Batch production of bovine adult somatic cell cloned embryos by zona-free 2-half cytoplast manipulation technique resulted in the following rates(36 replicates): fusion 95 7% (3678/3842), cleavage 87 2% (3180/3645), b...Batch production of bovine adult somatic cell cloned embryos by zona-free 2-half cytoplast manipulation technique resulted in the following rates(36 replicates): fusion 95 7% (3678/3842), cleavage 87 2% (3180/3645), blastocyst 42 2%(1540/3645), freezable embryos 31 4%(1145/3645), 30 days pregnancy after transfer of vitrified/thawed embryos 28 1% (48/171) Five live calves were born at term from 19th of July until 26th of October 2003 Four of the calves were delivered by cesarean section, and three died within 1 h and another at 5 days after delivery The only calf delivered naturally is still alive and展开更多
文摘Batch production of bovine adult somatic cell cloned embryos by zona-free 2-half cytoplast manipulation technique resulted in the following rates(36 replicates): fusion 95 7% (3678/3842), cleavage 87 2% (3180/3645), blastocyst 42 2%(1540/3645), freezable embryos 31 4%(1145/3645), 30 days pregnancy after transfer of vitrified/thawed embryos 28 1% (48/171) Five live calves were born at term from 19th of July until 26th of October 2003 Four of the calves were delivered by cesarean section, and three died within 1 h and another at 5 days after delivery The only calf delivered naturally is still alive and
