[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF wa...[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.展开更多
An enantioselective umpolung γ-addition reaction of 3’-indolyl-3-oxindoles to allenoates catalyzed by amino acid-derived bifunctional phosphine catalysts has been developed. A wide range of chiral mixed 3,3’-bisind...An enantioselective umpolung γ-addition reaction of 3’-indolyl-3-oxindoles to allenoates catalyzed by amino acid-derived bifunctional phosphine catalysts has been developed. A wide range of chiral mixed 3,3’-bisindole scaffolds containing an all-carbon quaternary stereogenic center were obtained in high yields and with excellent enantioselectivities. 3,3’-Bisindoles are valuable synthetic intermediates, the employment of which led to formal total syntheses of(+)-Chimonanthine,(+)-Folicanthine and(-)-Calycanthine, as well as facile creation of useful pyrrolidinoindoline core structure.展开更多
A variety of unique Al(salen) complexes functionalized by imidazolium-based ionic liquid(IL) moieties with the salen ligand at the two sides of 3,3′-position have been successfully prepared, rather than familiar 5,5...A variety of unique Al(salen) complexes functionalized by imidazolium-based ionic liquid(IL) moieties with the salen ligand at the two sides of 3,3′-position have been successfully prepared, rather than familiar 5,5′-position reported previously.The catalytic activity obtained by these bifunctional catalysts could be superior to those of the binary type catalysts in the formation of five-membered heterocyclic compounds from the cycloaddition reaction of CO_2 and three-membered heterocyclic compounds(including terminal epoxides and N-substituted aziridines), presumably due to the distinguished intramolecularly synergistic catalysis, which might lead to perform the cycloaddition reaction at ambient conditions and retain excellent yield and unprecedented chemo-or regioselectivity. Moreover, the polyether-based trifunctional Al(salen) catalysts with the best catalytic performance could be regenerated and reused at least eight times without any obvious decreases in catalytic activity. Finally,the kinetic investigation suggested the structure of catalysts had important influences on the catalytic activity, thereby proposing the possible reaction mechanism.展开更多
基金Supported by Bioreactor Important Special Item of 863-Program inthe "Eleventh Five-Year" Plan (No. 2007AA100503)Science and Technology Development Key Plan of Jilin Province( No.20070922)+1 种基金Cultivation Fund of Scientific and Technical Innovation Project Major Program of Higher Education Institutions ( No.70S018)Science and Technology Plan of Changchun City (No.06GG150)~~
文摘[ Objective] The study is to generate pharmaceutical protein via plant transgenic technique. [Methed] Using the cotyledons with petiole as transformation receptor, the fusion gene of rapeseed oil-body gene and bFGF was introduced into the rapeseed （ Brassica campestris L. ） by Agrobacterium tumefaciens-mediated transformation; meanwhile regeneration conditions of rapeseed were also optimized, and the regenerated resistant plantlets were detected by PCR and Southern blot. [ Result] This fusion gene had been integrated into rapeseed genome successfully, and the optimized conditions of transformation and regeneration were as follows： explants pre-culture for 2 d, co-culture for 3 d, bacteria solution OD600 for 0.3 and infection time for 5 min. [ Conclusion] The results laid a solid foundation for extraction, isolation and purification of protein in transgenic plant seeds.
基金Yixin Lu thanks the Singapore National Research Foundation,Prime Minister’s Office for the NRF Investigatorship Award(R-143-000-A15-281)Financial supports from the National University of Singapore(R-143-000-695-114 and C-141-000-092-001)the National Natural Science Foundation of China(21672158)are also gratefully acknowledged.
文摘An enantioselective umpolung γ-addition reaction of 3’-indolyl-3-oxindoles to allenoates catalyzed by amino acid-derived bifunctional phosphine catalysts has been developed. A wide range of chiral mixed 3,3’-bisindole scaffolds containing an all-carbon quaternary stereogenic center were obtained in high yields and with excellent enantioselectivities. 3,3’-Bisindoles are valuable synthetic intermediates, the employment of which led to formal total syntheses of(+)-Chimonanthine,(+)-Folicanthine and(-)-Calycanthine, as well as facile creation of useful pyrrolidinoindoline core structure.
基金supported by the National Science for Distinguished Young Scholars of China(21425627)the National Natural Science Foundation of China(21676306)+1 种基金the Natural Science Foundation of Guangdong Province(2016A030310211,2015A030313104)the Fundamental Research Funds for the Central Universities of Sun Yat-sen University
文摘A variety of unique Al(salen) complexes functionalized by imidazolium-based ionic liquid(IL) moieties with the salen ligand at the two sides of 3,3′-position have been successfully prepared, rather than familiar 5,5′-position reported previously.The catalytic activity obtained by these bifunctional catalysts could be superior to those of the binary type catalysts in the formation of five-membered heterocyclic compounds from the cycloaddition reaction of CO_2 and three-membered heterocyclic compounds(including terminal epoxides and N-substituted aziridines), presumably due to the distinguished intramolecularly synergistic catalysis, which might lead to perform the cycloaddition reaction at ambient conditions and retain excellent yield and unprecedented chemo-or regioselectivity. Moreover, the polyether-based trifunctional Al(salen) catalysts with the best catalytic performance could be regenerated and reused at least eight times without any obvious decreases in catalytic activity. Finally,the kinetic investigation suggested the structure of catalysts had important influences on the catalytic activity, thereby proposing the possible reaction mechanism.
