[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying bio...[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.展开更多
It is fundamental that changes in coal reservoir permeability are researched, in particular, the accurate determination of variations in the coal matrix caused by CO2 replacing CH4 at different gas saturation conditio...It is fundamental that changes in coal reservoir permeability are researched, in particular, the accurate determination of variations in the coal matrix caused by CO2 replacing CH4 at different gas saturation conditions. Based on the surface free energy, the extended Langmuir isothermal adsorption model, combined with CO2 replacing CH4 in experimental trials, and calling on the more general principles and characteristics of the field, mathematical models describing the coal matrix as it undergoes different processes such as CO2 injection and desorption were established. Combined with laboratory data about CO2 replacement under different methane saturation conditions, a law governing the variations in coal matrix CO2 replacement under different methane gas saturation conditions was obtained. The results showed that： in the injection process, the coal matrix expansion rate caused by CO2 or CH4 was exponentially increased with the CO2 pressure increase, the expansion caused by CO2 was far greater than the expansion caused by CH4 in the desorption process, the coal matrix shrinkage caused by CO2 or CH4 was exponentially increased with the pressure decrease, the shrinkage caused by CO2 was larger than the shrinkage caused by CH4 under the same pressure and different gas saturation, the total shrinkage in the desorption process in the coal matrix was greater than the total expansion in the injection process. At higher gas saturations, the total coal matrix shrinkage volume exceeded the total expansion corresponding to pressure points higher in the desorption process.展开更多
基金Supported by the Innovation Foundation For Postgraduate of Guangxi University(2008105930905D001) the Tackle Key Program in Science and Technology of Science and Technology Bureau of Guangxi Province(0815008-2-4)~~
文摘[Objective] The experiment aimed to set up a method for isolating and culturing endometrial stromal cells (BESC) and endometrial glandular epithelial cells(BEGEC) of buffalo as well as laid foundation for studying biological mechanism of embryo implantation and uterine diseases. [Method] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique were used to isolate BESC and BEGEC, then immunocytochemical method and TRYPAN-Blue assay were used to determine the purity and survival rate of isolated cells. [Result] The BESC and BEGEC were successfully isolated and cultured while immunocytochemical method and cell count method demonstrated that the purity was over 90%. The result of TRYPAN-Blue assay shown that survival rate of BESC and BEGEC was 91% and 78% respectively. [Conclusion] The enzymatic digestion method, scraping method, serial filtration and differential velocity adherent technique could isolate BESC and BEGEC with high purity.
文摘It is fundamental that changes in coal reservoir permeability are researched, in particular, the accurate determination of variations in the coal matrix caused by CO2 replacing CH4 at different gas saturation conditions. Based on the surface free energy, the extended Langmuir isothermal adsorption model, combined with CO2 replacing CH4 in experimental trials, and calling on the more general principles and characteristics of the field, mathematical models describing the coal matrix as it undergoes different processes such as CO2 injection and desorption were established. Combined with laboratory data about CO2 replacement under different methane saturation conditions, a law governing the variations in coal matrix CO2 replacement under different methane gas saturation conditions was obtained. The results showed that： in the injection process, the coal matrix expansion rate caused by CO2 or CH4 was exponentially increased with the CO2 pressure increase, the expansion caused by CO2 was far greater than the expansion caused by CH4 in the desorption process, the coal matrix shrinkage caused by CO2 or CH4 was exponentially increased with the pressure decrease, the shrinkage caused by CO2 was larger than the shrinkage caused by CH4 under the same pressure and different gas saturation, the total shrinkage in the desorption process in the coal matrix was greater than the total expansion in the injection process. At higher gas saturations, the total coal matrix shrinkage volume exceeded the total expansion corresponding to pressure points higher in the desorption process.
