目的:基于网络药理学探讨双心方治疗心肌梗死后抑郁的作用机制。方法:通过中药系统药理学数据库及分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)获取双心方活性成分及其对应靶点...目的:基于网络药理学探讨双心方治疗心肌梗死后抑郁的作用机制。方法:通过中药系统药理学数据库及分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)获取双心方活性成分及其对应靶点,GeneCards、PharmGKB数据库搜集心肌梗死与抑郁的相关靶点并整合,将药物靶点与疾病靶点取交集后得到双心方的潜在作用靶点。使用Cytoscape 3.8.2软件构建药物—成分—潜在作用靶点网络,利用STRING数据库进行蛋白质相互作用(protein-protein interaction, PPI)网络分析,并利用DAVID V6.8数据库进行基因本体论(gene ontology, GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析。结果:获得双心方的有效成分88个,潜在作用靶点89个,核心靶点10个。通过GO和KEGG富集分析分别获得229条生物学过程条目和34条信号通路。通过HIF-1、PI3K/Akt等多条信号通路介导,双心方中的多重活性成分有心脏保护作用,可通过抗心肌细胞凋亡、抗炎、抗氧化应激反应,促进心肌梗死后心脏内的血管生成等治疗心肌梗死及改善预后,通过抑制神经元的氧化应激损伤、减少炎症介导的细胞毒性和神经元死亡等改善抑郁。结论:双心方主要是通过多靶点、多通路来发挥治疗心肌梗死后抑郁的作用,该研究为后续深入开展双心方治疗心肌梗死后抑郁的作用机制研究提供了依据与方向。展开更多
Pepino mosaic virus(PepMV)causes severe disease in tomato and other Solanaceous crops around globe.To effectively study and manage this viral disease,researchers need new,sensitive,and high-throughput approaches for v...Pepino mosaic virus(PepMV)causes severe disease in tomato and other Solanaceous crops around globe.To effectively study and manage this viral disease,researchers need new,sensitive,and high-throughput approaches for viral detection.In this study,we purified PepMV particles from the infected Nicotiana benthamiana plants and used virions to immunize BALB/c mice to prepare hybridomas secreting anti-PepMV monoclonal antibodies(mAbs).A panel of highly specific and sensitive murine mAbs(15B2,8H6,23D11,20D9,3A6,and 8E3)could be produced through cell fusion,antibody selection,and cell cloning.Using the mAbs as the detection antibodies,we established double antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA),Dot-ELISA,and Tissue print-ELISA for detecting PepMV infection in tomato plants.Resulting data on sensitivity analysis assays showed that both DAS-ELISA and Dot-ELISA can efficiently monitor the virus in PepMV-infected tissue crude extracts when diluted at 1:1310720 and 1:20480(weight/volume ratio(w/v),g/mL),respectively.Among the three methods developed,the Tissue print-ELISA was found to be the most practical detection technique.Survey results from field samples by the established serological approaches were verified by reverse transcription polymerase chain reaction(RT-PCR)and DNA sequencing,dem on strati ng all three serological methods are reliable and effective for monitoring PepMV.An ti-PepMV mAbs and the newly developed DAS-ELISA,Dot-ELISA,and Tissue print-ELISA can benefit PepMV detection and field epidemiological study,and management of this viral disease,which is already widespread in tomato plants in Yunnan Province of China.展开更多
We solve a generalized nonautonomous nonlinear Schrodinger equation analytically by performing the Hirota＇s bilinearization method. The precise expression of a parameter e, which provides a compatibility condition an...We solve a generalized nonautonomous nonlinear Schrodinger equation analytically by performing the Hirota＇s bilinearization method. The precise expression of a parameter e, which provides a compatibility condition and dark soliton management, is obtained. Comparing with nonautonomous bright soliton, we find that the gain parameter affects both the background and the valley of dark soliton （∈2 ≠ 1） while it has no effects on the wave central position. Moreover, the precise expressions of a nonautonomous black soliton＇s （∈2 = 1） width, background and the trajectory of its wave central, which describe the dynamic behavior of soliton＇s evolution, are investigated analytically. Finally, the stability of the dark soliton solution is demonstrated numerically. It is shown that the main characteristic of the dark solitons keeps unchanged under a slight perturbation in the compatibility condition.展开更多
