【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture c...【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.展开更多
[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,...[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,and liquid ratio on the extraction rate of polysaccharides from chrysanthemum were studied.The optimum operation condition by microwave extraction method was determined and was compared with that by traditional extraction method.[Result] The optimum extraction conditions were:800 W,1:15(solid-liquid ratio),and 15 min.The polysaccharides extraction yield under such optimum condition was 5.59%.The extraction yield of impregnation was generally about 2.73%.[Conclusion] Compared with the traditional extraction method,the microwave extraction was simple,convenient,energy saving,and efficient,and was suitable for industrial extraction of polysaccharide.展开更多
[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and thr...[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and throe-levels was carried out for optimizing the extraction process of polysacchafides from Tegillarca granosa. A central composite des（gn including independent variables, such as extraction temperature （A）, extraction time （B）, and ethanol concentration （C） was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysacchadde yield. [ Result] The independent variable with the largest effect on response was ethanol concentration （C）. The optimum extraction conditions were found to be extraction temperature 69.6℃, extraction time 6.2 h, and ethanol concen- tration of 78% （V/V）, respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1. 635%. [ Coaclusioa] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.展开更多
文摘【Objective】To optimize the steam explosion process condition for extracting polysaccharides from Pseudostellaria heterophylla.【Method】The effects of steam pressure,pressure-maintaining time and material moisture content on the extraction of polysaccharides from Pseudostellaria heterophylla were studied by response surface methodology based on Box-Behnken design.【Result】The findings showed that each factor could significantly affect the test index,and the optimum condition was as follows:steam pressure 1.50 Mpa,pressuremaintaining time 46 s and material moisture content 46%.Under this condition,the verified experimental value of polysaccharides from Pseudostellaria heterophylla was 39.32%,indicating a relative standard deviation of 2.73%from the predictive value.Meanwhile,scanning electron microcopy(SEM)images showed that the surface physical structure of Pseudostellaria heterophylla was irregularly broken and cracked,which means the physical structure of Pseudostellaria heterophylla was changed and destroyed at the cellular level.【Conclusion】This experiment provides a new approach for the extraction of polysaccharides from Pseudostellaria heterophylla,as well as a reference for the resource utilization of Pseudostellaria heterophylla.
基金Supported by Doctoral Initial Funding of Henan University of TCM(BSJJ2009-34)
文摘[Objective] The aim was to study the optimum extraction process of polysaccharide from chrysanthemum.[Method] By dint of orthogonal and single-factor experiments,the influences of microwave efficiency,extraction time,and liquid ratio on the extraction rate of polysaccharides from chrysanthemum were studied.The optimum operation condition by microwave extraction method was determined and was compared with that by traditional extraction method.[Result] The optimum extraction conditions were:800 W,1:15(solid-liquid ratio),and 15 min.The polysaccharides extraction yield under such optimum condition was 5.59%.The extraction yield of impregnation was generally about 2.73%.[Conclusion] Compared with the traditional extraction method,the microwave extraction was simple,convenient,energy saving,and efficient,and was suitable for industrial extraction of polysaccharide.
基金Supported by Key Scientific Research Program of Wannan MedicalCollege ( WK2012Z208)
文摘[ Objective] The aim was to use response surface methodology to determine optimum conditions for extraction of polysaccharides from Tegillarca granosa. [ Method] Response surface methodology with three-factors and throe-levels was carried out for optimizing the extraction process of polysacchafides from Tegillarca granosa. A central composite des（gn including independent variables, such as extraction temperature （A）, extraction time （B）, and ethanol concentration （C） was obtained through Box-Benhnken central combination design. Selected response which evaluates the extraction process was polysacchadde yield. [ Result] The independent variable with the largest effect on response was ethanol concentration （C）. The optimum extraction conditions were found to be extraction temperature 69.6℃, extraction time 6.2 h, and ethanol concen- tration of 78% （V/V）, respectively. Under these conditions, the extraction efficiency of polysaccharide can increase to 1. 635%. [ Coaclusioa] Study on the extraction of polysaccharides from Tegillarca granosa could provide certain theoretical direction for extracting polysaccharides from Tegillarca granosa on a large scale.
