子宫内膜异位症(endometriosis,EMs)是全球女性健康面临的常见挑战。近年来,铁死亡作为一种新兴的细胞程序性死亡方式,在EMs研究中备受瞩目。在疾病机制方面,铁死亡通过铁过载诱导,推动卵巢EMs的纤维化,同时通过调控多条信号通路如p38...子宫内膜异位症(endometriosis,EMs)是全球女性健康面临的常见挑战。近年来,铁死亡作为一种新兴的细胞程序性死亡方式,在EMs研究中备受瞩目。在疾病机制方面,铁死亡通过铁过载诱导,推动卵巢EMs的纤维化,同时通过调控多条信号通路如p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38 MAPK)/信号转导子和转录激活子6(signal transducer and activator of transcription 6,STAT6),促进血管生成、自噬等,加剧病情进展。在不孕问题上,铁死亡影响胚胎发育和卵巢功能,显著降低生育能力。诊断方面,铁死亡相关基因高表达为EMs的早期诊断提供了潜在的标志物,有效区分患者和健康个体,具有重要的临床应用价值。治疗策略上,Erastin等非天然化合物及白藜芦醇、熊果酸和汉黄芩素等天然化合物,展现出潜在的治疗效果,可缓解EMs的病理进程和相关症状。因此,本文通过综述铁死亡在EMs发病机制、不孕问题、诊断和治疗中的多重作用,全面阐述了铁死亡在子宫内膜异位症中的关键作用,探索其作为诊断标志物和治疗靶点的潜力,为EMs的管理提供新的理论支持和治疗策略。展开更多
目的:研究剖宫产术后腹壁子宫内膜异位症(AWE)的临床特征,以了解其相关特点。方法:对滨州医学院附属医院在2018年1月至2024年5月期间收治的134例经组织病理证实为剖宫产术后腹壁子宫内膜异位症患者的临床资料进行回顾性分析。根据腹壁...目的:研究剖宫产术后腹壁子宫内膜异位症(AWE)的临床特征,以了解其相关特点。方法:对滨州医学院附属医院在2018年1月至2024年5月期间收治的134例经组织病理证实为剖宫产术后腹壁子宫内膜异位症患者的临床资料进行回顾性分析。根据腹壁子宫内膜异位症病灶的侵袭深度及范围,将其分为皮下脂肪型、鞘膜型和腹膜型三种类型。按照内异症病灶数量分为单发型、多发型。比较腹壁子宫内膜异位症各个分型之间的临床特点及差异。结果:根据术中探查内异症病灶数分型,单发型122例(91%),多发型12例(9%)。与单发型相比,多发型AWE的手术时间长(P P P = 0.035),手术时间更长(P P P P P P Objective: To investigate the clinical characteristics of abdominal wall endometriosis (AWE) after cesarean section in order to understand its related characteristics. Methods: The clinical data of 134 patients with abdominal wall endometriosis confirmed by histopathology admitted to the Affiliated Hospital of Binzhou Medical University from January 2018 to May 2024 were retrospectively analyzed. According to the invasion depth and scope of abdominal endometriosis, the lesions were classified into subcutaneous lipomatosis, vaginalis, and peritoneum. Based on the number of endometriosis lesions, it can be divided into single types and multiple types. To compare the clinical features and differences among different types of abdominal endometriosis. Results: According to the number of intraoperative investigations, there were 122 cases (91%) of single type and 12 cases (9%) of multiple type. Compared with single-style AWE, multi-style AWE had longer operation time (P P P = 0.035), longer operation time (P P P P P P < 0.01).展开更多
目的:基于网络药理学的方法探讨中药黄芩–夏枯草–金银花治疗子宫内膜异位症的作用机制。方法:使用中药系统药理学数据库与分析平台(TCMSP)搜索黄芩、夏枯草及金银花的有效活性成分及作用靶点,通过GeneCards、OMIM和DisGeNet数据库筛...目的:基于网络药理学的方法探讨中药黄芩–夏枯草–金银花治疗子宫内膜异位症的作用机制。方法:使用中药系统药理学数据库与分析平台(TCMSP)搜索黄芩、夏枯草及金银花的有效活性成分及作用靶点,通过GeneCards、OMIM和DisGeNet数据库筛选子宫内膜异位症疾病的相关靶点。使用Venny 2.1在线工具构建黄芩–夏枯草–金银花作用靶点与子宫内膜异位症疾病靶点韦恩图,获得交集靶点基因。利用Cytoscape 3.7.1软件对药物–成分–交集靶点网络进行可视化构建,同时将获得的交集靶点基因上传至STRING网站进行蛋白质–蛋白质相互作用分析,使用Metascape在线分析平台对交集靶点进行GO富集分析与KEGG通路富集分析。结果:筛选获得黄芩–夏枯草–金银花的有效活性成分共52个,黄芩–夏枯草–金银花与子宫内膜异位症交集靶点有140个,相关重要靶点包括AKT1、TNF、IL6、TP53、IL1B等。GO富集分析结果显示涉及1878个生物过程,76个细胞组分,196个分子功能。KEGG通路富集获得205条相关信号通路,主要涉及的通路有PI3K-Akt信号通路、IL-17信号通路、TNF信号通路等。结论:黄芩–夏枯草–金银花可能通过多靶点、多通路发挥治疗子宫内膜异位症的作用。