Aiming at the issues of controlling the translocation speed of DNA through a solid-state nanopore and enlarging the signal-to-noise ratio of ionic current modulation, which are challenges for the application of nanopo...Aiming at the issues of controlling the translocation speed of DNA through a solid-state nanopore and enlarging the signal-to-noise ratio of ionic current modulation, which are challenges for the application of nanopore technology in DNA detection, salt concentration gradients are applied across the nanopore to investigate their influence on the DNA translocation time and signal-to-noise ratio. Experimental data demonstrates that, in symmetric concentration conditions, both the current blockade and dwell time for A-DNA translocation through a solid-state nanopore increase along with potassium chloride concentration. When the concentration in the trans chamber is decreased from 1 to 0.1 mol/L, keeping the concentration of the cis chamber at 1 mol/L, the normalized current blockade is found to be increased by one order. The increased dwell time and enhanced signal-to-noise ratio are achieved with salt gradients across the nanopore, which can improve the sensitivity when detecting DNA samples.展开更多
As the key components of nanopore-based nucleic acid sequencing systems, nanopores have drawn more and more scientific interests over these years. Although most of the early nanopore-based sequencers adopted biologica...As the key components of nanopore-based nucleic acid sequencing systems, nanopores have drawn more and more scientific interests over these years. Although most of the early nanopore-based sequencers adopted biological nanopores, solid-state nanopores have been gradually growing in popularity due to their increased robustness and durability, control over pore geometry and surface properties, as well as compatibility with the existing semiconductor and microfiuidics fabrication techniques. Besides acting as a platform for biomolecular analysis, solid-state nanopores also have great potential in many other fields such as near-field optics, nanostencil lithography and ionic logic circuitry, due to the possibility of parallel massive production. Therefore, many approaches for the fabrication of solid-state nanopores have been developed. This paper reviews the typical solid-state nanopore fabrication techniques reported to date and compares their advantages and disadvantages. The specific applications of each kind of solid-state nanopores are also summarized based on the carefully analysis of their unique morphologies and properties such as the feature size, inner structure and possibility of massive production.展开更多
基金The National Natural Science Foundation of China(No.51435003,51375092)Fundamental Research Funds for the Central Universities+1 种基金the Innovative Project for Graduate Students of Jiangsu Province(No.KYLX_0100)the Scientific Research Foundation of Graduate School of Southeast University(No.YBJJ1540)
文摘Aiming at the issues of controlling the translocation speed of DNA through a solid-state nanopore and enlarging the signal-to-noise ratio of ionic current modulation, which are challenges for the application of nanopore technology in DNA detection, salt concentration gradients are applied across the nanopore to investigate their influence on the DNA translocation time and signal-to-noise ratio. Experimental data demonstrates that, in symmetric concentration conditions, both the current blockade and dwell time for A-DNA translocation through a solid-state nanopore increase along with potassium chloride concentration. When the concentration in the trans chamber is decreased from 1 to 0.1 mol/L, keeping the concentration of the cis chamber at 1 mol/L, the normalized current blockade is found to be increased by one order. The increased dwell time and enhanced signal-to-noise ratio are achieved with salt gradients across the nanopore, which can improve the sensitivity when detecting DNA samples.
基金supported by the National Natural Science Foundation of China (91023040 and 61273061)
文摘As the key components of nanopore-based nucleic acid sequencing systems, nanopores have drawn more and more scientific interests over these years. Although most of the early nanopore-based sequencers adopted biological nanopores, solid-state nanopores have been gradually growing in popularity due to their increased robustness and durability, control over pore geometry and surface properties, as well as compatibility with the existing semiconductor and microfiuidics fabrication techniques. Besides acting as a platform for biomolecular analysis, solid-state nanopores also have great potential in many other fields such as near-field optics, nanostencil lithography and ionic logic circuitry, due to the possibility of parallel massive production. Therefore, many approaches for the fabrication of solid-state nanopores have been developed. This paper reviews the typical solid-state nanopore fabrication techniques reported to date and compares their advantages and disadvantages. The specific applications of each kind of solid-state nanopores are also summarized based on the carefully analysis of their unique morphologies and properties such as the feature size, inner structure and possibility of massive production.
