目的:观察复方丹参注射液对体外培养猪内皮前体细胞氧化应激损伤的保护作用。方法:建立猪内皮前体细胞体外培养模型,在培养液中加入100μm o l/L过氧化氢及不同浓度(1、2、5m g/L)复方丹参注射液,测定细胞增殖活力(MTT法)、乳酸脱氢酶(L...目的:观察复方丹参注射液对体外培养猪内皮前体细胞氧化应激损伤的保护作用。方法:建立猪内皮前体细胞体外培养模型,在培养液中加入100μm o l/L过氧化氢及不同浓度(1、2、5m g/L)复方丹参注射液,测定细胞增殖活力(MTT法)、乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量及细胞凋亡率。结果:过氧化氢使细胞增殖活力下降,使LDH、MDA含量增加,细胞凋亡率上升,复方丹参注射液可使上述结果改变并呈浓度依赖性。结论:复方丹参注射液对内皮前体细胞氧化应激损伤有保护作用。展开更多
Endothelial progenitor cells (EPCs) exist in bone marrow, umbilical cord blood and peripheral blood of adult mammals, including humans. Furthermore, the discovery of EPCs has led to the notion of adult vasculogenesis,...Endothelial progenitor cells (EPCs) exist in bone marrow, umbilical cord blood and peripheral blood of adult mammals, including humans. Furthermore, the discovery of EPCs has led to the notion of adult vasculogenesis, in which bone marrow (BM)-derived EPCs home to and incorporate into sites of new blood vessel formation, where they differentiate into endothelial cells, which is consistent with postnatal vasculogenesis. It has become apparent that circulating BM-derived EPCs are involved in promoting physiologic and pathologic neovascularization, such as wound healing and tumor growth. They are of great clinical importance in pro- or anti-angiogenic therapies. [展开更多
文摘目的:观察复方丹参注射液对体外培养猪内皮前体细胞氧化应激损伤的保护作用。方法:建立猪内皮前体细胞体外培养模型,在培养液中加入100μm o l/L过氧化氢及不同浓度(1、2、5m g/L)复方丹参注射液,测定细胞增殖活力(MTT法)、乳酸脱氢酶(LDH)活性、丙二醛(MDA)含量及细胞凋亡率。结果:过氧化氢使细胞增殖活力下降,使LDH、MDA含量增加,细胞凋亡率上升,复方丹参注射液可使上述结果改变并呈浓度依赖性。结论:复方丹参注射液对内皮前体细胞氧化应激损伤有保护作用。
文摘Endothelial progenitor cells (EPCs) exist in bone marrow, umbilical cord blood and peripheral blood of adult mammals, including humans. Furthermore, the discovery of EPCs has led to the notion of adult vasculogenesis, in which bone marrow (BM)-derived EPCs home to and incorporate into sites of new blood vessel formation, where they differentiate into endothelial cells, which is consistent with postnatal vasculogenesis. It has become apparent that circulating BM-derived EPCs are involved in promoting physiologic and pathologic neovascularization, such as wound healing and tumor growth. They are of great clinical importance in pro- or anti-angiogenic therapies. [
