Traditional smoking of fish in Nigeria is often carried out in open and unsanitary environment. In addition, the current process does not result in uniformly smoked fish and uses wood (an increasingly scarce material...Traditional smoking of fish in Nigeria is often carried out in open and unsanitary environment. In addition, the current process does not result in uniformly smoked fish and uses wood (an increasingly scarce material) as heat source. The fish smoking kiln designed and fabricated in this study addresses these problems. The kiln (700 × 800 × 1,500 mm) is made of stainless steel on the inside and mild steel on the outside with glass fiber insulation separating the inside and outside covers. The sample to be smoked is totally enclosed by the kiln with a latchable door provided at one of the sides for placing of samples to be smoked. In addition, the kiln was designed to use waste biomass resource as fuels (sawdust and/or maizecob). Bonga fish (Ethmalosafimbriata) and African catfish (Clarias gariepinus) were used as test samples for the kiln. The temperature of the chamber increased to about 120 ℃ to 160 ℃ when fueled with sawdust and about 150 ℃ to 200 ℃ for maizecob. The total heat transfer resistance through the kiln walls was determined to be 1.0 ℃/W. About 6-8 h was required to smoke-dry 16 kg of fish samples from initial moisture content of 75% to about 25% or 20% moisture level.展开更多
为提高菇渣的利用效率,以菇渣为发酵原料,在35℃下,进行初始干物质浓度为6%、8%、10%和12%的厌氧发酵,考察了初始干物质浓度对产甲烷性能及有机质降解的影响。结果表明:初始干物质浓度在10%时发酵效果最佳,此时累计产甲烷量、VS产气率...为提高菇渣的利用效率,以菇渣为发酵原料,在35℃下,进行初始干物质浓度为6%、8%、10%和12%的厌氧发酵,考察了初始干物质浓度对产甲烷性能及有机质降解的影响。结果表明:初始干物质浓度在10%时发酵效果最佳,此时累计产甲烷量、VS产气率和VS产甲烷率分别为4 034.71 m L、235.15 m L·g^(-1)和134 m L·g^(-1),VS降解率为43.19%;菇渣的沼气产率与VS降解率成正比;淀粉、脂肪和蛋白质的降解率分别为99.01%、70.78%和17.16%;总纤维素的降解率为30.37%,以半纤维素降解为主。实验结果说明,菇渣具有较高的产气性能;产生的沼气主要来源于微生物对碳水化合物的降解。展开更多
Aims Plant litter decomposition is a key ecosystem process that determines carbon and nutrient cycling in terrestrial ecosystems.As a main component of litter,cellulose is a vital energy source for the microbes associ...Aims Plant litter decomposition is a key ecosystem process that determines carbon and nutrient cycling in terrestrial ecosystems.As a main component of litter,cellulose is a vital energy source for the microbes associated with litter decomposition.The important role of cellulolytic enzymes in litter cellulose degradation is well understood,but seasonal patterns of cellulose degradation and whether cumulative enzyme activities and litter quality forecast cellulose degradation in an alpine meadow remain elusive,which limits our understanding of cellulose degradation in herbaceous plant litter.Methods A two-year field litterbag experiment involving three dominant species(Ajuga ovalifolia,Festuca wallichanica,and Pedicularis roylei)was conducted in an alpine meadow of the eastern Tibetan Plateau to explore the seasonal patterns of cellulose degradation and how cumulative cellulolytic enzyme activities and initial litter quality impact cellulose degradation.Important findings Our study demonstrates that cellulose degraded rapidly and exceeded 50%during the first year,which mainly occurred in the first growing season(31.9%–43.3%).At two years of decomposition,cellulose degradation was driven by cumulative endoglucanase(R^(2)=0.70),cumulative cellobiohydrolase(R^(2)=0.59)and cumulative 1,4-β-glucosidase(R^(2)=0.57).In addition,the concentrations of cellulose,dissolved organic carbon,total phenol,lignin and lignin/N accounted for 52%–78%of the variation in cellulose degradation during the two years of decomposition.The best model for predicting cellulose degradation was the initial cellulose concentration(R^(2)=0.78).The enzymatic efficiencies and the allocation of cellulolytic enzyme activities were different among species.The cellulolytic enzyme efficiencies were higher in the litter of F.wallichanica with relatively lower quality.For the complete cellulose degradation of the leaf litter,A.ovalifolia and F.wallichanica required 4-fold and 6.7-fold more endoglucanase activity,3-fold and 4.5-fold more cellobiohydrolase activity and 1.2-fold and 1.4-fold more 1,4-β-glucosidase activity,respectively,than those required by P.roylei.Our results demonstrated that although microbial activity and litter quality both have significant impacts on cellulose degradation in an alpine meadow,using cellulose concentration to predict cellulose degradation is a good way to simplify the model of cellulose degradation and C cycling during litter decomposition.展开更多
Gas hydrate decomposition in sediments involves complicated multiphase flow and heat and mass transfer processes because of heat absorption by solid hydrates. Factors affecting gas hydrate decomposition in sediments i...Gas hydrate decomposition in sediments involves complicated multiphase flow and heat and mass transfer processes because of heat absorption by solid hydrates. Factors affecting gas hydrate decomposition in sediments include sediment type, mineral composition, pore size distribution, particle size, pore water composition, hydrate saturation distribution, initial formation pressure and temperature and cement characteristics. In this paper, experimental simulations of gas hydrate decomposition are carried out on an artificial core to investigate the effects of initial pressure and temperature, particle size and pore size. The experiments show that the characteristics of gas hydrate decomposition in sediments differ completely from those in a pure water system. The decomposition rate of hydrate sediments increases with the initial pressure increasing and decreasing temperatures. Furthermore, the decomposition rate of hydrate sediments decreases with decreasing particle size and increasing pore size.展开更多
文摘Traditional smoking of fish in Nigeria is often carried out in open and unsanitary environment. In addition, the current process does not result in uniformly smoked fish and uses wood (an increasingly scarce material) as heat source. The fish smoking kiln designed and fabricated in this study addresses these problems. The kiln (700 × 800 × 1,500 mm) is made of stainless steel on the inside and mild steel on the outside with glass fiber insulation separating the inside and outside covers. The sample to be smoked is totally enclosed by the kiln with a latchable door provided at one of the sides for placing of samples to be smoked. In addition, the kiln was designed to use waste biomass resource as fuels (sawdust and/or maizecob). Bonga fish (Ethmalosafimbriata) and African catfish (Clarias gariepinus) were used as test samples for the kiln. The temperature of the chamber increased to about 120 ℃ to 160 ℃ when fueled with sawdust and about 150 ℃ to 200 ℃ for maizecob. The total heat transfer resistance through the kiln walls was determined to be 1.0 ℃/W. About 6-8 h was required to smoke-dry 16 kg of fish samples from initial moisture content of 75% to about 25% or 20% moisture level.
