Neurofibromatosis type 2 is a well known disease of the human skin. Its microscopic and ultramicroscopic features are also well defined. Aim： The aim of this work was to study the involvement of catecholaminergic ner...Neurofibromatosis type 2 is a well known disease of the human skin. Its microscopic and ultramicroscopic features are also well defined. Aim： The aim of this work was to study the involvement of catecholaminergic nerve fibers in the human skin neurofibromatosis. Bioptic fragments of the human skin have been harvested from healthy and diseased subjects. On these specimens the following analysis were performed： 1） light microscopic observation after colouring with hematoxyline-Eosine. 2） lmmunochemical staining for Protein Gene Product 9.5.3） Fluorescent staining for catecholaminergic nerve fibers. 4） Quantitative analysis of images by means of the Quantimet analyzer Leica. 5） Statistical analysis of the quantitative morphological data comparing the healthy with diseased subjects. Comparing the light microscopy images in normal and pathological subjects the authors can affirm that the skin neurofibromatosis induces a strong decrease of the nerve fibers cutted in small pieces and destroyed. Also the catecholaminergic nerve fibers are strongly reduced and destroyed. Quantitative analysis of images and statistical analysis of the morphological data confirm that neurofibromatosis induces strong changes of the skin nerve fibers. The authors＇ results confirm that the neurofibromatosis type 2 induces an almost total destruction of the skin nerve fibers.展开更多
The denominations of origin being perceived by the consumers in terms of both intrinsic qualities and of extrinsic qualities, have contributed in no uncertain manner to the increase of the typical products in question...The denominations of origin being perceived by the consumers in terms of both intrinsic qualities and of extrinsic qualities, have contributed in no uncertain manner to the increase of the typical products in question, positively influenced also by the noted emergencies coming from verified alimentary alarms during the beginning of the XXI century. The objective of this paper, is to determine the habits of purchase of typical and traditional products of the people of Messina （Sicily, Italy）. To do this a ＂motivational＂ survey was carried out by traditional methodology of descriptive researches. The adopted methodology of research was quantitative and random. The random research strives to identify the factors that depend on the behaviour of purchase and the appraisal of the existing relationships of cause-effect in a determined population. The interviewed were given a questionnaire composed of 17 questions on their knowledge of the typical products characteristics, the frequency of consumption, the monthly amounts destined to the purchase, the motivations that push them to the purchase of typical products rather than pre-packed. Methodologically, our statistical analysis has been performed using the log-likelihood ratio test in order to investigate the existence of association between the joint distributions of qualitative measured variables.展开更多
Singular authorship of＂The Tale of Genji,＂ which is the greatest accomplishment in classical Japanese literature, is doubtful In this study, we statistically analyze whether the author of Uji Jujo, which comprises t...Singular authorship of＂The Tale of Genji,＂ which is the greatest accomplishment in classical Japanese literature, is doubtful In this study, we statistically analyze whether the author of Uji Jujo, which comprises the last ten chapters of the ＂Tale of Ganji,＂ is same as that of other chapters by using statistical analyses such as principal component analysis and random forests. The result of the analyses of word frequency shows that there is no obvious difference in word usage between the ten chapters and others. Thus, we conclude that it is highly possible that the chapters＇ author is same as that of others.展开更多
Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present s...Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present study, we established an ultra high performance liquid chromatography （UHPLC） coupled with quadmpole/time-of-flight mass spectrometry （Q/TOF-MS） method for the rapid analysis of eight AA analogues in 19 samples originated from the five Aristolochiaceae plants, the roots and rhizomes ofAsarum sieboldii Miq. var. seoulense Nakai, the fruits ofAristolochia contorta Bunge or A. debilis Sieb. et Zucc., the roots ofAristolochia debilis Sieb. et Zucc., the stems ofAristolochia manshuriensis Kom., and the roots of Aristolochiafangchi Y. C. Wu ex L. D. Chou et S. M. Hwang. A total of five AAs and three ALs were identified by co-chromatography of sample extract and comparing the retention time, UV spectra, and characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification or semi-quantification of them. The samples significantly differed in the quality and quantity of AA analogues, which aUowed the possibility of showing their chemical distinctness, and it might be helpful in their standardization and quality control. Furthermore, in order to holistically compare the difference between the five Aristolochiaceae plants, dataset obtained from UHPLC-Q/TOF-MS was processed with principal component analysis （PCA） and orthogonal partial least squared discriminant analysis （OPLS-DA）.展开更多
It is important to study the contributions of climate change and human activities to cropland changes in the fields of both climate change and land use change. Relationships between cropland changes and driving forces...It is important to study the contributions of climate change and human activities to cropland changes in the fields of both climate change and land use change. Relationships between cropland changes and driving forces were qualitatively studied in most of the previous researches. However, the quantitative assessments of the contributions of climate change and human activities to cropland changes are needed to be explored for a better understanding of the dynamics of land use changes. We systematically reviewed the methods of identifying the contributions of climate change and human activities to cropland changes at quantitative aspects, including model analysis, mathematical statistical method, framework analysis, index assessment and difference comparison. Progress of the previous researches on quantitative evaluation of the contributions was introduced. Then we discussed four defects in the assessment of the contributions of climate change and human activities. For example, the methods were lack of comprehensiveness, and the data need to be more accurate and abundant. In addition, the scale was single and the explanations were biased. Moreover, we concluded a clue about quantitative approach to assess the contributions from synthetically aspect to specific driving forces. Finally, the solutions of the future researches on data, scale and explanation were proposed.展开更多
文摘Neurofibromatosis type 2 is a well known disease of the human skin. Its microscopic and ultramicroscopic features are also well defined. Aim： The aim of this work was to study the involvement of catecholaminergic nerve fibers in the human skin neurofibromatosis. Bioptic fragments of the human skin have been harvested from healthy and diseased subjects. On these specimens the following analysis were performed： 1） light microscopic observation after colouring with hematoxyline-Eosine. 2） lmmunochemical staining for Protein Gene Product 9.5.3） Fluorescent staining for catecholaminergic nerve fibers. 4） Quantitative analysis of images by means of the Quantimet analyzer Leica. 5） Statistical analysis of the quantitative morphological data comparing the healthy with diseased subjects. Comparing the light microscopy images in normal and pathological subjects the authors can affirm that the skin neurofibromatosis induces a strong decrease of the nerve fibers cutted in small pieces and destroyed. Also the catecholaminergic nerve fibers are strongly reduced and destroyed. Quantitative analysis of images and statistical analysis of the morphological data confirm that neurofibromatosis induces strong changes of the skin nerve fibers. The authors＇ results confirm that the neurofibromatosis type 2 induces an almost total destruction of the skin nerve fibers.
