By analyzing the field population of Brandt’s vole,it reveals that the daily non-feeding grass consumption is about 2.81g (in dry) or 7.22g (in fresh) per vole.There is about 11 percent of the gnawed grass will be re...By analyzing the field population of Brandt’s vole,it reveals that the daily non-feeding grass consumption is about 2.81g (in dry) or 7.22g (in fresh) per vole.There is about 11 percent of the gnawed grass will be re-utilized by the vole within 24 hours.The non-feeding grass consumption of this species is much more lower than its daily food consumption.展开更多
Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to f...Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to find the genuine reason leading to effective resistance response of tree to insects attacking. The release situation of the induced volatiles of the plant was analyzed by TCT-GC/MS at 24 hours after damages. The results indicated that some of the volatiles such as (Z)-3-hexenyl acetate, decanal, 3-hexenyl isovalerate, nonanal, ocimene, and 2-cyanobutane can be induced by both insects attack-ing and mechanical damage, while 2,6-dimethyl-1,3,5,7-octatetraene, 2-methyl-6-methylene-1,7-octadien-3-one, caryophyllene, Isovaleronitrile, diethyl-methyl-benzamide, and dicapryl phthalate were only induced by insects attacking. Such difference in volatiles was attributed to that there existed active components in oral sections of the larvae of Lymantria dispar展开更多
AIM： To observe the effects of traditional antiinflammatory medicine Lonicerae FIos （LF） on rat reflux esophagitis （RE） induced by pylorus and forestomach ligation compared with the well-known proton antioxidant,...AIM： To observe the effects of traditional antiinflammatory medicine Lonicerae FIos （LF） on rat reflux esophagitis （RE） induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, α-tocopherol. METHODS： Rats were pretreated with three different dosages of LF （500, 250 and 125 mg/kg） orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA）, myeloperoxidase and glutathione （GSH） levels, and collagen contents （marker of flexibility） were observed on the esophageal and fundic histopathology. The results were compared with an α-tocopherol （once orally, 1 h before operation, 30 mg/kg） treated group in which the effects on RE were already confirmed.RESULTS： Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects α-tocopherol. CONCLUSION： The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.展开更多
Response surface methodology was used in this research to optimize the technical parameters of the extraction and conversion color compound in armatto seeds into norbixin. Parameters affected the extraction efficiency...Response surface methodology was used in this research to optimize the technical parameters of the extraction and conversion color compound in armatto seeds into norbixin. Parameters affected the extraction efficiency and norbixin recovery yield were solvent concentration, rate of solvent/seed, incubation time, and incubation temperature. The optimization results including： concentration of ethanol is 51.82°; concentration of NaOH is 0.52 M; ratio of solvent/seed is 7.1/1; incubation time is 33.12 minutes, incubation temperature is 58.6℃, and the rate of 36% HCl/color solution is 0.5/5 （v/v）.展开更多
[ Objective] The aim was to study the optimum extraction condition of dietary fiber of wheat bran and to discuss its influence on viscosity-elasticity of noodle dough with added extracts. [ Methed] Influences of a-amy...[ Objective] The aim was to study the optimum extraction condition of dietary fiber of wheat bran and to discuss its influence on viscosity-elasticity of noodle dough with added extracts. [ Methed] Influences of a-amylase, alkaline concentration, alkaline hydrolysis time and temperature on water holding capability and swelling capacity of dietary fiber were evaluated using single-factor test and orthogonal test. Effects of added dietary fiber from wheat bran on dough absorption ratio, rupture stress of dough and creep resistance were studied. [ Result] When the hydrolysis condi-tions of wheat bran were 0.4% a-amylase at 75 ℃ for 60 rain, further alkaline conditions were 5% sodium hydroxide for 60 min at 65 ℃, dietary fi-ber exhibited fine water holding capability and swelling capacity. The addition of 3%-5% dietary fiber from wheat bran into dough had little influence on the water absorption ratio of noodle, rupture stress of dough, creep resistance and can make functional noodle with rich dietary fiber from wheat bran. [Coonclusion] The study provided reference for the comprehensive utilization of wheat bran and development of functional product.展开更多
Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and...Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and purification of bioactive principle from a complex matrix is often inherent bottleneck for the utilization of natural products,so a series of extraction and separation techniques have been developed.This review covers recent advances in the separation of bioactive natural products with an emphasis on their solubility and diffusion coefficients,recent extraction techniques and isolation techniques.This overview of recent technological advances,discussion of pertinent problems and prospect of current methodologies in the separation of bioactive natural products may provide a driving force for development of novel separation techniques.展开更多
[ Objective] To optimize the extraction conditions of insoluble dietary fiber from apple pulp. [ Method] The insoluble dietary fiber was ex-tracted from the apple pulp by alkaline, and the optimal extraction condition...[ Objective] To optimize the extraction conditions of insoluble dietary fiber from apple pulp. [ Method] The insoluble dietary fiber was ex-tracted from the apple pulp by alkaline, and the optimal extraction conditions were determined by orthogonal experiment. [ Result] The influential or-der of each factor on the extraction of insoluble fiber decreased in the order of solid-liquid ratio 〉 alkali concentration 〉 temperature 〉 extraction time, and the optimal extraction technology was A3 B1 C2 D2, that is, 1：11 （g/ml） of solid-liquid ratio, 0.25 mol/L alkaline concentration, 50 ℃ of extraction temperature and 2.0 h extraction time, under these conditions, the yield of insoluble dietary fiber was 35.46%. [ Conchsion] The apple pulp contains rich dietary fiber, and the insoluble dietary fiber extracted from the apple pulp has great development value. Key words Apple; Insoluble dietary fiber; Extraction technology; China展开更多
Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of defenses.We have investigated whether plants that have few mechanical ...Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of defenses.We have investigated whether plants that have few mechanical defenses,but have extrafloral nectaries(EFNs)patrolled by ants,are less prone to herbivory,compared with plants without EFNs that have tougher leaves.Methods Data from the literature were extracted to examine the reported levels of herbivory in plants with or without EFNs.In a savanna vegetation in southern Brazil,field data were collected in leaves from six tropical species and herbivory and specific leaf area(SLA)levels were measured.We further evaluated differences in herbivory and SLA among species and between plants with or without EFNs.In order to test the relationship between herbivory and leaf toughness we regressed average herbivory and average SLA per plant.Important Findings Plants exhibited variable levels of leaf damage,but plants without ant defenses experienced the highest levels of leaf area loss to herbivory.Levels of mechanical defenses were also variable among the plant species.Plants without EFNs were tougher,exhibiting lower values of SLA.Although plants without EFNs had more sclerophyllous leaves,this mechanical defense was not sufficient to impair and/or reduce herbivore feeding,suggesting that the biotic defenses performed by patrolling ants might be more effective than investment in mechanical defenses associated with leaf palatability.展开更多
In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglecte...In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be at- tributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ13c and δ15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range （2.9-3.6）, the mean difference of δ13C value was only （0.3±0.2）%o （n=2） while δ15N value was （0.6±0.1）‰ （n=2）. Although the atomic C/N ratios of some SC are slightly be- yond the normal range, the mean differences of δ13C and δ15N values were still only （0.4±0.1）‰and （0.3±0）‰ （n=2） respec- tively. These isotopic differences are quite below the isotope fractionation in one trophic level δ13C values of 1‰-1.5‰ and 615N values of 3‰-5‰）, suggesting that SC had great application potentials in palaeodietary research.展开更多
文摘By analyzing the field population of Brandt’s vole,it reveals that the daily non-feeding grass consumption is about 2.81g (in dry) or 7.22g (in fresh) per vole.There is about 11 percent of the gnawed grass will be re-utilized by the vole within 24 hours.The non-feeding grass consumption of this species is much more lower than its daily food consumption.
