目的探究在紫外线暴露下环指蛋白20(ring finger protein 20,RNF20)低表达对胃癌细胞DNA损伤修复的影响及其相关作用机制。方法实验采用慢病毒载体构建稳定低表达胃癌细胞系,分为对照组和RNF20敲低组,用CCK-8法检测两组细胞的增殖情况,...目的探究在紫外线暴露下环指蛋白20(ring finger protein 20,RNF20)低表达对胃癌细胞DNA损伤修复的影响及其相关作用机制。方法实验采用慢病毒载体构建稳定低表达胃癌细胞系,分为对照组和RNF20敲低组,用CCK-8法检测两组细胞的增殖情况,用总共照射剂量为20 J/m^(2)紫外线照射胃癌MGC803细胞,采用Western blotting及免疫荧光技术检测两组细胞γ-H2AX、RAD51和p21的情况。结果荧光显微镜观察两组细胞均有绿色荧光蛋白表达;CCK-8显示RNF20表达降低会促进胃癌细胞增殖;敲低组细胞中RNF20蛋白较对照组表达降低。与对照组相比,经20 J/m^(2)紫外线照射细胞后,敲低组γ-H2AX消失更加迟缓,RAD51蛋白表达降低,p21蛋白表达下降趋势更慢。结论RNF20敲低会抑制紫外线诱导的胃癌细胞DNA损伤修复过程。展开更多
髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病...髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病率逐渐上升,髋臼盂唇治疗的需求得到显著的上升。随着对关节镜的运用成熟,髋关节镜技术已经成为治疗髋臼盂唇损伤的主要手术形式,包括:盂唇清理术、修复术、重建术、加强术。其主要作用都是恢复盂唇的密闭性及缓解应力分布,从而恢复髋臼功能性。因此本文主要就髋臼盂唇损伤修复进行综述。Background: The acetabular labrum is located in the hip joint and is a fibrocartilaginous ring attached to the bony acetabular rim, which is an important structure for maintaining the function of the hip joint. Damage to the labrum will change the biomechanical mechanism of the hip joint, causing joint instability and ultimately leading to the development of osteoarthritis. Due to the development of imaging technology and the popularity of sports among all people, the incidence of acetabular labral injury is gradually increasing among young people, and the demand for acetabular labral treatment has risen significantly. As the use of arthroscopy has matured, hip arthroscopy has become the main surgical form of treatment for acetabular labral injuries, including labral debridement, repair, reconstruction, and strengthening. The main purpose of these procedures is to restore labral confinement and relieve stress distribution, thus restoring acetabular function. Therefore, this article mainly reviews the repair of acetabular labral injury.展开更多
目的 探究关节镜下肩袖修复术与小切口肩袖损伤修复术治疗结果对比研究;方法 择肩袖损伤患者116例为研究对象,均由2020年5月至2023年5月期间本院收治的患者,依据手术方式差异将患者分为观察组58例和对照组58例,分别采用关节镜下肩袖修...目的 探究关节镜下肩袖修复术与小切口肩袖损伤修复术治疗结果对比研究;方法 择肩袖损伤患者116例为研究对象,均由2020年5月至2023年5月期间本院收治的患者,依据手术方式差异将患者分为观察组58例和对照组58例,分别采用关节镜下肩袖修复术和小切口肩袖损伤修复术治疗,对比两组患者治疗效果;结果 干预治疗后,与对照组比,观察组患者白细胞介素-8(Interleukin-8,IL-8)、C反应蛋白(C-reactive protein,CRP)和肿瘤坏死因子-α (Tumor Necrosis Factor-α,TNF-α)血液炎症因子水平明显偏低、美国肩肘外科协会(American Shoulder and Elbow Surgeons,ASES)评分和美国加州大学肩关节(the University of California atLos Angeles shoulder rating scale,U-CLA)评分明显偏高、视觉模拟评分法(Visual analogue scale,VAS)明显偏低(P<0.05)。观察组58例患者总体有效人数53例,占比91.4%,明显高于对照组(χ^(2)=6.042,P=0.014<0.05)。结论 关节镜下肩袖修复术在肩袖损伤患者治疗中临床治疗效果佳,术后关节功能恢复好,炎症反应低。展开更多
