Objective: Genetic as well as hormonal factors are known to influence the development and clinical course of endometriosis. We aimed to investigate the association among 10 single nucleotide polymorphisms (SNPs) invol...Objective: Genetic as well as hormonal factors are known to influence the development and clinical course of endometriosis. We aimed to investigate the association among 10 single nucleotide polymorphisms (SNPs) involved in the estrogen metabolism and endometriosis and to develop a multiple genetic model. METHODS: In a case-control study, we investigated the genotype frequencies of 10 estrogen metabolizing SNPs in 32 patients with endometriosis and 790 healthy controls using sequencing-on-chip-technology with solid-phase polymerase chain reaction on oligonucleotide microarrays: catechol-O-methy-ltransferase, Val158Met G->A, 17-β-hydroxysteroid dehydrogenase type 1 (HSD17), vIV A->C, cytochrome P450 (CYP), 17 A2 allele T->C, CYP1A1 Mspl RFLP T->C, CYP1A1 Ile462ValA->G, CYP19 Arg264-Cys C->T, CYP19 C1558T C->T, CYP 1B1 Leu432Val, CYP1B1 Asn453Ser, and estrogen receptor alpha. IVS1-401>C. Associations and 2-way interaction models between SNPs were calculated by stepwise logistic regression models. RESULTS: In a univariate model, HSD17 vIV A->C was associated with a significantly increased risk of endometriosis (P = .004; odds ratio 3.9, 95%confidence interval 1.6-9.8). When all 2-way interactions of investigated SNPs were ascertained, no significant interactions among SNPs were observed. In a multivariate model, HSD17 vIV A->C was also significantly associated with endometriosis (P = .002). CONCLUSION: We present data on multiple SNPs in patients with endometriosis indicating an association between HSD17 gene variation and the disease. Although not able to demonstrate interaction models of SNPs, we provide evidence of HSD17 vIV A->C as a low penetrance genetic marker of endometriosis.展开更多
1950年,张明觉先生研究家兔胚胎移植时发现了胚龄与子宫内膜同步化的重要性,并首次提及子宫内膜容受性(endometrial receptivity,ER),指在特定时间子宫内膜允许胚胎接触、定位、黏附、植入的能力,而这一特定时间段被称为“种植窗(window...1950年,张明觉先生研究家兔胚胎移植时发现了胚龄与子宫内膜同步化的重要性,并首次提及子宫内膜容受性(endometrial receptivity,ER),指在特定时间子宫内膜允许胚胎接触、定位、黏附、植入的能力,而这一特定时间段被称为“种植窗(window of implantation,WOI)”,WOI一般在正常月经周期第20~24天或LH峰7~10天[1]。近年来,ER越来越成为生殖医学专业的研究重点,但其评估和治疗方法研究进展甚微。展开更多
本文报道了1例患者2年间接受6次体外受精胚胎移植(invitro fertilization and embryo transfer,IVF-ET)均失败,后接受中药周期序贯疗法调理后自然怀孕的病案。经调而后能孕,根据月经周期气血阴阳的变化调理月经以助孕,卵泡期滋阴为主用...本文报道了1例患者2年间接受6次体外受精胚胎移植(invitro fertilization and embryo transfer,IVF-ET)均失败,后接受中药周期序贯疗法调理后自然怀孕的病案。经调而后能孕,根据月经周期气血阴阳的变化调理月经以助孕,卵泡期滋阴为主用育胞汤,排卵期滋阴稍加温阳活血用促排卵汤,黄体期温阳为主用两固汤,月经期活血祛瘀为主用养血调经汤,对患者进行针对性治疗,7个月共就诊7次,开出3个周期药物进行序贯治疗。该案例的成功提示中药周期序贯疗法能促进卵巢功能,改善子宫内膜容受性,提升自然妊娠率,同时辅助调畅情志,对反复不明原因IVF-ET失败患者可起到良性调节作用。展开更多
文摘Objective: Genetic as well as hormonal factors are known to influence the development and clinical course of endometriosis. We aimed to investigate the association among 10 single nucleotide polymorphisms (SNPs) involved in the estrogen metabolism and endometriosis and to develop a multiple genetic model. METHODS: In a case-control study, we investigated the genotype frequencies of 10 estrogen metabolizing SNPs in 32 patients with endometriosis and 790 healthy controls using sequencing-on-chip-technology with solid-phase polymerase chain reaction on oligonucleotide microarrays: catechol-O-methy-ltransferase, Val158Met G->A, 17-β-hydroxysteroid dehydrogenase type 1 (HSD17), vIV A->C, cytochrome P450 (CYP), 17 A2 allele T->C, CYP1A1 Mspl RFLP T->C, CYP1A1 Ile462ValA->G, CYP19 Arg264-Cys C->T, CYP19 C1558T C->T, CYP 1B1 Leu432Val, CYP1B1 Asn453Ser, and estrogen receptor alpha. IVS1-401>C. Associations and 2-way interaction models between SNPs were calculated by stepwise logistic regression models. RESULTS: In a univariate model, HSD17 vIV A->C was associated with a significantly increased risk of endometriosis (P = .004; odds ratio 3.9, 95%confidence interval 1.6-9.8). When all 2-way interactions of investigated SNPs were ascertained, no significant interactions among SNPs were observed. In a multivariate model, HSD17 vIV A->C was also significantly associated with endometriosis (P = .002). CONCLUSION: We present data on multiple SNPs in patients with endometriosis indicating an association between HSD17 gene variation and the disease. Although not able to demonstrate interaction models of SNPs, we provide evidence of HSD17 vIV A->C as a low penetrance genetic marker of endometriosis.
文摘1950年,张明觉先生研究家兔胚胎移植时发现了胚龄与子宫内膜同步化的重要性,并首次提及子宫内膜容受性(endometrial receptivity,ER),指在特定时间子宫内膜允许胚胎接触、定位、黏附、植入的能力,而这一特定时间段被称为“种植窗(window of implantation,WOI)”,WOI一般在正常月经周期第20~24天或LH峰7~10天[1]。近年来,ER越来越成为生殖医学专业的研究重点,但其评估和治疗方法研究进展甚微。
文摘本文报道了1例患者2年间接受6次体外受精胚胎移植(invitro fertilization and embryo transfer,IVF-ET)均失败,后接受中药周期序贯疗法调理后自然怀孕的病案。经调而后能孕,根据月经周期气血阴阳的变化调理月经以助孕,卵泡期滋阴为主用育胞汤,排卵期滋阴稍加温阳活血用促排卵汤,黄体期温阳为主用两固汤,月经期活血祛瘀为主用养血调经汤,对患者进行针对性治疗,7个月共就诊7次,开出3个周期药物进行序贯治疗。该案例的成功提示中药周期序贯疗法能促进卵巢功能,改善子宫内膜容受性,提升自然妊娠率,同时辅助调畅情志,对反复不明原因IVF-ET失败患者可起到良性调节作用。
