Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic n...Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oilcontaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16 S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.展开更多
The adoption of both Nature-based Solutions(NbS)and a system perspective is a hot issue and an area in which there is much room for improvement in ecological protection and restoration.The study takes Altay region as ...The adoption of both Nature-based Solutions(NbS)and a system perspective is a hot issue and an area in which there is much room for improvement in ecological protection and restoration.The study takes Altay region as an example,an area located in Xinjiang,China,which is an important ecological corridor of the national key functional area and the core area of the Belt and Road but has a fragile ecological environment and needs much enhancement in ecological protection and restoration.Guided by the concept of"mountains-rivers-forestsfarmlands-lakes-grasslands(MRFFLG)system is a life community",the coupling relationship of the various MRFFLG elements,the main ecological environmental problems and the integrated countermeasures for protection and restoration in Altay region have been analyzed in this study.The study shows that Altay region has a typical meta-ecosystem of mountain,oasis,and desert,and the connections by rivers and lakes make the ecosystem links within the region much closer.The interweaving of resource-environmental-ecological issues of the aquatic ecosystem,degradation of the quality of terrestrial ecosystems such as forests,grasslands and farmlands,the increased ecosystem fragmentation and ecological risks are the major ecological environmental issues in this region.This study takes the improvement of the ecosystem health and function in Altay region as the core goal of regional ecological protection and restoration.First,we establish a zoning governance pattern from the perspective of ecosystem integrity;second,we design governance strategies for different zones with the goal of synergistic improvements in the ecosystem functions;and finally,we clarify the key engineering tasks in different zones from the perspective of ecosystem services.Ecological conservation and restoration of the MRFFLG system is a prime example of the application and development of NbS in China.This paper constructs an analytical framework for identifying regional life communities,diagnosing ecological protection and restoration of zoning problems,and proposing classified solutions for ecological protection tasks for the protection of the MRFFLG system.This research will enrich the theory and technology for the protection and restoration of MRFFLG systems,and provide guidance for the practice of ecological protection and restoration in Altay region.展开更多
基金Supported by the Hundred Talents Program of the Chinese Academy of Sciences awarded to Dr.Xiaoke HUthe Key Research Program of Chinese Academy of Sciences(No.KZZD-EW-14)+1 种基金the Science and Technology Program of Shandong Province(No.2013GHY11534)the National Natural Science Foundation of China(Nos.41376138,41576165)
文摘Bioremediation, is an effective and environment-friendly method of cleaning up crude oil pollution after an oil spill. However, the in situ bioremediation of crude oil is usually inhibited by deficiency of inorganic nutrients. To understand the effects of nutrient addition on the bioremediation of crude oil and the succession of bacterial communities during process of bioremediation, microcosms containing oilcontaminated sediments were constructed and biodegradation of crude oil was assessed based on the depletion of different ingredients. We used two culture-independent methods, denaturing gradient gel electrophoresis and a 16 S rRNA gene based clone library, to analyze the succession of bacterial communities. The results suggested n-alkanes were degraded after 30 days and that nutrient amendments significantly improved the efficiency of their biodegradation. Moreover, oil contamination and nutrient amendments could dramatically change bacterial community structures. Lower diversity was detected after being contaminated with oil. For instance, bacterial clones affiliated with the phylum Armatimonadetes, Firmicutes, Gemmatimonadetes, and Planctomycetes and the class Deltaproteobacteria and Epsilonproteobacteria could not be identified after 30 days of incubation with crude oil. However, "professional hydrocarbonocastic bacteria" became abundant in samples treated with oil during the bioremediation period, while these clones were almost completely absent from the control plots. Interestingly, bioinformatics analysis showed that even when dramatic differences in oil biodegradation efficiency were observed, bacterial communities in the plots with nutrient amendments were not significantly different from those in plots treated with oil alone. These findings indicated that nutrient amendments could stimulate the process of biodegradation but had less impact on bacterial communities. Overall, nutrient amendments might be able to stimulate the growth of n-alkane degrading bacteria.
基金The National Natural Science Foundation of China(41701601,41871196)The Scientific Research Project in Altay Region,Xinjiang Uygur Autonomous Region of China(2019-529)。
文摘The adoption of both Nature-based Solutions(NbS)and a system perspective is a hot issue and an area in which there is much room for improvement in ecological protection and restoration.The study takes Altay region as an example,an area located in Xinjiang,China,which is an important ecological corridor of the national key functional area and the core area of the Belt and Road but has a fragile ecological environment and needs much enhancement in ecological protection and restoration.Guided by the concept of"mountains-rivers-forestsfarmlands-lakes-grasslands(MRFFLG)system is a life community",the coupling relationship of the various MRFFLG elements,the main ecological environmental problems and the integrated countermeasures for protection and restoration in Altay region have been analyzed in this study.The study shows that Altay region has a typical meta-ecosystem of mountain,oasis,and desert,and the connections by rivers and lakes make the ecosystem links within the region much closer.The interweaving of resource-environmental-ecological issues of the aquatic ecosystem,degradation of the quality of terrestrial ecosystems such as forests,grasslands and farmlands,the increased ecosystem fragmentation and ecological risks are the major ecological environmental issues in this region.This study takes the improvement of the ecosystem health and function in Altay region as the core goal of regional ecological protection and restoration.First,we establish a zoning governance pattern from the perspective of ecosystem integrity;second,we design governance strategies for different zones with the goal of synergistic improvements in the ecosystem functions;and finally,we clarify the key engineering tasks in different zones from the perspective of ecosystem services.Ecological conservation and restoration of the MRFFLG system is a prime example of the application and development of NbS in China.This paper constructs an analytical framework for identifying regional life communities,diagnosing ecological protection and restoration of zoning problems,and proposing classified solutions for ecological protection tasks for the protection of the MRFFLG system.This research will enrich the theory and technology for the protection and restoration of MRFFLG systems,and provide guidance for the practice of ecological protection and restoration in Altay region.
