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质粒在大肠杆菌和吸水链霉菌井冈5008之间的属间接合转移 被引量:1
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作者 陈双雅 《漳州师范学院学报(自然科学版)》 1999年第1期54-58,共5页
利用一个链霉菌──大肠杆菌双功能柯斯载体pHZ132建立了吸水链霉菌井冈变种5008(以下简称井冈5008)的属间接合转移系统.将携带pHZ132的大肠杆菌细胞LE392和携带RP4衍生质粒pRK2073的大肠杆菌细胞ED8768与萌发后的井冈5008孢子混合... 利用一个链霉菌──大肠杆菌双功能柯斯载体pHZ132建立了吸水链霉菌井冈变种5008(以下简称井冈5008)的属间接合转移系统.将携带pHZ132的大肠杆菌细胞LE392和携带RP4衍生质粒pRK2073的大肠杆菌细胞ED8768与萌发后的井冈5008孢子混合起来进行三亲本杂交,在接合性质粒RP4转移功能的诱导下,pHZ132即可从大肠杆菌细胞LE392转移到井冈5008孢子中去.所用培养基是利用2CM:LA=4:1(体积比)的混合培养基,井冈5008最佳孢子萌发时间为3.5~4小时. 展开更多
关键词 吸水链霉菌井冈5008 大肠杆菌 属间接合转移
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一个可在大肠杆菌和链霉菌之间进行属间接合转移的柯斯质粒 被引量:4
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作者 鲍锴 胡志浩 +2 位作者 周秀芬 周启 邓子新 《科学通报》 EI CAS CSCD 北大核心 1995年第15期1440-1440,共1页
利用柯斯质粒载体在大肠杆菌中克隆和分析链霉菌基因簇的方法和技术已有很大发展,已构建了不少链霉菌-大肠杆菌双功能柯斯载体.利用这种载体固然可以把链霉菌的基因文库构建在大肠杆菌中并可方便地进行克隆片段的结构分析,但要通过转化... 利用柯斯质粒载体在大肠杆菌中克隆和分析链霉菌基因簇的方法和技术已有很大发展,已构建了不少链霉菌-大肠杆菌双功能柯斯载体.利用这种载体固然可以把链霉菌的基因文库构建在大肠杆菌中并可方便地进行克隆片段的结构分析,但要通过转化把克隆的大片段DNA 转回到链霉菌中来进行功能分析常常遇到不少障碍,最近发展起来的质粒在大肠杆菌和链霉菌之间进行属间接合转移的方法,则解决了外源DNA导入许多链霉菌菌种的疑难问题。 展开更多
关键词 大肠杆菌 链霉菌 属间接合转移 柯斯质粒
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抗生素Bagremycin生产菌Streptomyces sp. Tü4128接合转移系统的建立 被引量:2
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作者 朱云霞 廖思懿 +1 位作者 叶江 张惠展 《华东理工大学学报(自然科学版)》 CAS CSCD 北大核心 2012年第2期160-164,共5页
基于噬菌体φC31 att/int系统,采用属间接合将来自大肠杆菌的DNA有效地导入菌株Tü4128的孢子中。通过条件优化,当融合温度为37℃时,在含80mmol/L MgCl2的ISP4培养基上使每个受体细胞的接合频率显著提高到7.3×10-3。Southern b... 基于噬菌体φC31 att/int系统,采用属间接合将来自大肠杆菌的DNA有效地导入菌株Tü4128的孢子中。通过条件优化,当融合温度为37℃时,在含80mmol/L MgCl2的ISP4培养基上使每个受体细胞的接合频率显著提高到7.3×10-3。Southern blot分析显示在受体菌染色体上存在单一的染色体附着位点(attB位点)。整合位点的DNA序列测序显示为保守。采用优化的方法建立了大肠杆菌和菌株Tü4128间高效的属间接合系统。 展开更多
关键词 attB位点 噬菌体φC31 bagremycin 属间接合 STREPTOMYCES sp. Tü4128
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Streptomyces lincolnensis新遗传操作方法的建立以及lmbQ基因功能研究 被引量:8
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作者 王增亮 赵群飞 +2 位作者 高淑红 陈长华 刘文 《生物技术通报》 CAS CSCD 北大核心 2010年第11期186-190,198,共6页
以载体pSET152和pKC1139为出发质粒,通过供体菌大肠杆菌ET12567和S17-1转入林可链霉菌(Streptomyces lincolnensis),建立和优化了接合转移体系。林可霉素生物合成基因簇中,lmbQ基因可能编码一个调控蛋白,构建了S.lin-colnensislmbQ基因... 以载体pSET152和pKC1139为出发质粒,通过供体菌大肠杆菌ET12567和S17-1转入林可链霉菌(Streptomyces lincolnensis),建立和优化了接合转移体系。林可霉素生物合成基因簇中,lmbQ基因可能编码一个调控蛋白,构建了S.lin-colnensislmbQ基因中断载体,通过接合转移导入S.lincolnensis野生型菌株,筛选得到基因双交换突变株,通过PCR以及测序验证基因型正确,该突变株即为lmbQ同框敲除突变株。摇瓶发酵结果表明,lmbQ基因是一个正调控基因。 展开更多
关键词 林可链霉菌 林可霉素 属间接合转移 lmbQ基因 同框敲除
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海洋链霉菌分离株M095遗传转化体系的建立 被引量:1
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作者 侯艳华 王淑军 +1 位作者 李富超 秦松 《海洋科学》 CAS CSCD 北大核心 2006年第11期12-16,共5页
研究了海洋链霉菌分离株M095的基因转移系统。利用属间接合转移将具有oriT的大肠杆菌-链霉菌穿梭质粒pIJ8600转入EscherichiacoliET12567(pUZ8002)中,获得供体菌。将供体菌与预萌发的菌株M095的孢子进行接合转移,将质粒pIJ8600转入菌株M... 研究了海洋链霉菌分离株M095的基因转移系统。利用属间接合转移将具有oriT的大肠杆菌-链霉菌穿梭质粒pIJ8600转入EscherichiacoliET12567(pUZ8002)中,获得供体菌。将供体菌与预萌发的菌株M095的孢子进行接合转移,将质粒pIJ8600转入菌株M095中,其转化率为1.99×10-4个接合转化子/受体。Southern杂交证明质粒pIJ8600已经整合到菌株M095的染色体上。同时,将来自Spirulinamaxima(Cyanophyta)的别藻蓝蛋白基因(apc)克隆在质粒pIJ8600的XbaI和BglII位点,产生质粒pAPIJ。用接合转移法将质粒pAPIJ转入菌株M095。通过SDS-PAGE分析,得到2个大小为22ku和17ku的蛋白,分别相对应于别藻蓝蛋白的α和β亚基。这些结果进一步证明菌株M095的遗传转化体系已经成功建立起来,这将为其他海洋放线菌遗传转化工作的研究奠定基础。 展开更多
关键词 转化 全霉素 属间接合转移 海洋链霉菌
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透明颤菌血红蛋白基因在弗氏链霉菌中的表达及其对菌体生长和泰乐星合成的影响 被引量:9
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作者 陈文青 喻子牛 郑应华 《中国抗生素杂志》 CAS CSCD 北大核心 2004年第9期516-520,共5页
