AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactiv...AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactivity assay after precipitation with 200 mL/L trichloroacetic add (TCA-RA method) were used to dete-rmine the tissue distribution,and the urinary and fecal excretions of 125I-C-1027 in mice and its biliary excretion in rats. RESULTS:Tissue concentrations reached the peak at the fifth minute after administration of 125I-C-1027 to mice. The highest concentration was in kidney, and the lowest in brain at all test-time points. The organs of the concentrations of 125I-C-1027 from high to low were kidney, lung, liver, stomach, spleen, uterus, ovary, intestine, muscle, heart, testis, fat, and brain in mice. The accumulative excretion amounts of 0-24 h, and 0-96 h after administration of 125I-C-1027 were 68.36 and 71.64% in urine, and 2.60 and 3.21% in feces of mice, respectively, and the accumulative excretion amount of 0-24 h was 3.57% in bile in rats. CONCLUSION: Our results reflect the characteristics of the tissue distribution, urinary and fecal excretions of 125I-C-1027 in mice and the biliary excretion of 125I-C-1027 and its metabolites in rats, and indicate that 125I-C-1027 and its metabolites are mainly distributed in kidney, and excreted in urine.展开更多
The objectives of the study were to investigate the effects of different levels of urea treatments on chemical composition, nutritional value and rumen degradability of sorghum stover. Two groups of animals were used ...The objectives of the study were to investigate the effects of different levels of urea treatments on chemical composition, nutritional value and rumen degradability of sorghum stover. Two groups of animals were used in this experiment. The first group, which served as control, was offered untreated sorghum stover. The other, the experiment group recived sorghum stover to which was added 2%, or 4% urea. Crude protein （CP） content of the treated sorghum was enhanced （P 〈 0.05） over the untreated one, and ranked as 7.30% and 6.05% CP for 4% and 2% respectively. Nutrient detergent fiber （NDF） content was decreased （P 〈 0.05） from 92.5% （control） to 89.8% and 87.8% for 2% and 4% urea respectively.Ether extract （EE） content was decreased （P 〈 0.05） from 1.77% （control） to 1.63% and 0.82% for 2% and 4% respectively. The rumen degradability was increased （P 〈 0.05） from 59.2% （control） to 64.2%, and 67.2% for 2%, and 4% sorghum and in hours 0, 4, 8, 16, 24, 48, 72 and 96.展开更多
基金Supported by the National High Technology Research and Development Program of China (863 Program), No. 2003AA2Z347D
文摘AIM: To investigate the tissue distribution, urinary and fecal excretions of 125I-lidamycin (125I-C-1027) in mice and its biliary excretion in rats. METHODS:The total radioactivity assay (RA method) and the radioactivity assay after precipitation with 200 mL/L trichloroacetic add (TCA-RA method) were used to dete-rmine the tissue distribution,and the urinary and fecal excretions of 125I-C-1027 in mice and its biliary excretion in rats. RESULTS:Tissue concentrations reached the peak at the fifth minute after administration of 125I-C-1027 to mice. The highest concentration was in kidney, and the lowest in brain at all test-time points. The organs of the concentrations of 125I-C-1027 from high to low were kidney, lung, liver, stomach, spleen, uterus, ovary, intestine, muscle, heart, testis, fat, and brain in mice. The accumulative excretion amounts of 0-24 h, and 0-96 h after administration of 125I-C-1027 were 68.36 and 71.64% in urine, and 2.60 and 3.21% in feces of mice, respectively, and the accumulative excretion amount of 0-24 h was 3.57% in bile in rats. CONCLUSION: Our results reflect the characteristics of the tissue distribution, urinary and fecal excretions of 125I-C-1027 in mice and the biliary excretion of 125I-C-1027 and its metabolites in rats, and indicate that 125I-C-1027 and its metabolites are mainly distributed in kidney, and excreted in urine.
文摘The objectives of the study were to investigate the effects of different levels of urea treatments on chemical composition, nutritional value and rumen degradability of sorghum stover. Two groups of animals were used in this experiment. The first group, which served as control, was offered untreated sorghum stover. The other, the experiment group recived sorghum stover to which was added 2%, or 4% urea. Crude protein （CP） content of the treated sorghum was enhanced （P 〈 0.05） over the untreated one, and ranked as 7.30% and 6.05% CP for 4% and 2% respectively. Nutrient detergent fiber （NDF） content was decreased （P 〈 0.05） from 92.5% （control） to 89.8% and 87.8% for 2% and 4% urea respectively.Ether extract （EE） content was decreased （P 〈 0.05） from 1.77% （control） to 1.63% and 0.82% for 2% and 4% respectively. The rumen degradability was increased （P 〈 0.05） from 59.2% （control） to 64.2%, and 67.2% for 2%, and 4% sorghum and in hours 0, 4, 8, 16, 24, 48, 72 and 96.
