A series of pot experiments and field trials were carried out to evaluate the effects of arbuscular mycor- rhizal fungi （AMF） on activities of soil enzymes and carbon sequestration capacity in reclaimed mine soil. A...A series of pot experiments and field trials were carried out to evaluate the effects of arbuscular mycor- rhizal fungi （AMF） on activities of soil enzymes and carbon sequestration capacity in reclaimed mine soil. A complex substrate of coal gangue, fly ash and sludge was used as reclaimed mine soil, and ryegrass was planted with AMF inoculation to construct a plant-complex substrate-microbe ecological restoration sys- tem. The changes to the soil organic carbon （SOC）, activities of soil enzymes and glomalin-related soil protein （GRSP） were measured and the effects of AMF on activities of soil enzymes and carbon sequestra- tion capacity （n reclaimed mine soil were analyzed. The results show that the contents of GRSP （total glo- malin （TG） and easily extractable glomalin （EEG））, SOC and activities of enzymes increased, and the increments were higher in the AMF inoculation treated plant-complex substrate-microbe ecological res- toration systems than those with no AMF inoculated treatments after 12 months of ryegrass growth. TG, EEG and soil enzyme activity have a significant positive correlation, and the correlative coefficient was 0.427-0.573; SOC and TG, EEG have a significant positive correlation （p 〈 0.01 ）, indicating that AMF plays an important role in carbon sequestration of reclaimed mine soils.展开更多
Objective： Present study aimed at further comprehensive functional, histomorphometrical and immunohistochemical assessment of peripheral nerve regeneration using rat sciatic nerve transection model.Methods： The 10-m...Objective： Present study aimed at further comprehensive functional, histomorphometrical and immunohistochemical assessment of peripheral nerve regeneration using rat sciatic nerve transection model.Methods： The 10-mm rat sciatic nerve gap was created in rats. In control group nerve stumps were sutured to adjacent muscle and in treatment group the gap was bridged using an inside-out vein graft. In sham-operated group the nerve was manipulated and left intact. All animals underwent walking track analysis test 4, 8, and 12 weeks after surgery.Subsequently, muscle mass measurement was performed to assess reenervation, histological examination to observe the sciatic nerve regeneration morphologically and immunohistochemistry to detect Schwann cells using anti S-100. Results were analyzed using a factorial ANOVA with two between-subjects factors. Bonferroni test for pairwise comparisons was used to examine the effect of treatments.Results： Functional analysis ofmyelinated nerve fibers showed that nerve function improved significantly in the time course in treatment group. However, quantitative morphometrical analysis of myelinated nerve fibers showed that there was no significant difference between 8 and 12 weeks in treatment group. Muscle weight ratio was bigger and weight loss of the gastrocnemius muscle was ameliorated by inside-out vein grafting. The position of positive immunohistochemical reactions further implied that regenerated axons and Schwann cell-like cells existed after vein grafting was performed, and was accompanied by the process of myelination and structural recovery of regenerated nerves.Conclusion： Functional analysis of peripheral nerve repair is far more reliable than quantitative morphometrical analysis展开更多
基金supported by the Environmental Science andTechnology Fund of Environmental Protection Department of Jiangsu Province,China (No. 2007024)
文摘A series of pot experiments and field trials were carried out to evaluate the effects of arbuscular mycor- rhizal fungi （AMF） on activities of soil enzymes and carbon sequestration capacity in reclaimed mine soil. A complex substrate of coal gangue, fly ash and sludge was used as reclaimed mine soil, and ryegrass was planted with AMF inoculation to construct a plant-complex substrate-microbe ecological restoration sys- tem. The changes to the soil organic carbon （SOC）, activities of soil enzymes and glomalin-related soil protein （GRSP） were measured and the effects of AMF on activities of soil enzymes and carbon sequestra- tion capacity （n reclaimed mine soil were analyzed. The results show that the contents of GRSP （total glo- malin （TG） and easily extractable glomalin （EEG））, SOC and activities of enzymes increased, and the increments were higher in the AMF inoculation treated plant-complex substrate-microbe ecological res- toration systems than those with no AMF inoculated treatments after 12 months of ryegrass growth. TG, EEG and soil enzyme activity have a significant positive correlation, and the correlative coefficient was 0.427-0.573; SOC and TG, EEG have a significant positive correlation （p 〈 0.01 ）, indicating that AMF plays an important role in carbon sequestration of reclaimed mine soils.
文摘Objective： Present study aimed at further comprehensive functional, histomorphometrical and immunohistochemical assessment of peripheral nerve regeneration using rat sciatic nerve transection model.Methods： The 10-mm rat sciatic nerve gap was created in rats. In control group nerve stumps were sutured to adjacent muscle and in treatment group the gap was bridged using an inside-out vein graft. In sham-operated group the nerve was manipulated and left intact. All animals underwent walking track analysis test 4, 8, and 12 weeks after surgery.Subsequently, muscle mass measurement was performed to assess reenervation, histological examination to observe the sciatic nerve regeneration morphologically and immunohistochemistry to detect Schwann cells using anti S-100. Results were analyzed using a factorial ANOVA with two between-subjects factors. Bonferroni test for pairwise comparisons was used to examine the effect of treatments.Results： Functional analysis ofmyelinated nerve fibers showed that nerve function improved significantly in the time course in treatment group. However, quantitative morphometrical analysis of myelinated nerve fibers showed that there was no significant difference between 8 and 12 weeks in treatment group. Muscle weight ratio was bigger and weight loss of the gastrocnemius muscle was ameliorated by inside-out vein grafting. The position of positive immunohistochemical reactions further implied that regenerated axons and Schwann cell-like cells existed after vein grafting was performed, and was accompanied by the process of myelination and structural recovery of regenerated nerves.Conclusion： Functional analysis of peripheral nerve repair is far more reliable than quantitative morphometrical analysis
