利用热水水解法制备大菱鲆鱼皮胶原蛋白。以制备的胶原蛋白为原料,利用复合蛋白酶酶解法制备大菱鲆鱼皮多肽液,并利用单因素和正交试验对复合蛋白酶酶解大菱鲆鱼皮胶原蛋白的工艺进行优化,并对得到的大菱鲆鱼皮多肽液的抗运动疲劳作用...利用热水水解法制备大菱鲆鱼皮胶原蛋白。以制备的胶原蛋白为原料,利用复合蛋白酶酶解法制备大菱鲆鱼皮多肽液,并利用单因素和正交试验对复合蛋白酶酶解大菱鲆鱼皮胶原蛋白的工艺进行优化,并对得到的大菱鲆鱼皮多肽液的抗运动疲劳作用进行研究。结果表明,复合蛋白酶酶解法制备大菱鲆鱼皮多肽液的最佳酶解工艺为:温度50℃,p H 6.5,酶用量0.08%,酶解时间8 h。在此工艺条件下大菱鲆鱼皮胶原蛋白的水解度为68.35%。小鼠负重游泳实验和抗疲劳生化指标结果表明大菱鲆鱼皮多肽液具有良好的抗运动疲劳能力。展开更多
AIM： To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor i （VPCAPrR） and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps. METHODS： The expr...AIM： To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor i （VPCAPrR） and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps. METHODS： The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone, 8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： The VPCAP2-R mRNA expression level in the control group （1.09±0.58） was lower than that in the gallbladder polyp group （1.64±0.56） and the gallstone group （1.55±0.45） （P〈0.05） while the VPCAP1-R mRNA expression level in the control group （1.15 ±0.23） was not apparently different from that in the gallbladder polyp group （1.28±0.56） and the gallstone group （1.27± 0.38）. CONCLUSION： The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.展开更多
Densities of aqueous solutions of eight amino acids, glycine, L-alanine, L-valine, L-isoleucine, L-serine, L-threonine, L-arginine and L-phenylalanine, are measured as a function of amino acid concentration from 293.1...Densities of aqueous solutions of eight amino acids, glycine, L-alanine, L-valine, L-isoleucine, L-serine, L-threonine, L-arginine and L-phenylalanine, are measured as a function of amino acid concentration from 293.15K to 333.15K. These data are used to calculate the apparent molar volume Vφ and infinite dilution apparent molar volume Vφo (partial molar volume). Data of five amino acids are used to correlate partial molar volume Vφo usinggroup contribution method to estimate the contributions of the zwitterionic end groups (NH3+,COO-) and CH2 group, OH group, CNHNHNH2 group and C6H5(phenyl) group of amino acids. The results show that Vφo values for all kinds of groups of amino acids studied increase with increase of temperature except those for CH2 group, which are almost constant within the studied temperature range. Data of other amino acids, L-valine, L-isoleucine and L-threonine, are chosen for comparison with the predicted partial molar volume Vφo using the group additivity parameters obtained. The results confirm that this group additivity method has excellent predictive utility.展开更多
Glutathione S-transferases(GSTs)are important drug-metabolizing enzymes that catalyze the binding of glutathione(GSH)to electrophilic substances.GST has genetic polymorphism,and the enzyme activity of GST affects the ...Glutathione S-transferases(GSTs)are important drug-metabolizing enzymes that catalyze the binding of glutathione(GSH)to electrophilic substances.GST has genetic polymorphism,and the enzyme activity of GST affects the metabolism of certain drugs in vivo.In the present day,we investigated the GST enzyme activity and GSTA1 gene polymorphism in 170 patients with hematological diseases and explored their relationship.The GSTA1 gene polymorphism of the patient was analyzed by PCR-restriction fragment length polymorphism(PCR-RFLP)technique,and the base sequences of the four mutation sites(-631,-567,-69,and-52)in the promoter region were determined by DNA-Sequencer.The patient’s GST enzyme activity was calculated by measuring the rate at which it catalyzed the reaction between 1-chloro-2,4-dinitrobenzene(CDNB)and GSH.The average GST enzyme activities of males and females were 5.20±0.13 and 5.17±0.12 nmol/min/mL,respectively,and the difference was not significant(P=0.91).The frequencies of genotypes GSTA1*A*A(wild genotype),GSTA1*A*B(heterozygous genotype),and GSTA1*B*B(homozygous mutant genotype)were 75.3%,22.9%,and 1.8%,respectively.Alleles GSTA1*A and*B were distributed at 86.8%and 13.2%,respectively.The genotype frequency distribution between males and females was no significant difference by Pearson’s chi-square test(P=0.743).The average GST activity of the heterozygous mutant genotype(4.83±0.76 nmol/min/mL)was lower than the wild genotype(5.34±1.26 nmol/min/mL,P=0.018),and higher than that of the homozygous mutant genotype(3.32±0.07 nmol/min/mL,P=0.022).These findings might help us improve the individualized treatment of patients with hematological diseases in the future and promote the development of precision medicine for blood diseases.展开更多
