5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isol...5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4＇-forrnamidophenyl-5-methoxybenzoate （1）, along with seven known polyketides, 4-hydroxybenzoic acid （2）, LL-P880ct （3）, 1＇-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one （4）, pestalotiollide B （5）, pestalotiopyrone G （6）, endocrocin （7） and 2＇-hydroxy- 6＇-hydroxymethyl-4＇-methylphenyl-2,6-dihydroxy-3-（2-isopentenyl）benzoate （8）. HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.展开更多
基金National Natural Science Foundation(Grant No.31660251)Fundamental Research Funds for the Central Universities(Grant No.0903005203401)the Start-up Fund for the“Hundred Young-Talent Scheme”Professorship(Grant No.0236011104424)
文摘5-Azacytidine, a DNA methyltransferase inhibitor, led to significant changes in the secondary metabolism of the plant endophytic fungus, Pestalotiopsis microspora. Analysis of the culture broth extract led to the isolation of a new compound, 4＇-forrnamidophenyl-5-methoxybenzoate （1）, along with seven known polyketides, 4-hydroxybenzoic acid （2）, LL-P880ct （3）, 1＇-hydroxy-4-methoxy-6-pentyl-2H-pyran-2-one （4）, pestalotiollide B （5）, pestalotiopyrone G （6）, endocrocin （7） and 2＇-hydroxy- 6＇-hydroxymethyl-4＇-methylphenyl-2,6-dihydroxy-3-（2-isopentenyl）benzoate （8）. HPLC profiles revealed that all compounds except for 4 belonged to the newly induced secondary metabolites. In addition, all compounds were proved to be devoid of significant antifungal activity in the bioassays.