Objective: To explore the mechanism of action of traditional Chinese medicine Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos in the treatment of endometriosis based on network pharmacology. Methods: The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was used to search for effective active ingredients and targets of Scutellariae Radix, Spica Prunellae, Lonicerae Japonicae Flos, and related targets for endometriosis diseases were screened through GeneCards, OMIM and DisGeNet databases. Use the Venny 2.1 online tool to construct a Venn diagram of Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos action targets and endometriosis disease targets, and obtain intersection target genes. Cytoscape 3.7.1 software was used to visually construct the drug-component-intersection target network, and the obtained intersection target genes were uploaded to the STRING website for protein-protein interaction analysis. The Metascape online analysis platform was used to perform GO enrichment analysis and KEGG pathway enrichment analysis on the intersection targets. Results: A total of 52 effective active ingredients of Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos were screened, and there were 140 intersection targets between Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos and endometriosis. Related important targets include AKT1, TNF, IL6, TP53, IL1B, etc. GO enrichment analysis results showed that 1878 biological processes, 76 cellular components, and 196 molecular functions were involved. KEGG pathway enrichment obtained 205 related signaling pathways, the main pathways involved include the PI3K-Akt signaling pathway, IL-17 signaling pathway, TNF signaling pathway, etc. Conclusion: Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos may play a role in treating endometriosis through multiple targets and multiple pathways.展开更多
文摘子宫内膜异位症(endometriosis,EMs)是全球女性健康面临的常见挑战。近年来,铁死亡作为一种新兴的细胞程序性死亡方式,在EMs研究中备受瞩目。在疾病机制方面,铁死亡通过铁过载诱导,推动卵巢EMs的纤维化,同时通过调控多条信号通路如p38丝裂原活化蛋白激酶(p38 mitogen-activated protein kinase,p38 MAPK)/信号转导子和转录激活子6(signal transducer and activator of transcription 6,STAT6),促进血管生成、自噬等,加剧病情进展。在不孕问题上,铁死亡影响胚胎发育和卵巢功能,显著降低生育能力。诊断方面,铁死亡相关基因高表达为EMs的早期诊断提供了潜在的标志物,有效区分患者和健康个体,具有重要的临床应用价值。治疗策略上,Erastin等非天然化合物及白藜芦醇、熊果酸和汉黄芩素等天然化合物,展现出潜在的治疗效果,可缓解EMs的病理进程和相关症状。因此,本文通过综述铁死亡在EMs发病机制、不孕问题、诊断和治疗中的多重作用,全面阐述了铁死亡在子宫内膜异位症中的关键作用,探索其作为诊断标志物和治疗靶点的潜力,为EMs的管理提供新的理论支持和治疗策略。
文摘目的:研究剖宫产术后腹壁子宫内膜异位症(AWE)的临床特征,以了解其相关特点。方法:对滨州医学院附属医院在2018年1月至2024年5月期间收治的134例经组织病理证实为剖宫产术后腹壁子宫内膜异位症患者的临床资料进行回顾性分析。根据腹壁子宫内膜异位症病灶的侵袭深度及范围,将其分为皮下脂肪型、鞘膜型和腹膜型三种类型。按照内异症病灶数量分为单发型、多发型。比较腹壁子宫内膜异位症各个分型之间的临床特点及差异。结果:根据术中探查内异症病灶数分型,单发型122例(91%),多发型12例(9%)。与单发型相比,多发型AWE的手术时间长(P P P = 0.035),手术时间更长(P P P P P P Objective: To investigate the clinical characteristics of abdominal wall endometriosis (AWE) after cesarean section in order to understand its related characteristics. Methods: The clinical data of 134 patients with abdominal wall endometriosis confirmed by histopathology admitted to the Affiliated Hospital of Binzhou Medical University from January 2018 to May 2024 were retrospectively analyzed. According to the invasion depth and scope of abdominal endometriosis, the lesions were classified into subcutaneous lipomatosis, vaginalis, and peritoneum. Based on the number of endometriosis lesions, it can be divided into single types and multiple types. To compare the clinical features and differences among different types of abdominal endometriosis. Results: According to the number of intraoperative investigations, there were 122 cases (91%) of single type and 12 cases (9%) of multiple type. Compared with single-style AWE, multi-style AWE had longer operation time (P P P = 0.035), longer operation time (P P P P P P < 0.01).