基金This research is supported by National Natural Science Foundation of China (No.30170764) and the Postgraduate Training Fund of Graduate School of Beijing Forestry University (No.03SW004)
文摘Three treatments including mechanical damage, Lymantria dispar attacking and daubing oral secretions of the in-sects on mechanically damaged cut were conducted on Populus simonii譖opulus pyramibalis c.v. in order to find the genuine reason leading to effective resistance response of tree to insects attacking. The release situation of the induced volatiles of the plant was analyzed by TCT-GC/MS at 24 hours after damages. The results indicated that some of the volatiles such as (Z)-3-hexenyl acetate, decanal, 3-hexenyl isovalerate, nonanal, ocimene, and 2-cyanobutane can be induced by both insects attack-ing and mechanical damage, while 2,6-dimethyl-1,3,5,7-octatetraene, 2-methyl-6-methylene-1,7-octadien-3-one, caryophyllene, Isovaleronitrile, diethyl-methyl-benzamide, and dicapryl phthalate were only induced by insects attacking. Such difference in volatiles was attributed to that there existed active components in oral sections of the larvae of Lymantria dispar
文摘AIM： To observe the effects of traditional antiinflammatory medicine Lonicerae FIos （LF） on rat reflux esophagitis （RE） induced by pylorus and forestomach ligation compared with the well-known proton antioxidant, α-tocopherol. METHODS： Rats were pretreated with three different dosages of LF （500, 250 and 125 mg/kg） orally, once a day for 14 d before pylorus and forestomach ligation. Nine hours after pylorus and forestomach ligation, changes to the stomach and esophagus lesion areas, gastric volumes, acid and pepsin outputs, antioxidant effects, esophageal lipid peroxidation, superoxide dismutase （SOD）, catalase （CAT）, malondialdehyde （MDA）, myeloperoxidase and glutathione （GSH） levels, and collagen contents （marker of flexibility） were observed on the esophageal and fundic histopathology. The results were compared with an α-tocopherol （once orally, 1 h before operation, 30 mg/kg） treated group in which the effects on RE were already confirmed.RESULTS： Pylorus and forestomach ligations caused marked increases of gross esophageal and gastric mucosa lesion areas, which corresponded with histopathological changes. In addition, increases of esophageal lipid peroxidation, decreases of SOD, CAT, and GSH-free radical scavengers, increases of collagen were observed. However, these pylorus and forestomach ligation induced RE were dose-dependently inhibited by treatment of 500, 250 and 125 mg/kg of LF extract, mediated by antioxidant effects. RE at 250 mg/kg showed similar effects α-tocopherol. CONCLUSION： The results suggest that antioxidant effects of LF could attenuate the severity of RE and prevent the esophageal mucosal damage, and validate its therapeutic use in esophageal reflux disease.
文摘Response surface methodology was used in this research to optimize the technical parameters of the extraction and conversion color compound in armatto seeds into norbixin. Parameters affected the extraction efficiency and norbixin recovery yield were solvent concentration, rate of solvent/seed, incubation time, and incubation temperature. The optimization results including： concentration of ethanol is 51.82°; concentration of NaOH is 0.52 M; ratio of solvent/seed is 7.1/1; incubation time is 33.12 minutes, incubation temperature is 58.6℃, and the rate of 36% HCl/color solution is 0.5/5 （v/v）.
基金Supported by National Science and Technology Fund(31171753)International Science and Technology Cooperation Program of Anhui Province(10080703035)Natural Scientific Research Fund of Universities in Anhui Province(KJ2009A109)
文摘[ Objective] The aim was to study the optimum extraction condition of dietary fiber of wheat bran and to discuss its influence on viscosity-elasticity of noodle dough with added extracts. [ Methed] Influences of a-amylase, alkaline concentration, alkaline hydrolysis time and temperature on water holding capability and swelling capacity of dietary fiber were evaluated using single-factor test and orthogonal test. Effects of added dietary fiber from wheat bran on dough absorption ratio, rupture stress of dough and creep resistance were studied. [ Result] When the hydrolysis condi-tions of wheat bran were 0.4% a-amylase at 75 ℃ for 60 rain, further alkaline conditions were 5% sodium hydroxide for 60 min at 65 ℃, dietary fi-ber exhibited fine water holding capability and swelling capacity. The addition of 3%-5% dietary fiber from wheat bran into dough had little influence on the water absorption ratio of noodle, rupture stress of dough, creep resistance and can make functional noodle with rich dietary fiber from wheat bran. [Coonclusion] The study provided reference for the comprehensive utilization of wheat bran and development of functional product.
基金Supported by the National＇Natural Science Foundation of China （20936005, 21076175 and 21076178）, the National High Technology Research and Development Program of China （2012AA040211）, and the Program for Zhejiang Leading Team of S＆T Innovation （2011R50002）.
文摘Bioactive natural products are a main source of new drugs,functional foods and food additives.The separation of bioactive natural products plays an important role in transformation and use of biomass.The isolation and purification of bioactive principle from a complex matrix is often inherent bottleneck for the utilization of natural products,so a series of extraction and separation techniques have been developed.This review covers recent advances in the separation of bioactive natural products with an emphasis on their solubility and diffusion coefficients,recent extraction techniques and isolation techniques.This overview of recent technological advances,discussion of pertinent problems and prospect of current methodologies in the separation of bioactive natural products may provide a driving force for development of novel separation techniques.
