Muclear transplantation was originally designed for testing the mechanisms of cell development and differentiation.But since the report of the birth of a sheep called “Dolly”,Cloning instead of nuclear transplantati...Muclear transplantation was originally designed for testing the mechanisms of cell development and differentiation.But since the report of the birth of a sheep called “Dolly”,Cloning instead of nuclear transplantation or nuclear transfer is the most common used concept to describe the mammalian nuclear transplantation in literatures and news,which means the production of genetically identical individuals.This is the first time in mammals,a large number of cloned animals can be derived from the unlimited somatic cells or cell lines,those cells can be cultured and genetically modified in vitro to create medical important transgenes and cloning techniques also can be deployed to rescue endangered species.Cloned animals have been successfully established from donor nuclei of foetal fibroblast cells,foetal muscle cells,cumulus cells,fallopian cells,udder cells and skin epithelium cells.Human embryonic stem(ES) cells technology combining with human therapeutic cloning will be a useful tool to cure some human genetic inherent diseases.In this review,most recent exciting results were presented to address the technique improvements required and the application prospects of mammalian cloning in the near future.展开更多
The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolat...The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.展开更多
文摘Muclear transplantation was originally designed for testing the mechanisms of cell development and differentiation.But since the report of the birth of a sheep called “Dolly”,Cloning instead of nuclear transplantation or nuclear transfer is the most common used concept to describe the mammalian nuclear transplantation in literatures and news,which means the production of genetically identical individuals.This is the first time in mammals,a large number of cloned animals can be derived from the unlimited somatic cells or cell lines,those cells can be cultured and genetically modified in vitro to create medical important transgenes and cloning techniques also can be deployed to rescue endangered species.Cloned animals have been successfully established from donor nuclei of foetal fibroblast cells,foetal muscle cells,cumulus cells,fallopian cells,udder cells and skin epithelium cells.Human embryonic stem(ES) cells technology combining with human therapeutic cloning will be a useful tool to cure some human genetic inherent diseases.In this review,most recent exciting results were presented to address the technique improvements required and the application prospects of mammalian cloning in the near future.
基金the Natural Science Foundation of Shanxi Province,China (2009021038)the Doctor Initial Foundation of Shanxi Agricultural University (XB2009003)+2 种基金the Science and Technology Innovational Foundation of Shanxi Agricultural University (2009005)the Nonprofit Research Institutes,Special Foundation for Operating Expenses of Basic Research Projects of Guangxi Zhuang Autonomous Region(2060302 GXIF-2008-01)the Scientific Research and Technological Development Projects of Nanning City(200801016B)
文摘The FTZ-F1 genes encode orphan receptors of the nuclear receptor superfamily and in mammals have been found to play important roles in the proper development of the adrenal-gonadal axis and sex-determination.We isolated the homologue of FTZ-F1 in genetically improved farmed tilapia(gfFTZ-F1).The full-length cDNA was isolated from the ovary,which included an open reading frame encoding a predicted protein of 486 amino acids.Sequence,tissue distribution and phylogenic analysis of the FTZ-F1 showed that the gfFTZ-F1 belonged to SF-1/Ad4BP group and that gfFTZ-F1 transcripts were only expressed in the gonads and kidney but not in other tissues.Likewise our data suggests that the gfFTZ-F1 gene may share similar functions with other fish and mammalian counterparts,though further study is needed to make any definitive conclusions.
