Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and...Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and 1,7-dimethylxanthine(17X) and assess the activity of cytochrome P-450 CYP2A6.Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method.Frequency distribution histogram was drawn by calculating the 17U/(AFMU+1X+1U+17X+17U) and then evaluated the activity of CYP2A6.Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups,the cut of point is 0.23 between fast metabolizer and intermediate type.And the cut of point is 0.15 between slow metabolizer and intermediate type.Conclusion The method is simple and rapid,suitable for the determination of metabolites of caffeine in urine.The method can be used to assay the activity of CYP2A6.展开更多
0044924 孕体的移植时机和妊娠丧失/Wilcox A J//New Eng J Med.-1999,340(23).-1796～1799 津医情0044925 母体中一种咖啡因代谢物血清1,7二甲基黄嘌呤和自发流产危险性/Kle-banoff M A//New Eng J Med.-1999,341(22).-1639～1644 津医...0044924 孕体的移植时机和妊娠丧失/Wilcox A J//New Eng J Med.-1999,340(23).-1796～1799 津医情0044925 母体中一种咖啡因代谢物血清1,7二甲基黄嘌呤和自发流产危险性/Kle-banoff M A//New Eng J Med.-1999,341(22).-1639～1644 津医情0044926 流产患者诊治中有关社会保险方面的注意点和问题点[日]/白须和裕//日产妇会志.-1999,51(5).展开更多
文摘Aim To establish a HPLC method for determining five major metabolites of caffeine in the urine,5-acetylamino-6-formylamino-3-methyluracil(AFMU),1-methylxanthine(1X),1-methyluric acid(1U),1,7-dimethyluric acid(17U) and 1,7-dimethylxanthine(17X) and assess the activity of cytochrome P-450 CYP2A6.Methods The contents of five major metabolites of caffeine in the urine were determined by RP-HPLC method.Frequency distribution histogram was drawn by calculating the 17U/(AFMU+1X+1U+17X+17U) and then evaluated the activity of CYP2A6.Results The frequency distribution histograms of CYP2A6 approximately indicated three distinct groups,the cut of point is 0.23 between fast metabolizer and intermediate type.And the cut of point is 0.15 between slow metabolizer and intermediate type.Conclusion The method is simple and rapid,suitable for the determination of metabolites of caffeine in urine.The method can be used to assay the activity of CYP2A6.
文摘0044924 孕体的移植时机和妊娠丧失/Wilcox A J//New Eng J Med.-1999,340(23).-1796～1799 津医情0044925 母体中一种咖啡因代谢物血清1,7二甲基黄嘌呤和自发流产危险性/Kle-banoff M A//New Eng J Med.-1999,341(22).-1639～1644 津医情0044926 流产患者诊治中有关社会保险方面的注意点和问题点[日]/白须和裕//日产妇会志.-1999,51(5).