质粒 p IJ4 0 2 6和 p RK4 0 4 - vhb分别含有红霉素抗性基因启动子 (Perm E)和透明颤菌血红蛋白基因 (vhb) ,先用 PCR分别扩增二者 ,再用 SOE- PCR(Splicing by overlap extension PCR)将不含自身启动子的vhb基因置于 Perm E之下 ;将 S... 质粒 p IJ4 0 2 6和 p RK4 0 4 - vhb分别含有红霉素抗性基因启动子 (Perm E)和透明颤菌血红蛋白基因 (vhb) ,先用 PCR分别扩增二者 ,再用 SOE- PCR(Splicing by overlap extension PCR)将不含自身启动子的vhb基因置于 Perm E之下 ;将 SOE- PCR产物克隆到链霉菌整合性载体 p SET15 2上 ,构建成 vhb基因表达载体 p WQ2 0 0 4。通过大肠埃希氏菌 -链霉菌属间接合转移的方式将 p WQ2 0 0 4导入弗氏链霉菌 (Streptomycesfradiae)中 ,利用 p WQ2 0 0 4上 Φ C31整合性位点使 vhb基因整合到弗氏链霉菌染色体上。PCR验证表明 ,vhb基因已整合到染色体上 ,CO结合差光谱分析则表明 vhb基因在弗氏链霉菌中成功表达。摇瓶与上罐发酵显示 展开更多
关键词 uhb基因 SOE-PCR 属间接合转移 CO结合差光谱分析 泰乐星
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生二素链霉菌orf1~*c基因的功能研究及菌株改造 被引量:1
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作者 刘德玉 赵群飞 +3 位作者 高淑红 叶蕊芳 陈长华 刘文 《江苏农业学报》 CSCD 北大核心 2013年第1期39-45,共7页
研究螺旋霉素生物合成基因中orf1*c的功能,优化菌株,减少出发菌株发酵液m/z为875的杂质组分。试验选取pKC1139和pSET152作为质粒载体,构建重组质粒pKC1139-orf1*c和pSET-orf1*c。然后分别对螺旋霉素工业生产菌株Streptomyce ambofaciens... 研究螺旋霉素生物合成基因中orf1*c的功能,优化菌株,减少出发菌株发酵液m/z为875的杂质组分。试验选取pKC1139和pSET152作为质粒载体,构建重组质粒pKC1139-orf1*c和pSET-orf1*c。然后分别对螺旋霉素工业生产菌株Streptomyce ambofaciens的orf1*c基因进行敲除和倍增,得到相应的突变株L-D-1*c和L-P1*c-7,将突变株进行发酵、生物效价测定和LC-MS检测。发现突变株L-D-1*c完全丧失了生产螺旋霉素组分的能力并产生了相应的中间产物,突变株L-P1*c-7具有有效减少m/z为875杂质组分的生产能力。通过序列比对和中间产物分析,表明基因orf1*c负责编码螺旋霉素合成中的N-二甲基转移酶,得到了初步的优化菌株L-P1*c-7。 展开更多
关键词 生二素链霉菌 螺旋霉素 属间接合转移 基因倍增
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Effect of immersion Ni plating on interface microstructure and mechanical properties of Al/Cu bimetal 被引量:5
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作者 赵佳蕾 接金川 +3 位作者 陈飞 陈航 李廷举 曹志强 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2014年第6期1659-1665,共7页
A nickel-based coating was deposited on the pure Al substrate by immersion plating,and the Al/Cu bimetals were prepared by diffusion bonding in the temperature range of 450-550 ℃.The interce microstructure and fractu... A nickel-based coating was deposited on the pure Al substrate by immersion plating,and the Al/Cu bimetals were prepared by diffusion bonding in the temperature range of 450-550 ℃.The interce microstructure and fracture surface of Al/Cu joints were studied by scanning electron microscopy(SEM) and X-ray diffraction(XRD).The mechanical properties of the Al/Cu bimetals were measured by tensile shear and microhardness tests.The results show that the Ni interiayer can effectively eliminate the formation of Al-Cu intermetallic compounds.The Al/Ni interface consists of the Al3Ni and Al3Ni2 phases,while it is Ni-Cu solid solution at the Ni/Cu interce.The tensile shear strength of the joints is improved by the addition of Ni interiayer.The joint with Ni interiayer annealed at 500 ℃ exhibits a maximum value of tensile shear strength of 34.7 MPa. 展开更多
关键词 Al/Cu bimetal immersion Ni plating INTERFACE diffusion bonding INTERMETALLICS
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Influence of electron-beam superposition welding on intermetallic layer of Cu/Ti joint 被引量:3
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作者 陈国庆 张秉刚 +1 位作者 刘伟 冯吉才 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第10期2416-2420,共5页
