transcription, which represents a stark contrast to the proportion of conserved protein-coding genes(48%–80%). This finding provides a valuable resource for experimental scientists to study the mechanisms of lnc RNAs. Moreover, we constructed lnc RNA expression phylogenetic trees across nine mammals and demonstrated that lnc RNA expression profiles can reliably determine phylogenic placement in a manner similar to their coding counterparts. Our data also reveal that the evolutionary rate of lnc RNA expression varies among tissues and is significantly higher than those for protein-coding genes. To streamline the processes of browsing lnc RNAs and detecting their evolutionary statuses, we integrate all the data produced in this study into a database named Phylo NONCODE(http://www.bioinfo.org/phylo Noncode). Our work starts to place mammalian lnc RNAs in an evolutionary context and represent a rich resource for comparative and functional analyses of this critical layer of genome.展开更多
文摘AIM:To report the results of a medical management of sphincter of oddi dysfunction(SOD) after an intermediate follow-up period.METHODS:A total of 59 patients with SOD(2 men and 57 women,mean age 51 years old) were included in this prospective study.After medical treatment for one year,the patients were clinically re-evaluated after an average period of 30 mo.RESULTS:The distribution of the patients according to the Milwaukee's classification was the following:11 patients were type 1,34 were type 2 and 14 were type 3.Fourteen patients underwent an endoscopic sphincterotomy(ES) after one year of medical treatment.The median intermediate follow-up period was 29.8 ± 3 mo(3-72 mo).The initial effectiveness of the medical treatment was complete,partial and poor among 50.8%,13.5% and 35%,respectively,of the patients.At the end of the follow-up period,37 patients(62.7%) showed more than 50% improvement.The rate of improvement in patients who required ES was not significantly different compared with the patients treated conservatively(64.2% vs 62.2%,respectively).CONCLUSION:Our study confirms that conservative medical treatment could be an alternative to endoscopic sphincterotomy because,after an intermediate follow-up period,the two treatments show the same success rates.
文摘Fabaceae is the third largest family of flowering plants and is unique among crops in their ability of fixing atmospheric nitrogen. Fabaceae is one of the few plant families with extensive genomic data available in multiple species. The unprecedented complexity and impending completeness of these data create opportunities for discovering new approaches. The Legume and Medicago share much-conserved colinearity between their genomes which can be exploited for the genomic research in Leguminosae crops. In this study, 1,952,191 ESTs of 8 Leguminosae species were clustered into unigenes contigs and compared with Medicago truncatula gene indices. Almost all the unigenes of Leguminosae species showed high similarity with Medicago genes, except for those of Lens culinaris, where 95% of unigenes were found similar. A total of 10,874 SSRs were identified in the unigenes. Functional annotation of unigenes showed that the majority of the genes are present in metabolism and energy functional classes. It is expected that comparative genomic analysis between Medicago and related crop species will expedite research in other Legume species. This would be helpful for genomics as well as evolutionary studies, and the DNA markers developed can be used for mapping, tagging and cloning of specific important genes in Leguminosae.
文摘Acute pancreatitis is one of the most common diseases in the everyday's practise of gastroenterologists and surgeons. However, the physicians' therapeutic armamentarium is very limited. The present letter to the editor briefly describes the recent evidence from the literature with the aim to optimize a conservative management of patients with acute pancreatitis.
基金Supported by the National Natural Science Foundation of China (30801254)
文摘Objective To investigate the variability of human cytomegalovirus （HCMV） UL138 open reading frame （ORF） in clinical strains. Methods HCMV UL138 ORF was amplified by polymerase chain reaction （PCR） and PCR amplification products were sequenced directly, and the data were analyzed in 19 clinical strains. Results LIL138 ORF in all 30 clinical strains was amplified successfully. Compared with that of Toledo strain, the nucleotide and amino acid sequence identifies of LIL138 ORF in all strains were 97.41% to 99.41% and 98.24% to 99.42%, respectively. All of the nucleofide mutations were substitutions. The spatial structure and post-translational modification sites of HL138 encoded proteins were conserved. The result of phylogenetic tree showed that HCMV HL138 sequence variations were not definitely related with different clinical symptoms. Conclusion HCMV UL138 ORF in clinical strains is high conservation, which might be helpful for UL138 encoded protein to play a role in latent infection of HCMV.
基金Supported by The research paper scholarship of the graduate school of Kyung Hee University in the second semester of 2007
文摘AIM： To study the clinical outcomes of medical therapy in patients with right colonic diverticulitis. METHODS： The records of 189 patients with right colonic diverticulitis which was finally diagnosed by computed tomography, ultrasonography, or operative findings were retrospectively reviewed. RESULTS： Of the 189 patients hospitalized for right colonic diverticulitis, the stages of diverticulitis by a modified Hinchey classification were 26 patients （13.8%） in stage 0, 139 patients （73.5%） in stage I a, 23 patients （12.2%） in stage I b, and 1 patient （0.5%） in stage Ⅲ. Medical therapy was undertaken in 185 of 189 patients （97.9%）. One hundred and eighty three of 185 patients were successfully treated with bowel rest and antibiotics. Two patients in stage I b required a resection or surgical drainage because of an inadequate response to conservative treatment. Recurrent diverticulitis developed in 15 of 183 patients （8.2%） who responded to medical therapy. All 15 patients who suffered a second attack had uncomplicated diverticulitis, and were successfully treated with medical therapy.CONCLUSION： Our results indicate that right colonic diverticulitis is essentially benign and image-guided conservative treatment is primarily required.
基金The project supported by National Natural Science Foundation of China under Grant Nos.40045016 and No.40175016
文摘In this paper,it is shown that the homoclinic orbits exist in iterated functional systems.so do the solitary wave structures.Moreover,Harr father wavelet,Mexican Cap wavelet,and other closed form wavelets have this solitary wave structure,too So wavelet is a certain kind of solitary wave.
基金supported by Training Program of the Major Research Plan of the National Natural Science Foundation of China(91229120)
文摘Mammalian genomes contain tens of thousands of long non-coding RNAs(lnc RNAs) that have been implicated in diverse biological processes. However, the lnc RNA transcriptomes of most mammalian species have not been established, limiting the evolutionary annotation of these novel transcripts. Based on RNA sequencing data from six tissues of nine species, we built comprehensive lnc RNA catalogs(4,142–42,558 lnc RNAs) covering the major mammalian species. Compared to protein-coding RNAs, expression of lnc RNAs exhibits striking lineage specificity. Notably, although 30%–99% human lnc RNAs are conserved across different species on DNA locus level, only 20%–27% of these conserved lnc RNA loci are detected to transcription, which represents a stark contrast to the proportion of conserved protein-coding genes(48%–80%). This finding provides a valuable resource for experimental scientists to study the mechanisms of lnc RNAs. Moreover, we constructed lnc RNA expression phylogenetic trees across nine mammals and demonstrated that lnc RNA expression profiles can reliably determine phylogenic placement in a manner similar to their coding counterparts. Our data also reveal that the evolutionary rate of lnc RNA expression varies among tissues and is significantly higher than those for protein-coding genes. To streamline the processes of browsing lnc RNAs and detecting their evolutionary statuses, we integrate all the data produced in this study into a database named Phylo NONCODE(http://www.bioinfo.org/phylo Noncode). Our work starts to place mammalian lnc RNAs in an evolutionary context and represent a rich resource for comparative and functional analyses of this critical layer of genome.
