Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary cerebrovascular disease leading to accumulating neurologic deficits and dementia. CADASIL has been l...Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary cerebrovascular disease leading to accumulating neurologic deficits and dementia. CADASIL has been linked to nucleotide substitutions and deletions in the Notch3 gene. All the mutations described until now lead to unpaired cysteine residue in the epidermal growth factor like repeats. The authors report a family with CADASIL carrying a deletion in the Notch3 gene that did not involve a cysteine residue.展开更多
Peroxiredoxins(Prxs) are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species(ROS). Although extensive efforts have been made over the la...Peroxiredoxins(Prxs) are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species(ROS). Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye(Leymus Chinensis), designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H2O2 and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na2CO3 in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na2CO3.展开更多
γ-Gliadins are an important component of wheat seed storage proteins.Four novelγ-gliadin genes(Gli-ng1 to Gli-ng4)were cloned from wheat(Triticum aestivum)and Aegilops species.The novelγ-gliadins were much smaller ...γ-Gliadins are an important component of wheat seed storage proteins.Four novelγ-gliadin genes(Gli-ng1 to Gli-ng4)were cloned from wheat(Triticum aestivum)and Aegilops species.The novelγ-gliadins were much smaller in molecular size when compared to the typicalγ-gliadins,which was caused by deletion of the non-repetitive domain,glutamine-rich region,3′part of the repetitive domain,and 5′part of the C-terminal,possibly due to illegitimate recombination between the repetitive domain and the C-terminal.As a result,Gli-ng1 and Gli-ng4 only contained two and three cysteine residues,respectively.Gli-ng1,as the representative of novelγ-gliadin genes,has been sub-cloned into an Escherichia coli expression system.SDSPAGE indicated that the both cysteine residues of Gli-ng1 could participate in the formation of intermolecular disulphide bonds in vitro.Successful cloning of Gli-ng1 from seed cDNA of T.aestivum cv.Chinese Spring suggested that these novelγ-gliadin genes were normally transcribed during the development of seeds.Phylogenic analysis indicated that the four novelγ-gliadin genes had a closer relationship with those from the B(S)genome of wheat.展开更多
文摘Cerebral autosomal dominant arteriopathy with subcortical infarcts and leukoencephalopathy (CADASIL) is a hereditary cerebrovascular disease leading to accumulating neurologic deficits and dementia. CADASIL has been linked to nucleotide substitutions and deletions in the Notch3 gene. All the mutations described until now lead to unpaired cysteine residue in the epidermal growth factor like repeats. The authors report a family with CADASIL carrying a deletion in the Notch3 gene that did not involve a cysteine residue.
基金Acknowledgments We are grateful to Dr DA Melton （Harvard University） for shar- ing his human ES cells with us. The study was supported by grants from the National High Technology Research and Development Program of China （2006CB943900）, the National Natural Science Foundation of China （General Program, 30500088）, the Shang- hai Jiao Tong University School of Medicine, and the Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences. The study was also
supported by the Shanghai Leading Academic Deciline Project （S30201）.
基金Supported by the Transgenic Plant Research Special Program of China(No.2008ZX08003-005)the Technology Development Project of Jilin Province,China(Nos.20086029,20076016)
文摘Peroxiredoxins(Prxs) are a large family of antioxidant enzymes of various types that take part in signal transduction via decomposing reactive oxygen species(ROS). Although extensive efforts have been made over the last decades in understanding the structures and functions of Prxs, type II Prxs in monocots are hardly studied. In this work, a monocot type II Prx gene homologue from Chinese wildrye(Leymus Chinensis), designated as LcTpxII, was isolated and characterized. LcTpxII encoding a 162-amino acid protein contains a thioredoxin domain and a cysteine residue at position 51, suggesting that it is a member of the Prxs family. The LcTpxII is capable of decomposing H2O2 and protecting plasmid DNA from damage caused by ROS. The expression of LcTpxII in Chinese wildrye was induced by 400 mmol/L NaCl and 100 mmol/L Na2CO3 in the experiment. The overexpression of LcTpxII enhances the tolerance of transgenic yeast to 1.6 mol/L NaCl and 10 mmol/L Na2CO3.
基金finically supported by the National Natural Science Foundation of China (31230053)
文摘γ-Gliadins are an important component of wheat seed storage proteins.Four novelγ-gliadin genes(Gli-ng1 to Gli-ng4)were cloned from wheat(Triticum aestivum)and Aegilops species.The novelγ-gliadins were much smaller in molecular size when compared to the typicalγ-gliadins,which was caused by deletion of the non-repetitive domain,glutamine-rich region,3′part of the repetitive domain,and 5′part of the C-terminal,possibly due to illegitimate recombination between the repetitive domain and the C-terminal.As a result,Gli-ng1 and Gli-ng4 only contained two and three cysteine residues,respectively.Gli-ng1,as the representative of novelγ-gliadin genes,has been sub-cloned into an Escherichia coli expression system.SDSPAGE indicated that the both cysteine residues of Gli-ng1 could participate in the formation of intermolecular disulphide bonds in vitro.Successful cloning of Gli-ng1 from seed cDNA of T.aestivum cv.Chinese Spring suggested that these novelγ-gliadin genes were normally transcribed during the development of seeds.Phylogenic analysis indicated that the four novelγ-gliadin genes had a closer relationship with those from the B(S)genome of wheat.
基金This work was supported by the National Natural Science Foundation of China （No.21202006）, the Fundamental Research Funds for the Central Universities of Ministry of Education of China （No.FRF-TP- 14-015A2）, the Natural Science Foundation of Anhui Province （No.1308085QB38）, and the Supercomputer Centre of Shanghai.