QCr0.8 was electron-beam welded to TC4 and the effect of the intermetallic layer (IMC-layer) on the mechanical properties of the joint was investigated. The IMC-layers are joint weaknesses at the Cu fusion line in c... QCr0.8 was electron-beam welded to TC4 and the effect of the intermetallic layer (IMC-layer) on the mechanical properties of the joint was investigated. The IMC-layers are joint weaknesses at the Cu fusion line in centered welding and at the Ti fusion line when the beam is deviated towards Cu. A new method referred to as electron-beam superposition welding was presented, and the optimal welding sequence was considered. The IMC-layer produced by centered welding was fragmented and remelted during Cu-side non-centered welding, giving a finely structured compound layer and improved mechanical properties of the joint. The tensile strength of joint is 276.0 MPa, 76.7% that of the base metal. 展开更多
关键词 Cu alloy Ti alloy electron beam superposition welding IMC-layer
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Microstructures and properties of welded joint of aluminum alloy to galvanized steel by Nd:YAG laser + MIG arc hybrid brazing-fusion welding 被引量:5
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作者 秦国梁 苏玉虎 王术军 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2014年第4期989-995,共7页
According to the differences in melting point between aluminum alloy and steel, 6013-T4 aluminum alloy was joined to galvanized steel by large spot Nd:YAG laser + MIG arc hybrid brazing-fusion welding with ER4043(A... According to the differences in melting point between aluminum alloy and steel, 6013-T4 aluminum alloy was joined to galvanized steel by large spot Nd:YAG laser + MIG arc hybrid brazing-fusion welding with ER4043(AlSi5) filler wire. The microstructures and mechanical properties of the brazed-fusion welded joint were investigated. The joint is divided into two parts of fusion weld and brazed seam. There is a zinc-rich zone at fusion weld toe, which consists of α(Al)-Zn solid solution and Al-Zn eutectic. The brazed seam is the Fe-Al intermetallic compounds (IMCs) layer of 2-4μm in thickness, and the IMCs include FeAl2, Fe2Al5 and Fe4Al13. FeAl2 and Fe2Al5 are located in the compact reaction layer near the steel side, and Fe4Al13 with tongue shape or sawtooth shape grows towards the fusion weld. The tensile strength of the joint firstly increases and then decreases as the welding current and laser power increase, the highest tensile strength can be up to 247.3 MPa, and the fracture usually occurs at fusion zone of the fusion weld. The hardness is the highest at the brazed seam because of hard Fe-Al IMCs, and gradually decreases along the fusion weld and galvanized steel, respectively. 展开更多
关键词 brazing-fusion weldingi welding of dissimilar metals hybrid welding mechanical properties intermetallic compounds
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Diffusion bonding of Al 7075 and Mg AZ31 alloys:Process parameters, microstructural analysis and mechanical properties 被引量:7
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作者 Seyyed Salman SEYYED AFGHAHI Mojtaba JAFARIAN +1 位作者 Moslem PAIDAR Morteza JAFARIAN 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第7期1843-1851,共9页
Al 7075 and Mg AZ31 alloys were joined by diffusion bonding method. Joining process was performed in pressure range of 10-35 MPa at temperatures of 430-450 ℃ for 60 min under a vacuum of 13.3 MPa. The microstructure ... Al 7075 and Mg AZ31 alloys were joined by diffusion bonding method. Joining process was performed in pressure range of 10-35 MPa at temperatures of 430-450 ℃ for 60 min under a vacuum of 13.3 MPa. The microstructure evaluation, phase analysis and distribution of elements at the interface were done using scanning electron microscopy (SEM), energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). The pressure of 25 MPa was determined as the optimum pressure in which the minimum amount of plastic deformation takes place at the joint. Different reaction layers containing intermetallic compounds, such as Al12Mg17, Al3Mg2 andα(Al) solid solution, were observed, in interfacial transition zone (ITZ). Thickness of layers was increased with increasing the operating temperature. According to the results, diffusion of aluminum atoms into magnesium alloy was more and the interface movement towards the Al alloy was observed. The maximum bond strength of 38 MPa was achieved at the temperature of 440 ℃ and pressure of 25 MPa. Fractography studies indicated that the brittle fracture originated from Al3Mg2 phase. 展开更多
关键词 Al 7075 alloy Mg AZ31 alloy diffusion bonding intermetallic compounds interfacial transition zone MICROSTRUCTURE mechanical properties
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Effect of postweld heat treatment on interface microstructure and metallurgical properties of explosively welded bronze–carbon steel 被引量:5
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作者 KHANZADEH GHARAHSHIRAN Mohammad Reza KHOSHAKHLAGH Ali +2 位作者 KHALAJ Gholamreza BAKHTIARI Hamid BANIHASHEMI Ali Reza 《Journal of Central South University》 SCIE EI CAS CSCD 2018年第8期1849-1861,共13页
The effects of postweld heat treatment on the microstructure and metallurgical properties of a bronze–carbon steel(st37)explosively bonded interface were studied.Explosive welding was done under 1.5-and 2-mm standoff... The effects of postweld heat treatment on the microstructure and metallurgical properties of a bronze–carbon steel(st37)explosively bonded interface were studied.Explosive welding was done under 1.5-and 2-mm standoff distances and different conditions of explosive charge.Samples were postweld heat treated for 4 and 16 h in the furnace at 250°C and 500°C and then air cooled.Laboratory studies using optical microscopy,scanning electron microscopy,and microhardness testing were used to evaluate the welded samples.Microstructural examinations showed that by increasing the standoff distance and the explosive charge,the interface of bronze to steel became wavier.The microhardness test result showed that the hardness of the samples was higher near the joint interface compared with other areas because of the intensive plastic deformation,which was caused by the explosion force.The results show that increasing the heat treatment temperature and time caused the intermetallic compounds’layer thickness to increase,and,because of the higher diffusion of copper and tin,the iron amount in the intermetallic compounds decreased.Also,because of the increase in heat treatment temperature and time,internal stresses were released,and the interface hardness decreased. 展开更多
关键词 heat treatment explosive welding intermetallic compound standoff distance diffusion layer
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Interfacial features of TiAl alloy/316L stainless steel joint brazed with Zr−Cu−Ni−Al amorphous filler metal 被引量:9
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作者 Hong-gang DONG Run-ze ZHANG +2 位作者 Yue-qing XIA Xiao-hu HAO Peng LI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第6期1680-1688,共9页
