A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materi...A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.展开更多
A set of numerical experiments is designed and carried out to understand a heat sink in the Southern Ocean in the recent hiatus decade. By using an oceanic general circulation model, the authors focus on the contribut...A set of numerical experiments is designed and carried out to understand a heat sink in the Southern Ocean in the recent hiatus decade. By using an oceanic general circulation model, the authors focus on the contributions from two types of forcing: wind stress and thermohaline forcing. The simulated results show that the heat sink in the upper Southern Ocean comes mainly from thermohaline forcing; while in the deeper layers, wind stress forcing also plays an important role. These different contributions may be due to different physical processes for the heat budget. The combination of these two types of forcing shows a significant heat sink in the Southern Ocean in the recent hiatus decade, and this is consistent with the observations and conclusions of a similar recently published study.展开更多
文摘A non-equilibrium chromatographic rate model was employed to simulate the affinity chromatography of urokinase. The chromatography process was developed to a yield of high purity product of urokinase from crude materials. The affinity gel used in the process was prepared by an epichlorohydrin-activation method using epichlorohydrin activated Sepharose 4B as a matrix and p-aminobenzamidine as a ligand. The chromatographic process were numerically simulated and analyzed with the aid of VERSE-LC computer simulator. Considering the basic principles, rate model with the back mixing in column inlet was utilized in simulating and studying the effect of the column inlet pattern on other parameters. Comparison of the simulation results with the experimental data showed that the rate model can be used to describe the affinity chromatography of urokinase in a fixed bed column with satisfactory accuracy.
基金supported by the National Natural Science Foundation of China (Grant No. 41175058)the Strategic Priority Research Program of the Chinese Academy of Sciences (Grant No. XDA11010404)
文摘A set of numerical experiments is designed and carried out to understand a heat sink in the Southern Ocean in the recent hiatus decade. By using an oceanic general circulation model, the authors focus on the contributions from two types of forcing: wind stress and thermohaline forcing. The simulated results show that the heat sink in the upper Southern Ocean comes mainly from thermohaline forcing; while in the deeper layers, wind stress forcing also plays an important role. These different contributions may be due to different physical processes for the heat budget. The combination of these two types of forcing shows a significant heat sink in the Southern Ocean in the recent hiatus decade, and this is consistent with the observations and conclusions of a similar recently published study.
