With designed specific primers based on Potato leafroll virus(PLRV) coat protein(CP) gene sequence,one PCR fragment(about 0.6 kb) was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the tota...With designed specific primers based on Potato leafroll virus(PLRV) coat protein(CP) gene sequence,one PCR fragment(about 0.6 kb) was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the total RNA as template extracted from virus infected potato plant collected in Zhaotong,Yunnan province.The fragment was cloned into pGEM-T easy vector and sequenced.The sequence was compared with that of homologous gene of other PLRV isolates.The results showed it had high homology with the other isolates(the highest homology reached 99.2% of nucleic acid).Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.展开更多
文摘With designed specific primers based on Potato leafroll virus(PLRV) coat protein(CP) gene sequence,one PCR fragment(about 0.6 kb) was amplified by reverse transcription polymerase chain reaction(RT-PCR) using the total RNA as template extracted from virus infected potato plant collected in Zhaotong,Yunnan province.The fragment was cloned into pGEM-T easy vector and sequenced.The sequence was compared with that of homologous gene of other PLRV isolates.The results showed it had high homology with the other isolates(the highest homology reached 99.2% of nucleic acid).Based on CP amino acid sequence the phylogenic tree of PLRV was established and the isolates were clustered into many groups.
