Malaria parasites encounter diverse conditions as they cycle between their vertebrate host and mosquito vector. Within these distinct environments, the parasite undergoes drastic transformations, changing both its mor...Malaria parasites encounter diverse conditions as they cycle between their vertebrate host and mosquito vector. Within these distinct environments, the parasite undergoes drastic transformations, changing both its morphology and metabolism. Plasmodium species that infect mammals must first take up residence in the liver before initiating red blood cell infection. Following penetration into hepatocytes, the parasite converts from an invasion-competent, motile, elongated sporozoite to a metabolically active, round trophozoite. Relatively little is known about the cellular events involved in sporozoite metamorphosis. Our data uncover the early cellular events associated with these transformations. We illustrate that the beginning of metamorphosis is marked by the disruption of the membrane cytoskeleton beneath the plasma membrane, which results in a protruding area around the nucleus. As this bulbous region expands, the two distal ends of the sporozoite gradually retract and disappear, leading to cell sphericalization. This shape change is associated with major interior renovations and clearance of superfluous organelles, e.g. micronemes involved in invasion. The membrane cytoskeleton is reorganized into dense lamellar arrays within the cytoplasm and is partially expulsed by converting parasites. Simultaneously, micronemes are compartmentalized into large exocytic vesicles and are then discharged into the environment. At the completion of metamorphosis, the parasites only retain organelles necessary for replication. These observations lay the groundwork for further investigations on the developmental pathways implicated in the metamorphosis of the malaria parasite.展开更多
A multicellular DCX （dc-dc transformer） using unregulated cell converters has been proposed for the environmentally friendly data centers. The high speed cell converter with the switching frequency over MHz behaves ...A multicellular DCX （dc-dc transformer） using unregulated cell converters has been proposed for the environmentally friendly data centers. The high speed cell converter with the switching frequency over MHz behaves as an ideal transformer, and this behavior solves the voltage imbalance issue in the multicellular converter topology. The analysis of the unregulated cell converter is conducted by using the state space averaging method, and the operation condition for the ideal transformer is specified. The behavior of the multicellular DCX using the high speed cell converters has been also analyzed, and the voltage imbalance issue among cell converters is discussed quantitatively. A prototype of a 19.2 kW 384 V-384 V multicellular DCX using sixty-four unregulated cell converters is fabricated and the validity of the analyses is verified.展开更多
RNA editing is a type of post-transcriptional modification that includes nucleotide insertion/deletion or conversion. Different categories of RNA editing have been widely observed in distinct RNAs from divergent organ...RNA editing is a type of post-transcriptional modification that includes nucleotide insertion/deletion or conversion. Different categories of RNA editing have been widely observed in distinct RNAs from divergent organisms. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria, playing important roles in various plant developmental processes, including organelle biogenesis, adaptation to environmental changes, and signal transduction. Numerous studies have demonstrated that a number of factors are involved in plant RNA editing, such as pentatricopeptide repeat(PPR) proteins, multiple organelle RNA editing factors(MORF, also known as RIP), organelle RNA recognition motif(ORRM) containing proteins,protoporphyrinogen IX oxidase 1(PPO1) and organelle zinc finger 1(OZ1). These factors play diverse roles in plant RNA editing due to their distinct characteristics. In this review, we discuss the functional roles of the individual editing factors and their associations in plant RNA editing.展开更多
文摘Malaria parasites encounter diverse conditions as they cycle between their vertebrate host and mosquito vector. Within these distinct environments, the parasite undergoes drastic transformations, changing both its morphology and metabolism. Plasmodium species that infect mammals must first take up residence in the liver before initiating red blood cell infection. Following penetration into hepatocytes, the parasite converts from an invasion-competent, motile, elongated sporozoite to a metabolically active, round trophozoite. Relatively little is known about the cellular events involved in sporozoite metamorphosis. Our data uncover the early cellular events associated with these transformations. We illustrate that the beginning of metamorphosis is marked by the disruption of the membrane cytoskeleton beneath the plasma membrane, which results in a protruding area around the nucleus. As this bulbous region expands, the two distal ends of the sporozoite gradually retract and disappear, leading to cell sphericalization. This shape change is associated with major interior renovations and clearance of superfluous organelles, e.g. micronemes involved in invasion. The membrane cytoskeleton is reorganized into dense lamellar arrays within the cytoplasm and is partially expulsed by converting parasites. Simultaneously, micronemes are compartmentalized into large exocytic vesicles and are then discharged into the environment. At the completion of metamorphosis, the parasites only retain organelles necessary for replication. These observations lay the groundwork for further investigations on the developmental pathways implicated in the metamorphosis of the malaria parasite.
文摘A multicellular DCX （dc-dc transformer） using unregulated cell converters has been proposed for the environmentally friendly data centers. The high speed cell converter with the switching frequency over MHz behaves as an ideal transformer, and this behavior solves the voltage imbalance issue in the multicellular converter topology. The analysis of the unregulated cell converter is conducted by using the state space averaging method, and the operation condition for the ideal transformer is specified. The behavior of the multicellular DCX using the high speed cell converters has been also analyzed, and the voltage imbalance issue among cell converters is discussed quantitatively. A prototype of a 19.2 kW 384 V-384 V multicellular DCX using sixty-four unregulated cell converters is fabricated and the validity of the analyses is verified.
基金supported by the Ministry of Science and Technology (2015CB910900)the Fok Ying-Tong Education Foundation (151021)+2 种基金the Fundamental Research Funds for the Central Universities (2017PY031 to Ping Yin)China Postdoctoral Science Foundation (2015M572163, 2017T100561)National Natural Science Foundation of China (31700203 to Junjie Yan)
文摘RNA editing is a type of post-transcriptional modification that includes nucleotide insertion/deletion or conversion. Different categories of RNA editing have been widely observed in distinct RNAs from divergent organisms. In flowering plants, RNA editing usually alters cytidine to uridine in plastids and mitochondria, playing important roles in various plant developmental processes, including organelle biogenesis, adaptation to environmental changes, and signal transduction. Numerous studies have demonstrated that a number of factors are involved in plant RNA editing, such as pentatricopeptide repeat(PPR) proteins, multiple organelle RNA editing factors(MORF, also known as RIP), organelle RNA recognition motif(ORRM) containing proteins,protoporphyrinogen IX oxidase 1(PPO1) and organelle zinc finger 1(OZ1). These factors play diverse roles in plant RNA editing due to their distinct characteristics. In this review, we discuss the functional roles of the individual editing factors and their associations in plant RNA editing.