TiAl alloy and 316L stainless steel were vacuum-brazed with Zr−50.0Cu−7.1Ni−7.1Al(at.%)amorphous filler metal.The influence of brazing time and temperature on the interfacial microstructure and shear strength of the r... TiAl alloy and 316L stainless steel were vacuum-brazed with Zr−50.0Cu−7.1Ni−7.1Al(at.%)amorphous filler metal.The influence of brazing time and temperature on the interfacial microstructure and shear strength of the resultant joints was investigated.The brazed seam consisted of three layers,including two diffusion layers and one residual filler metal layer.The typical microstructure of brazed TiAl alloy/316L stainless steel joint was TiAl alloy substrate/α2-(Ti3Al)/AlCuTi/residual filler metal/Cu9Zr11+Fe23Zr6/Laves-Fe2Zr/α-(Fe,Cr)/316L stainless steel substrate.Discontinuous brittle Fe2Zr layer formed near the interface between the residual filler metal layer andα-(Fe,Cr)layer.The maximum shear strength of brazed joints reached 129 MPa when brazed at 1020℃ for 10 min.The diffusion activation energies ofα2-(Ti3Al)andα-(Fe,Cr)phases were−195.769 and−112.420 kJ/mol,respectively,the diffusion constants for these two phases were 3.639×10^(−6) and 7.502×10^(−10)μm^(2)/s,respectively.Cracks initiated at Fe2Zr layer and propagated into the residual filler metal layer during the shear test.The Laves-Fe2Zr phase existing on the fracture surface suggested the brittle fracture mode of the brazed joints. 展开更多
关键词 vacuum brazing dissimilar joining Zr-based amorphous filler metal intermetallic compound growth kinetics
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Corrosion evaluation of friction stir welded lap joints of AA6061-T6 aluminum alloy 被引量:5
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作者 Farhad GHARAVI Khamirul A.MATORI +2 位作者 Robiah YUNUS Norinsan K.OTHMAN Firouz FADAEIFARD 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2016年第3期684-696,共13页
Corrosion behavior of friction stir lap welded AA6061-T6 aluminum alloy was investigated by immersion tests in sodium chloride + hydrogen peroxide solution. Electrochemical measurement by cyclic potentiodynamic polari... Corrosion behavior of friction stir lap welded AA6061-T6 aluminum alloy was investigated by immersion tests in sodium chloride + hydrogen peroxide solution. Electrochemical measurement by cyclic potentiodynamic polarization, scanning electron microscopy, and energy dispersive spectroscopy were employed to characterize corrosion morphology and to realize corrosion mechanism of weld regions as opposed to the parent alloy. The microstructure and shear strength of welded joint were fully investigated. The results indicate that, compared with the parent alloy, the weld regions are susceptible to intergranular and pitting attacks in the test solution during immersion time. The obtained results of lap shear testing disclose that tensile shear strength of the welds is 128 MPa which is more than 60% of the strength of parent alloy in lap shear testing. Electrochemical results show that the protection potentials of the WNZ and HAZ regions are more negative than the pitting potential. This means that the WNZ and HAZ regions do not show more tendencies to pitting corrosion. Corrosion resistance of parent alloy is higher than that for the weldments, and the lowest corrosion resistance is related to the heat affected zone. The pitting attacks originate from the edge of intermetallic particles as the cathode compared with the Al matrix due to their high self-corrosion potential. It is supposed that by increasing intermetallic particle distributed throughout the matrix of weld regions, the galvanic corrosion couples are increased, and hence decrease the corrosion resistance of weld regions. 展开更多