文摘目的:基于网络药理学探讨双心方治疗心肌梗死后抑郁的作用机制。方法:通过中药系统药理学数据库及分析平台(traditional Chinese medicine systems pharmacology database and analysis platform, TCMSP)获取双心方活性成分及其对应靶点,GeneCards、PharmGKB数据库搜集心肌梗死与抑郁的相关靶点并整合,将药物靶点与疾病靶点取交集后得到双心方的潜在作用靶点。使用Cytoscape 3.8.2软件构建药物—成分—潜在作用靶点网络,利用STRING数据库进行蛋白质相互作用(protein-protein interaction, PPI)网络分析,并利用DAVID V6.8数据库进行基因本体论(gene ontology, GO)和京都基因与基因组百科全书(kyoto encyclopedia of genes and genomes, KEGG)富集分析。结果:获得双心方的有效成分88个,潜在作用靶点89个,核心靶点10个。通过GO和KEGG富集分析分别获得229条生物学过程条目和34条信号通路。通过HIF-1、PI3K/Akt等多条信号通路介导,双心方中的多重活性成分有心脏保护作用,可通过抗心肌细胞凋亡、抗炎、抗氧化应激反应,促进心肌梗死后心脏内的血管生成等治疗心肌梗死及改善预后,通过抑制神经元的氧化应激损伤、减少炎症介导的细胞毒性和神经元死亡等改善抑郁。结论:双心方主要是通过多靶点、多通路来发挥治疗心肌梗死后抑郁的作用,该研究为后续深入开展双心方治疗心肌梗死后抑郁的作用机制研究提供了依据与方向。
基金National Key R&D Program of China(Nos.2019YFD1001800 and 2017YFD0201604)the National Natural Science Foundation of China(Nos.31772125 and 31972234)。
文摘Pepino mosaic virus(PepMV)causes severe disease in tomato and other Solanaceous crops around globe.To effectively study and manage this viral disease,researchers need new,sensitive,and high-throughput approaches for viral detection.In this study,we purified PepMV particles from the infected Nicotiana benthamiana plants and used virions to immunize BALB/c mice to prepare hybridomas secreting anti-PepMV monoclonal antibodies(mAbs).A panel of highly specific and sensitive murine mAbs(15B2,8H6,23D11,20D9,3A6,and 8E3)could be produced through cell fusion,antibody selection,and cell cloning.Using the mAbs as the detection antibodies,we established double antibody sandwich enzyme-linked immunosorbent assay(DAS-ELISA),Dot-ELISA,and Tissue print-ELISA for detecting PepMV infection in tomato plants.Resulting data on sensitivity analysis assays showed that both DAS-ELISA and Dot-ELISA can efficiently monitor the virus in PepMV-infected tissue crude extracts when diluted at 1:1310720 and 1:20480(weight/volume ratio(w/v),g/mL),respectively.Among the three methods developed,the Tissue print-ELISA was found to be the most practical detection technique.Survey results from field samples by the established serological approaches were verified by reverse transcription polymerase chain reaction(RT-PCR)and DNA sequencing,dem on strati ng all three serological methods are reliable and effective for monitoring PepMV.An ti-PepMV mAbs and the newly developed DAS-ELISA,Dot-ELISA,and Tissue print-ELISA can benefit PepMV detection and field epidemiological study,and management of this viral disease,which is already widespread in tomato plants in Yunnan Province of China.
基金Supported by the National Natural Science Foundation of China under Grant Nos. 10975180, 11047025, and 11075126 and the Applied nonlinear Science and Technology from the Most Important Among all the Top Priority Disciplines of Zhejiang Province
文摘We solve a generalized nonautonomous nonlinear Schrodinger equation analytically by performing the Hirota＇s bilinearization method. The precise expression of a parameter e, which provides a compatibility condition and dark soliton management, is obtained. Comparing with nonautonomous bright soliton, we find that the gain parameter affects both the background and the valley of dark soliton （∈2 ≠ 1） while it has no effects on the wave central position. Moreover, the precise expressions of a nonautonomous black soliton＇s （∈2 = 1） width, background and the trajectory of its wave central, which describe the dynamic behavior of soliton＇s evolution, are investigated analytically. Finally, the stability of the dark soliton solution is demonstrated numerically. It is shown that the main characteristic of the dark solitons keeps unchanged under a slight perturbation in the compatibility condition.
