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党参、黄芪、白术提取物配伍应用对小肠上皮细胞增殖的影响 被引量:26
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作者 张子理 陈蔚文 《广州中医药大学学报》 CAS 2002年第2期137-140,共4页
[目的]观察党参、黄芪、白术有效组分配伍对小肠隐窝细胞株(IEC-6)细胞增殖的影响。[方法]IEC-6以每孔50μL-1的密度接种200μL于96孔培养板,每组3孔。24h后加入不同剂量的各种有效组分及其配伍溶液10μL,空白组加等量Dulbecco’s磷酸... [目的]观察党参、黄芪、白术有效组分配伍对小肠隐窝细胞株(IEC-6)细胞增殖的影响。[方法]IEC-6以每孔50μL-1的密度接种200μL于96孔培养板,每组3孔。24h后加入不同剂量的各种有效组分及其配伍溶液10μL,空白组加等量Dulbecco’s磷酸缓冲液。加药后24 h用噻唑蓝(MTT)比色法观察细胞增殖情况。[结果]来源于不同药物或同一药物的两个有效组分配伍应用,对IEC-6细胞的增殖作用表现各异,有的协同促进,有的协同抑制,有的作用相互抵消,有的作用方向改变,有的作用无明显改变。[结论]党参、黄芪、白术不同提取部位配伍应用对IEC-6细胞增殖具有调控作用。 展开更多
关键词 中药 药理 党参 黄芪 白术 提取物 配伍 中药 药理 小肠上皮细胞增殖
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Effect of Dachengqi Tang(大承气汤)Granule on Proliferation ofIntestinal Epithelial Cells in Rats with ExperimentalIntestinal Obstruction
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作者 康毅 林秀珍 《Chinese Journal of Integrated Traditional and Western Medicine》 2003年第1期57-59,共3页
Objective: To study the effects of Dachengqi Tang (DCQT) granule on the proliferation of the intestinal epithelial cells in rats with experimental intestinal obstruction. Methods: Experimental intestinal obstruction m... Objective: To study the effects of Dachengqi Tang (DCQT) granule on the proliferation of the intestinal epithelial cells in rats with experimental intestinal obstruction. Methods: Experimental intestinal obstruction models were established in rats and autoradiography with 3H-TdR was used to determine 3H-TdR labeling counts of intestinal epithelial cells in rats. Results: DCQT granule had no effects on 3H-TdR labeling counts of intestinal epithelial cells in normal rats. DCQT granule obviously increases the rate of renovation in intestinal epithelial cells of the intestinal obstruction rats. Conclusion: DCQT granule could reinforce the intestinal mucosa's defensive function by means of increasing the proliferation of intestinal epithelial cells. 展开更多
关键词 大鼠 实验性肠梗阻 大承气汤 小肠上皮细胞增殖 防卫功能
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