文摘目的:基于网络药理学的方法探讨中药黄芩–夏枯草–金银花治疗子宫内膜异位症的作用机制。方法:使用中药系统药理学数据库与分析平台(TCMSP)搜索黄芩、夏枯草及金银花的有效活性成分及作用靶点,通过GeneCards、OMIM和DisGeNet数据库筛选子宫内膜异位症疾病的相关靶点。使用Venny 2.1在线工具构建黄芩–夏枯草–金银花作用靶点与子宫内膜异位症疾病靶点韦恩图,获得交集靶点基因。利用Cytoscape 3.7.1软件对药物–成分–交集靶点网络进行可视化构建,同时将获得的交集靶点基因上传至STRING网站进行蛋白质–蛋白质相互作用分析,使用Metascape在线分析平台对交集靶点进行GO富集分析与KEGG通路富集分析。结果:筛选获得黄芩–夏枯草–金银花的有效活性成分共52个,黄芩–夏枯草–金银花与子宫内膜异位症交集靶点有140个,相关重要靶点包括AKT1、TNF、IL6、TP53、IL1B等。GO富集分析结果显示涉及1878个生物过程,76个细胞组分,196个分子功能。KEGG通路富集获得205条相关信号通路,主要涉及的通路有PI3K-Akt信号通路、IL-17信号通路、TNF信号通路等。结论:黄芩–夏枯草–金银花可能通过多靶点、多通路发挥治疗子宫内膜异位症的作用。Objective: To explore the mechanism of action of traditional Chinese medicine Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos in the treatment of endometriosis based on network pharmacology. Methods: The Traditional Chinese Medicine Systems Pharmacology Database and Analysis Platform (TCMSP) was used to search for effective active ingredients and targets of Scutellariae Radix, Spica Prunellae, Lonicerae Japonicae Flos, and related targets for endometriosis diseases were screened through GeneCards, OMIM and DisGeNet databases. Use the Venny 2.1 online tool to construct a Venn diagram of Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos action targets and endometriosis disease targets, and obtain intersection target genes. Cytoscape 3.7.1 software was used to visually construct the drug-component-intersection target network, and the obtained intersection target genes were uploaded to the STRING website for protein-protein interaction analysis. The Metascape online analysis platform was used to perform GO enrichment analysis and KEGG pathway enrichment analysis on the intersection targets. Results: A total of 52 effective active ingredients of Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos were screened, and there were 140 intersection targets between Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos and endometriosis. Related important targets include AKT1, TNF, IL6, TP53, IL1B, etc. GO enrichment analysis results showed that 1878 biological processes, 76 cellular components, and 196 molecular functions were involved. KEGG pathway enrichment obtained 205 related signaling pathways, the main pathways involved include the PI3K-Akt signaling pathway, IL-17 signaling pathway, TNF signaling pathway, etc. Conclusion: Scutellariae Radix-Spica Prunellae-Lonicerae Japonicae Flos may play a role in treating endometriosis through multiple targets and multiple pathways.
