据Bhattacharjee S 2024年2月1日[Cell,2024,187(3):782-796.e23.]报道,美国哥伦比亚大学研究人员开发了基于微流控芯片的时间分辨冷冻电子显微镜方法,并揭示了Hfl X介导的核糖体回收动态路径。所有生命过程都涉及细胞中分子之间的相互...据Bhattacharjee S 2024年2月1日[Cell,2024,187(3):782-796.e23.]报道,美国哥伦比亚大学研究人员开发了基于微流控芯片的时间分辨冷冻电子显微镜方法,并揭示了Hfl X介导的核糖体回收动态路径。所有生命过程都涉及细胞中分子之间的相互作用。例如,核糖体是一种由蛋白质和RNA组成的巨大分子机器,在将遗传信息翻译成蛋白质时与转运RNA(tRNA)和多种蛋白质因子相互作用。展开更多
2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电...2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电子显微镜(cryogenic electron microscopy,cryo-EM)将成为解密大分子结构的最强大工具。这些结构对于理解生化机制和药物开发至关重要,而更有效地解析它们的方法可以加快此类工作。展开更多
Protein could carry out its biological function only when it is folded into the right conformation,and the conformation might be changing during the activation process,therefore protein dynamical structure provides a ...Protein could carry out its biological function only when it is folded into the right conformation,and the conformation might be changing during the activation process,therefore protein dynamical structure provides a key clue to understand its function.Although many protein static structures have been resolved by X-ray crystallography,2D NM and cryoelectron microscope at atomic level,the static structure could be different from the dynamical structure,especially under physiological condition.In their continuous effort to study the protein dynamical structure with temperature-jump time-resolved infrared spectroscopy(Biophys.J.2007,93,2756–2766;Biophys.J.2009,97,2756–2766),a team lead by Prof.WENG Yuxiang from the Institute of Physics(IOP),Chinese Academy of Sciences recently collaborated with Prof.CHANG展开更多
In-situ characterization of non-aqueous nano-dispersion systems(NANDS) by freeze-etching transmission electron microscope(FETEM) was reported.To improve just-for-once successive rate of specimen preparation and get go...In-situ characterization of non-aqueous nano-dispersion systems(NANDS) by freeze-etching transmission electron microscope(FETEM) was reported.To improve just-for-once successive rate of specimen preparation and get good characterization results,an improving specimen preparation method of freezing etching was developed.Size,distribution and morphology of NANDS were directly visualized.Some information of particle dispersion feature and particle density can also be obtained.Reproductivity of the FETEM characterization is excellent.Comparing with laser scattering method,which is liable to give positive error especially for small size particle anchoring disperser,FETEM characterization can give more accurate measurement of particle size.Moreover,FETEM can give dispersion feature of nanoparticle in non-aqueous medium.展开更多
文摘据Bhattacharjee S 2024年2月1日[Cell,2024,187(3):782-796.e23.]报道,美国哥伦比亚大学研究人员开发了基于微流控芯片的时间分辨冷冻电子显微镜方法,并揭示了Hfl X介导的核糖体回收动态路径。所有生命过程都涉及细胞中分子之间的相互作用。例如,核糖体是一种由蛋白质和RNA组成的巨大分子机器,在将遗传信息翻译成蛋白质时与转运RNA(tRNA)和多种蛋白质因子相互作用。
文摘2020年1月21日,全球顶级杂志《自然》(Nature)发表综述文章,“Technologies to watch in 2020”,请七位行业思想领袖预测技术发展将在今年产生的重大影响。清华大学生命科学学院王宏伟教授:更好地冷冻电镜样品在两到三年中透射式冷冻电子显微镜(cryogenic electron microscopy,cryo-EM)将成为解密大分子结构的最强大工具。这些结构对于理解生化机制和药物开发至关重要,而更有效地解析它们的方法可以加快此类工作。
基金supported by the National Natural Science Foundation of China(Grant No.20925313 and 21090342)the National Basic Research Program of China(Grant No.2006CB910302)the Chinese Academy of Sciences Innovation Program(Grant No.KJCX2-YW-W25)
文摘Protein could carry out its biological function only when it is folded into the right conformation,and the conformation might be changing during the activation process,therefore protein dynamical structure provides a key clue to understand its function.Although many protein static structures have been resolved by X-ray crystallography,2D NM and cryoelectron microscope at atomic level,the static structure could be different from the dynamical structure,especially under physiological condition.In their continuous effort to study the protein dynamical structure with temperature-jump time-resolved infrared spectroscopy(Biophys.J.2007,93,2756–2766;Biophys.J.2009,97,2756–2766),a team lead by Prof.WENG Yuxiang from the Institute of Physics(IOP),Chinese Academy of Sciences recently collaborated with Prof.CHANG
基金supported by the National Natural Science Foundation of China(No.20953003)the Natural Science Foundation of Shandong Province of China(No.Z2008B08 and ZR2009BZ001)Taishan Scholar Foundation of Shandong Province of China(No.ts20070713).
基金Funded by National Natural Science Foundation of China(No.50572121) Key Pre-research Foundation of Weapon and Equipment(No. 9140A27010206JB35)
文摘In-situ characterization of non-aqueous nano-dispersion systems(NANDS) by freeze-etching transmission electron microscope(FETEM) was reported.To improve just-for-once successive rate of specimen preparation and get good characterization results,an improving specimen preparation method of freezing etching was developed.Size,distribution and morphology of NANDS were directly visualized.Some information of particle dispersion feature and particle density can also be obtained.Reproductivity of the FETEM characterization is excellent.Comparing with laser scattering method,which is liable to give positive error especially for small size particle anchoring disperser,FETEM characterization can give more accurate measurement of particle size.Moreover,FETEM can give dispersion feature of nanoparticle in non-aqueous medium.