文摘利用热水水解法制备大菱鲆鱼皮胶原蛋白。以制备的胶原蛋白为原料,利用复合蛋白酶酶解法制备大菱鲆鱼皮多肽液,并利用单因素和正交试验对复合蛋白酶酶解大菱鲆鱼皮胶原蛋白的工艺进行优化,并对得到的大菱鲆鱼皮多肽液的抗运动疲劳作用进行研究。结果表明,复合蛋白酶酶解法制备大菱鲆鱼皮多肽液的最佳酶解工艺为:温度50℃,p H 6.5,酶用量0.08%,酶解时间8 h。在此工艺条件下大菱鲆鱼皮胶原蛋白的水解度为68.35%。小鼠负重游泳实验和抗疲劳生化指标结果表明大菱鲆鱼皮多肽液具有良好的抗运动疲劳能力。
文摘AIM： To detect the expression of pituitary adenylate cyclase-activating polypeptide receptor i （VPCAPrR） and VPCAP2-R mRNA in gallbladder tissues of patients with gallstone or gallbladder polyps. METHODS： The expression of VPCAP1-R and VPCAP2-R mRNA in gallbladder tissues was detected in 25 patients with gallstone, 8 patients with gallbladder polyps and 7 donors of liver transplantation by reverse transcription polymerase chain reaction （RT-PCR）. RESULTS： The VPCAP2-R mRNA expression level in the control group （1.09±0.58） was lower than that in the gallbladder polyp group （1.64±0.56） and the gallstone group （1.55±0.45） （P〈0.05） while the VPCAP1-R mRNA expression level in the control group （1.15 ±0.23） was not apparently different from that in the gallbladder polyp group （1.28±0.56） and the gallstone group （1.27± 0.38）. CONCLUSION： The abnormal expression of VPCAP2-R mRNA in gallbladder tissue may play a role in the formation of gallbladder stone and gallbladder polyps.
基金the Educational Department Doctor Foundation of China (No. 2000005608).
文摘Densities of aqueous solutions of eight amino acids, glycine, L-alanine, L-valine, L-isoleucine, L-serine, L-threonine, L-arginine and L-phenylalanine, are measured as a function of amino acid concentration from 293.15K to 333.15K. These data are used to calculate the apparent molar volume Vφ and infinite dilution apparent molar volume Vφo (partial molar volume). Data of five amino acids are used to correlate partial molar volume Vφo usinggroup contribution method to estimate the contributions of the zwitterionic end groups (NH3+,COO-) and CH2 group, OH group, CNHNHNH2 group and C6H5(phenyl) group of amino acids. The results show that Vφo values for all kinds of groups of amino acids studied increase with increase of temperature except those for CH2 group, which are almost constant within the studied temperature range. Data of other amino acids, L-valine, L-isoleucine and L-threonine, are chosen for comparison with the predicted partial molar volume Vφo using the group additivity parameters obtained. The results confirm that this group additivity method has excellent predictive utility.
文摘Glutathione S-transferases(GSTs)are important drug-metabolizing enzymes that catalyze the binding of glutathione(GSH)to electrophilic substances.GST has genetic polymorphism,and the enzyme activity of GST affects the metabolism of certain drugs in vivo.In the present day,we investigated the GST enzyme activity and GSTA1 gene polymorphism in 170 patients with hematological diseases and explored their relationship.The GSTA1 gene polymorphism of the patient was analyzed by PCR-restriction fragment length polymorphism(PCR-RFLP)technique,and the base sequences of the four mutation sites(-631,-567,-69,and-52)in the promoter region were determined by DNA-Sequencer.The patient’s GST enzyme activity was calculated by measuring the rate at which it catalyzed the reaction between 1-chloro-2,4-dinitrobenzene(CDNB)and GSH.The average GST enzyme activities of males and females were 5.20±0.13 and 5.17±0.12 nmol/min/mL,respectively,and the difference was not significant(P=0.91).The frequencies of genotypes GSTA1*A*A(wild genotype),GSTA1*A*B(heterozygous genotype),and GSTA1*B*B(homozygous mutant genotype)were 75.3%,22.9%,and 1.8%,respectively.Alleles GSTA1*A and*B were distributed at 86.8%and 13.2%,respectively.The genotype frequency distribution between males and females was no significant difference by Pearson’s chi-square test(P=0.743).The average GST activity of the heterozygous mutant genotype(4.83±0.76 nmol/min/mL)was lower than the wild genotype(5.34±1.26 nmol/min/mL,P=0.018),and higher than that of the homozygous mutant genotype(3.32±0.07 nmol/min/mL,P=0.022).These findings might help us improve the individualized treatment of patients with hematological diseases in the future and promote the development of precision medicine for blood diseases.
