【背景】内生菌几乎存在于所有目前已研究过的植物中,感染内生真菌的植物宿主往往具有生长快速、抗逆境、抗病害、抗天敌等优势。目前,植物内生菌多样性得到了广泛关注,然而现阶段对该领域进行定量统计、分析的研究报道较少。【目的】...【背景】内生菌几乎存在于所有目前已研究过的植物中,感染内生真菌的植物宿主往往具有生长快速、抗逆境、抗病害、抗天敌等优势。目前,植物内生菌多样性得到了广泛关注,然而现阶段对该领域进行定量统计、分析的研究报道较少。【目的】对植物内生真菌多样性领域文献运用计量学方法进行分析,探讨该领域的研究现状及研究热点。【方法】通过文献计量学方法,基于Web of Science(WOS)核心合集数据库,以“endophytic fungal diversity and plant”为检索词进行主题检索,统计1992–2022年间“植物内生真菌多样性”研究文献的刊文数量、期刊、研究机构、主要作者和关键词的分布情况,并利用CiteSpace软件进行可视化分析。【结果】通过关键词突现分析发现biodiversity(生物多样性)、symbiosis(共生)、soil(土壤)3个关键词与该领域文献发表量突增的时段密切相关。并通过关键词聚类分析,得到9个关键词标签,具体3个热点研究方向。【结论】“Endophytic fungal diversity and plant”研究的英文文献数量从2019年开始快速增加,内生真菌多样性、内生真菌促进宿主生长、内生真菌次生代谢产物药用性为现阶段的研究热点。未来应该加强该领域高影响力作者、机构的合作,针对本领域的3个热点及不足展开深入、系统的研究。展开更多
Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the e...Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants（Caralluma acutangula, Rhazya stricta, and Moringa peregrina） and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp.（6 isolates）, Alternaria sp.（2）, Bipolaris sp.（1）, and Cladosporium sp.（1） based on 18 S rD NA sequencing and phylogenetic analysis. The culture filtrates（CFs; 25%, 50%, and 100% concentrations） from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF（100%） of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry（GC/MS） analysis showed the highest indole acetic acid content（（54.31±0.21） μmol/L） in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.展开更多
文摘【背景】内生菌几乎存在于所有目前已研究过的植物中,感染内生真菌的植物宿主往往具有生长快速、抗逆境、抗病害、抗天敌等优势。目前,植物内生菌多样性得到了广泛关注,然而现阶段对该领域进行定量统计、分析的研究报道较少。【目的】对植物内生真菌多样性领域文献运用计量学方法进行分析,探讨该领域的研究现状及研究热点。【方法】通过文献计量学方法,基于Web of Science(WOS)核心合集数据库,以“endophytic fungal diversity and plant”为检索词进行主题检索,统计1992–2022年间“植物内生真菌多样性”研究文献的刊文数量、期刊、研究机构、主要作者和关键词的分布情况,并利用CiteSpace软件进行可视化分析。【结果】通过关键词突现分析发现biodiversity(生物多样性)、symbiosis(共生)、soil(土壤)3个关键词与该领域文献发表量突增的时段密切相关。并通过关键词聚类分析,得到9个关键词标签,具体3个热点研究方向。【结论】“Endophytic fungal diversity and plant”研究的英文文献数量从2019年开始快速增加,内生真菌多样性、内生真菌促进宿主生长、内生真菌次生代谢产物药用性为现阶段的研究热点。未来应该加强该领域高影响力作者、机构的合作,针对本领域的3个热点及不足展开深入、系统的研究。
基金Project supported by the Oman Research Council(FURAP Program)the Korea Institute of Planning and Evaluation for Technology in Food,Agriculture,Forestry and Fisheries(IPET) through Agriculture,Food and Rural Affairs Research Center Support Program,funded by Ministry of Agriculture,Food and Rural Affairs(MAFRA)(716001-7)
文摘Medicinal plants have been used by marginal communities to treat various ailments. However, the potential of endophytes within these bio-prospective medicinal plants remains unknown. The present study elucidates the endophytic diversity of medicinal plants（Caralluma acutangula, Rhazya stricta, and Moringa peregrina） and the endophyte role in seed growth and oxidative stress. Various organs of medicinal plants yielded ten endophytes, which were identified as Phoma sp.（6 isolates）, Alternaria sp.（2）, Bipolaris sp.（1）, and Cladosporium sp.（1） based on 18 S rD NA sequencing and phylogenetic analysis. The culture filtrates（CFs; 25%, 50%, and 100% concentrations） from these endophytes were tested against the growth of normal and dwarf mutant rice lines. Endophytic CF exhibited dose-dependent growth stimulation and suppression effects. CF（100%） of Phoma sp. significantly increased rice seed germination and growth compared to controls and other endophytes. This growth-promoting effect was due to the presence of indole acetic acid in endophytic CF. The gas chromatography/mass spectrometry（GC/MS） analysis showed the highest indole acetic acid content（（54.31±0.21） μmol/L） in Bipolaris sp. In addition, the isolate of Bipolaris sp. exhibited significantly higher radical scavenging and anti-lipid peroxidation activity than the other isolates. Bipolaris sp. and Phoma sp. also exhibited significantly higher flavonoid and phenolic contents. The medicinal plants exhibited the presence of bio-prospective endophytic strains, which could be used for the improvement of crop growth and the mitigation of oxidative stresses.