文摘The denominations of origin being perceived by the consumers in terms of both intrinsic qualities and of extrinsic qualities, have contributed in no uncertain manner to the increase of the typical products in question, positively influenced also by the noted emergencies coming from verified alimentary alarms during the beginning of the XXI century. The objective of this paper, is to determine the habits of purchase of typical and traditional products of the people of Messina （Sicily, Italy）. To do this a ＂motivational＂ survey was carried out by traditional methodology of descriptive researches. The adopted methodology of research was quantitative and random. The random research strives to identify the factors that depend on the behaviour of purchase and the appraisal of the existing relationships of cause-effect in a determined population. The interviewed were given a questionnaire composed of 17 questions on their knowledge of the typical products characteristics, the frequency of consumption, the monthly amounts destined to the purchase, the motivations that push them to the purchase of typical products rather than pre-packed. Methodologically, our statistical analysis has been performed using the log-likelihood ratio test in order to investigate the existence of association between the joint distributions of qualitative measured variables.
文摘Singular authorship of＂The Tale of Genji,＂ which is the greatest accomplishment in classical Japanese literature, is doubtful In this study, we statistically analyze whether the author of Uji Jujo, which comprises the last ten chapters of the ＂Tale of Ganji,＂ is same as that of other chapters by using statistical analyses such as principal component analysis and random forests. The result of the analyses of word frequency shows that there is no obvious difference in word usage between the ten chapters and others. Thus, we conclude that it is highly possible that the chapters＇ author is same as that of others.
基金The National Science and Technology Support Program during the Twelfth Five-Year Plan of China(Grant No.2011BAI03B05)the Distinguished Professor Foundation of Liaoning Province of China of 2011Innovative Drug Incubation Base Plan Project from Liaoning Province of China of 2013(Grant No.2013226027)
文摘Aristolochic acids （AAs） and aristololactams （ALs） are commonly found in some Aristolochiaceae plants, and they have been reported to be AA nephropathy （AAN）, nephrotoxicity and carcinogenicity. In the present study, we established an ultra high performance liquid chromatography （UHPLC） coupled with quadmpole/time-of-flight mass spectrometry （Q/TOF-MS） method for the rapid analysis of eight AA analogues in 19 samples originated from the five Aristolochiaceae plants, the roots and rhizomes ofAsarum sieboldii Miq. var. seoulense Nakai, the fruits ofAristolochia contorta Bunge or A. debilis Sieb. et Zucc., the roots ofAristolochia debilis Sieb. et Zucc., the stems ofAristolochia manshuriensis Kom., and the roots of Aristolochiafangchi Y. C. Wu ex L. D. Chou et S. M. Hwang. A total of five AAs and three ALs were identified by co-chromatography of sample extract and comparing the retention time, UV spectra, and characteristic molecular ions and fragment ions with those of authentic standards, or tentatively identified by MS/MS determination along with Mass Fragment software. Moreover, the method was validated for the simultaneous quantification or semi-quantification of them. The samples significantly differed in the quality and quantity of AA analogues, which aUowed the possibility of showing their chemical distinctness, and it might be helpful in their standardization and quality control. Furthermore, in order to holistically compare the difference between the five Aristolochiaceae plants, dataset obtained from UHPLC-Q/TOF-MS was processed with principal component analysis （PCA） and orthogonal partial least squared discriminant analysis （OPLS-DA）.
基金National Natural Science Foundation of China,No.41401113,No.41371002,No.41471091The Science and Technology Strategic Pilot of the Chinese Academy of Sciences,No.XDA05090310The Key Project of Physical Geography of Hebei Province
文摘It is important to study the contributions of climate change and human activities to cropland changes in the fields of both climate change and land use change. Relationships between cropland changes and driving forces were qualitatively studied in most of the previous researches. However, the quantitative assessments of the contributions of climate change and human activities to cropland changes are needed to be explored for a better understanding of the dynamics of land use changes. We systematically reviewed the methods of identifying the contributions of climate change and human activities to cropland changes at quantitative aspects, including model analysis, mathematical statistical method, framework analysis, index assessment and difference comparison. Progress of the previous researches on quantitative evaluation of the contributions was introduced. Then we discussed four defects in the assessment of the contributions of climate change and human activities. For example, the methods were lack of comprehensiveness, and the data need to be more accurate and abundant. In addition, the scale was single and the explanations were biased. Moreover, we concluded a clue about quantitative approach to assess the contributions from synthetically aspect to specific driving forces. Finally, the solutions of the future researches on data, scale and explanation were proposed.
