During the course of mungbean (Phaseolus radiatus L.) germination, the rate of ethylene production and the activity of ACC synthase (1_aminocyclopropane_1_carboxylic acid synthase, EC4.4.1.4) began to increase in the ...During the course of mungbean (Phaseolus radiatus L.) germination, the rate of ethylene production and the activity of ACC synthase (1_aminocyclopropane_1_carboxylic acid synthase, EC4.4.1.4) began to increase in the 5th day of germination, and reached its peak in the 10th day and then decreased. The ethylene production and the activity of ACC synthase were obviously promoted by 10 μmol/L exogenous IAA (indole_3_acetic acid). The production of superoxide radical (O -· 2) and hydrogen peroxide (H 2O 2) were also promoted by exogenous IAA, suggesting that there was some relationship between active oxygen production and the activity of ACC synthase induced by exogenous IAA. The production of ethylene and the activity of ACC synthase increased dramatically when the seedlings were treated with exogenous O -· 2, whereas the exogenous H 2O 2 had no effects on the production of ethylene and the activity of ACC synthase. Exogenous SOD (superoxide dismutase, one scavenger of O -· 2) could inhibit the production of ethylene and the activity of ACC synthase, but exogenous CAT (catalase) could not. So it was possible that IAA would stimulate the activity of ACC synthase by inducing the production of O -· 2 in germinating mungbean seedlings, and this might be one of the regulating mechanism of ethylene synthesis in higher plants; the production of H 2O 2 induced by IAA was not the cause of the increase of the activity of ACC synthase and the production of ethylene.展开更多
以来自青藏高原高寒草地野生老芒麦(Elymus sibiricus L.)种子为试验材料,采用营养砂培法,研究了根部施用不同体积(0,3,5,7,10 mL)磷素(NaH2PO4,200 g·L-1)溶液搭配叶面喷施不同体积(10,7,5,3,0 mL)外源激素6-苄氨基嘌呤(6-BA,20 m...以来自青藏高原高寒草地野生老芒麦(Elymus sibiricus L.)种子为试验材料,采用营养砂培法,研究了根部施用不同体积(0,3,5,7,10 mL)磷素(NaH2PO4,200 g·L-1)溶液搭配叶面喷施不同体积(10,7,5,3,0 mL)外源激素6-苄氨基嘌呤(6-BA,20 mg·L-1)和吲哚乙酸(IAA,30 mg·L-1)对老芒麦生长特性及营养品质的影响,旨在为外源激素和磷素的互作效应以及老芒麦人工草地建植技术提供理论依据。试验结果表明:在7 mL NaH2PO4+3 mL 6-BA(PB7+3)和7 mL NaH2PO4+3 mL IAA(PI7+3)处理下,地上干重较对照分别增加33%和26%;粗蛋白含量在10 mL NaH2PO4+0 mL 6-BA(PB10+0)、10 mL NaH2PO4+0 mL IAA(PI10+0)处理下较对照各增加了24%;酸性洗涤纤维(ADF)含量在5 mL NaH2PO4+5 mL IAA(PI5+5)处理下较对照降低了4%,老芒麦磷含量在5 mL NaH2PO4+5 mL 6-BA(PB5+5)和3 mL NaH2PO4+7 mL IAA(PI3+7)处理下较对照分别增加了46%和50%。经灰色关联度对地上干重和营养品质综合分析表明,P素与6-BA和IAA配施可以提高老芒麦生长特性和营养品质,其中在5 mL NaH2PO4+5 mL 6-BA(PB5+5)处理下配施效果较佳,本试验结果可为外源激素和磷素的互作效应以及老芒麦人工草地建植技术提供理论依据。展开更多
文摘During the course of mungbean (Phaseolus radiatus L.) germination, the rate of ethylene production and the activity of ACC synthase (1_aminocyclopropane_1_carboxylic acid synthase, EC4.4.1.4) began to increase in the 5th day of germination, and reached its peak in the 10th day and then decreased. The ethylene production and the activity of ACC synthase were obviously promoted by 10 μmol/L exogenous IAA (indole_3_acetic acid). The production of superoxide radical (O -· 2) and hydrogen peroxide (H 2O 2) were also promoted by exogenous IAA, suggesting that there was some relationship between active oxygen production and the activity of ACC synthase induced by exogenous IAA. The production of ethylene and the activity of ACC synthase increased dramatically when the seedlings were treated with exogenous O -· 2, whereas the exogenous H 2O 2 had no effects on the production of ethylene and the activity of ACC synthase. Exogenous SOD (superoxide dismutase, one scavenger of O -· 2) could inhibit the production of ethylene and the activity of ACC synthase, but exogenous CAT (catalase) could not. So it was possible that IAA would stimulate the activity of ACC synthase by inducing the production of O -· 2 in germinating mungbean seedlings, and this might be one of the regulating mechanism of ethylene synthesis in higher plants; the production of H 2O 2 induced by IAA was not the cause of the increase of the activity of ACC synthase and the production of ethylene.
文摘以来自青藏高原高寒草地野生老芒麦(Elymus sibiricus L.)种子为试验材料,采用营养砂培法,研究了根部施用不同体积(0,3,5,7,10 mL)磷素(NaH2PO4,200 g·L-1)溶液搭配叶面喷施不同体积(10,7,5,3,0 mL)外源激素6-苄氨基嘌呤(6-BA,20 mg·L-1)和吲哚乙酸(IAA,30 mg·L-1)对老芒麦生长特性及营养品质的影响,旨在为外源激素和磷素的互作效应以及老芒麦人工草地建植技术提供理论依据。试验结果表明:在7 mL NaH2PO4+3 mL 6-BA(PB7+3)和7 mL NaH2PO4+3 mL IAA(PI7+3)处理下,地上干重较对照分别增加33%和26%;粗蛋白含量在10 mL NaH2PO4+0 mL 6-BA(PB10+0)、10 mL NaH2PO4+0 mL IAA(PI10+0)处理下较对照各增加了24%;酸性洗涤纤维(ADF)含量在5 mL NaH2PO4+5 mL IAA(PI5+5)处理下较对照降低了4%,老芒麦磷含量在5 mL NaH2PO4+5 mL 6-BA(PB5+5)和3 mL NaH2PO4+7 mL IAA(PI3+7)处理下较对照分别增加了46%和50%。经灰色关联度对地上干重和营养品质综合分析表明,P素与6-BA和IAA配施可以提高老芒麦生长特性和营养品质,其中在5 mL NaH2PO4+5 mL 6-BA(PB5+5)处理下配施效果较佳,本试验结果可为外源激素和磷素的互作效应以及老芒麦人工草地建植技术提供理论依据。