2024年6月6日,北京大学基础医学院王嘉东课题组在PNAS期刊上发表文章“The ARK2N-CK2 complex initiates transcription-coupled repair through enhancing the interaction ofCSB with lesion-stalled RNAPⅡ”(ARK2N-CK2复合体通过增...2024年6月6日,北京大学基础医学院王嘉东课题组在PNAS期刊上发表文章“The ARK2N-CK2 complex initiates transcription-coupled repair through enhancing the interaction ofCSB with lesion-stalled RNAPⅡ”(ARK2N-CK2复合体通过增强CSB与被损伤阻滞的RNAPⅡ的结合启动转录偶联修复),揭示了ARK2N-CK2复合体参与光辐射损伤修复的新机制。展开更多
文摘髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病率逐渐上升,髋臼盂唇治疗的需求得到显著的上升。随着对关节镜的运用成熟,髋关节镜技术已经成为治疗髋臼盂唇损伤的主要手术形式,包括:盂唇清理术、修复术、重建术、加强术。其主要作用都是恢复盂唇的密闭性及缓解应力分布,从而恢复髋臼功能性。因此本文主要就髋臼盂唇损伤修复进行综述。Background: The acetabular labrum is located in the hip joint and is a fibrocartilaginous ring attached to the bony acetabular rim, which is an important structure for maintaining the function of the hip joint. Damage to the labrum will change the biomechanical mechanism of the hip joint, causing joint instability and ultimately leading to the development of osteoarthritis. Due to the development of imaging technology and the popularity of sports among all people, the incidence of acetabular labral injury is gradually increasing among young people, and the demand for acetabular labral treatment has risen significantly. As the use of arthroscopy has matured, hip arthroscopy has become the main surgical form of treatment for acetabular labral injuries, including labral debridement, repair, reconstruction, and strengthening. The main purpose of these procedures is to restore labral confinement and relieve stress distribution, thus restoring acetabular function. Therefore, this article mainly reviews the repair of acetabular labral injury.
文摘目的 探究关节镜下肩袖修复术与小切口肩袖损伤修复术治疗结果对比研究;方法 择肩袖损伤患者116例为研究对象,均由2020年5月至2023年5月期间本院收治的患者,依据手术方式差异将患者分为观察组58例和对照组58例,分别采用关节镜下肩袖修复术和小切口肩袖损伤修复术治疗,对比两组患者治疗效果;结果 干预治疗后,与对照组比,观察组患者白细胞介素-8(Interleukin-8,IL-8)、C反应蛋白(C-reactive protein,CRP)和肿瘤坏死因子-α (Tumor Necrosis Factor-α,TNF-α)血液炎症因子水平明显偏低、美国肩肘外科协会(American Shoulder and Elbow Surgeons,ASES)评分和美国加州大学肩关节(the University of California atLos Angeles shoulder rating scale,U-CLA)评分明显偏高、视觉模拟评分法(Visual analogue scale,VAS)明显偏低(P<0.05)。观察组58例患者总体有效人数53例,占比91.4%,明显高于对照组(χ^(2)=6.042,P=0.014<0.05)。结论 关节镜下肩袖修复术在肩袖损伤患者治疗中临床治疗效果佳,术后关节功能恢复好,炎症反应低。
文摘2024年6月6日,北京大学基础医学院王嘉东课题组在PNAS期刊上发表文章“The ARK2N-CK2 complex initiates transcription-coupled repair through enhancing the interaction ofCSB with lesion-stalled RNAPⅡ”(ARK2N-CK2复合体通过增强CSB与被损伤阻滞的RNAPⅡ的结合启动转录偶联修复),揭示了ARK2N-CK2复合体参与光辐射损伤修复的新机制。