文摘[ Objective] To optimize the extraction conditions of insoluble dietary fiber from apple pulp. [ Method] The insoluble dietary fiber was ex-tracted from the apple pulp by alkaline, and the optimal extraction conditions were determined by orthogonal experiment. [ Result] The influential or-der of each factor on the extraction of insoluble fiber decreased in the order of solid-liquid ratio 〉 alkali concentration 〉 temperature 〉 extraction time, and the optimal extraction technology was A3 B1 C2 D2, that is, 1：11 （g/ml） of solid-liquid ratio, 0.25 mol/L alkaline concentration, 50 ℃ of extraction temperature and 2.0 h extraction time, under these conditions, the yield of insoluble dietary fiber was 35.46%. [ Conchsion] The apple pulp contains rich dietary fiber, and the insoluble dietary fiber extracted from the apple pulp has great development value. Key words Apple; Insoluble dietary fiber; Extraction technology; China
基金supported by Coordenação de Aperfeiçoamento de Pessoal de Nível Superior-Brasil(CAPES)-(Finance Code 001)Conselho Nacional de Desenvolvimento Científico e Tecnológico(307210/2016-2).
文摘Aims Plants have limited resources for defenses and species that invest in biotic defenses might exhibit leaves that invest less in other types of defenses.We have investigated whether plants that have few mechanical defenses,but have extrafloral nectaries(EFNs)patrolled by ants,are less prone to herbivory,compared with plants without EFNs that have tougher leaves.Methods Data from the literature were extracted to examine the reported levels of herbivory in plants with or without EFNs.In a savanna vegetation in southern Brazil,field data were collected in leaves from six tropical species and herbivory and specific leaf area(SLA)levels were measured.We further evaluated differences in herbivory and SLA among species and between plants with or without EFNs.In order to test the relationship between herbivory and leaf toughness we regressed average herbivory and average SLA per plant.Important Findings Plants exhibited variable levels of leaf damage,but plants without ant defenses experienced the highest levels of leaf area loss to herbivory.Levels of mechanical defenses were also variable among the plant species.Plants without EFNs were tougher,exhibiting lower values of SLA.Although plants without EFNs had more sclerophyllous leaves,this mechanical defense was not sufficient to impair and/or reduce herbivore feeding,suggesting that the biotic defenses performed by patrolling ants might be more effective than investment in mechanical defenses associated with leaf palatability.
基金supported by CAS Strategic Priority Research Program (Grant No. XDA05130303)Chinese Academy of Sciences & Max-Planck Institute Partnership Group Project (Grant No. KACX1-YW-0830)
文摘In current palaeodietary research, gelatinization is the main method to extract insoluble collagen （ISC） from ancient bones. However, the degradation products of ISC, i.e., soluble collagen （SC）, is often neglected and abandoned. In this work, we try to separate the extracts of ancient bones using gel chromatography and compare the contents of carbon and nitrogen, atomic C/N ratio, and stable carbon and nitrogen isotopic values of the extracts from three peaks to determine which peak can be at- tributed to SC. At last, the potential application of SC in palaeodietary research is discussed based on the comparison of stable isotopic values between ISC and SC. Among the three peaks, the second with the retention time between 17.5 min and 27.5 min had the most broad peak shape, indicating that the molecular weights of proteins collected were most variable. Besides, the contents of carbon and nitrogen and atomic C/N ratio of extracts in this peak were closest to the corresponding ISC. Based on the above, we conclude that the extract in second peak is SC. More important, the δ13c and δ15N values of ISC and SC are very similar. For ISC and SC with atomic C/N ratios within the normal range （2.9-3.6）, the mean difference of δ13C value was only （0.3±0.2）%o （n=2） while δ15N value was （0.6±0.1）‰ （n=2）. Although the atomic C/N ratios of some SC are slightly be- yond the normal range, the mean differences of δ13C and δ15N values were still only （0.4±0.1）‰and （0.3±0）‰ （n=2） respec- tively. These isotopic differences are quite below the isotope fractionation in one trophic level δ13C values of 1‰-1.5‰ and 615N values of 3‰-5‰）, suggesting that SC had great application potentials in palaeodietary research.