文摘为提高菇渣的利用效率,以菇渣为发酵原料,在35℃下,进行初始干物质浓度为6%、8%、10%和12%的厌氧发酵,考察了初始干物质浓度对产甲烷性能及有机质降解的影响。结果表明:初始干物质浓度在10%时发酵效果最佳,此时累计产甲烷量、VS产气率和VS产甲烷率分别为4 034.71 m L、235.15 m L·g^(-1)和134 m L·g^(-1),VS降解率为43.19%;菇渣的沼气产率与VS降解率成正比;淀粉、脂肪和蛋白质的降解率分别为99.01%、70.78%和17.16%;总纤维素的降解率为30.37%,以半纤维素降解为主。实验结果说明,菇渣具有较高的产气性能;产生的沼气主要来源于微生物对碳水化合物的降解。
基金National Natural Science Foundation of China[31200345,31570605 and 31370628]China Scholarship Council(201706910039) to Y.C.(joint Ph.D.programme grant).
文摘Aims Plant litter decomposition is a key ecosystem process that determines carbon and nutrient cycling in terrestrial ecosystems.As a main component of litter,cellulose is a vital energy source for the microbes associated with litter decomposition.The important role of cellulolytic enzymes in litter cellulose degradation is well understood,but seasonal patterns of cellulose degradation and whether cumulative enzyme activities and litter quality forecast cellulose degradation in an alpine meadow remain elusive,which limits our understanding of cellulose degradation in herbaceous plant litter.Methods A two-year field litterbag experiment involving three dominant species(Ajuga ovalifolia,Festuca wallichanica,and Pedicularis roylei)was conducted in an alpine meadow of the eastern Tibetan Plateau to explore the seasonal patterns of cellulose degradation and how cumulative cellulolytic enzyme activities and initial litter quality impact cellulose degradation.Important findings Our study demonstrates that cellulose degraded rapidly and exceeded 50%during the first year,which mainly occurred in the first growing season(31.9%–43.3%).At two years of decomposition,cellulose degradation was driven by cumulative endoglucanase(R^(2)=0.70),cumulative cellobiohydrolase(R^(2)=0.59)and cumulative 1,4-β-glucosidase(R^(2)=0.57).In addition,the concentrations of cellulose,dissolved organic carbon,total phenol,lignin and lignin/N accounted for 52%–78%of the variation in cellulose degradation during the two years of decomposition.The best model for predicting cellulose degradation was the initial cellulose concentration(R^(2)=0.78).The enzymatic efficiencies and the allocation of cellulolytic enzyme activities were different among species.The cellulolytic enzyme efficiencies were higher in the litter of F.wallichanica with relatively lower quality.For the complete cellulose degradation of the leaf litter,A.ovalifolia and F.wallichanica required 4-fold and 6.7-fold more endoglucanase activity,3-fold and 4.5-fold more cellobiohydrolase activity and 1.2-fold and 1.4-fold more 1,4-β-glucosidase activity,respectively,than those required by P.roylei.Our results demonstrated that although microbial activity and litter quality both have significant impacts on cellulose degradation in an alpine meadow,using cellulose concentration to predict cellulose degradation is a good way to simplify the model of cellulose degradation and C cycling during litter decomposition.
基金supported by the National Basic Research Program of China (Grant No. 2009CB219507)
文摘Gas hydrate decomposition in sediments involves complicated multiphase flow and heat and mass transfer processes because of heat absorption by solid hydrates. Factors affecting gas hydrate decomposition in sediments include sediment type, mineral composition, pore size distribution, particle size, pore water composition, hydrate saturation distribution, initial formation pressure and temperature and cement characteristics. In this paper, experimental simulations of gas hydrate decomposition are carried out on an artificial core to investigate the effects of initial pressure and temperature, particle size and pore size. The experiments show that the characteristics of gas hydrate decomposition in sediments differ completely from those in a pure water system. The decomposition rate of hydrate sediments increases with the initial pressure increasing and decreasing temperatures. Furthermore, the decomposition rate of hydrate sediments decreases with decreasing particle size and increasing pore size.