关键词 friction stir welding lap joints AA6061 alloy pitting corrosion welding process intermetallic particles
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Effect of Mg and Si on intermetallic formation and fracture behavior of pure aluminum-galvanized carbon-steel joints made by weld-brazing 被引量:1
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作者 A.ALIKHANI R.BEYGI +2 位作者 M.ZAREZADEH MEHRIZI F.NEMATZADEH I.GALVÃO 《Journal of Central South University》 SCIE EI CAS CSCD 2021年第11期3626-3638,共13页
Commercial pure aluminum and galvanized carbon steel were lap-welded using the weld-brazing(WB)technique.Three types of aluminum filler materials(4043,4047,and 5356) were used for WB.The joint strength and intermetall... Commercial pure aluminum and galvanized carbon steel were lap-welded using the weld-brazing(WB)technique.Three types of aluminum filler materials(4043,4047,and 5356) were used for WB.The joint strength and intermetallic compounds at the interface of three series of samples were analyzed and compared.Depending on the Si content,a variety of ternary Al-Fe-Si intermetallic compounds(IMCs) such as Fe_(4)(Al,Si)_(13),Fe_(2) Al_(8) Si(τ_(5)),and Fe_(2) Al_(9) Si_(2)(τ_(6)) were formed at the interface.Mg element in 5356 filler material cannot contribute to the formation of Al-Fe intermetallic phases due to the positive mixing enthalpy of Mg-Fe.The presence of Mg enhances the hot cracking phenomenon near the Al-Fe intermetallic compound at the interface.Zn coating does not participate in intermetallic formation due to its evaporation during WB.It was concluded that the softening of the base metal in the heat-affected zone rather than the IMCs determines the joint efficiency. 展开更多
关键词 weld-brazing Al-steel ternary Al-Fe intermetallic joint efficiency hot cracking
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Friction stir welding of aluminum to copper—An overview 被引量:13
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作者 Nidhi SHARMA Zahid A.KHAN Arshad Noor SIDDIQUEE 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2017年第10期2113-2136,共24页
Components made by joining different materials are required in various engineering applications.Fabrication of suchcomponents is a challenging task due to the vast difference in mechanical,thermal and electrical prope... Components made by joining different materials are required in various engineering applications.Fabrication of suchcomponents is a challenging task due to the vast difference in mechanical,thermal and electrical properties of the materials beingused.Friction stir welding(FSW)is capable of joining dissimilar materials such as aluminum(Al)and copper(Cu)and thereforeresearchers have used this novel process for dissimilar joining.Consequently,several works pertaining to dissimilar joining,specifically Al?Cu,are available in the literature but they are scattered in different sources,which makes the task of gatheringinformation about dissimilar FSW of Al?Cu cumbersome.This work has been written with an aim to provide all pertinentinformation related to dissimilar FSW of Al?Cu at one place to ease the problems of researchers.It comprehensively covers andsummarizes the topics such as the effect of tool design and geometry,FSW process parameters,FSW strategies on mechanicalproperties,microstructure and formation of defects during dissimilar FSW of Al?Cu.In addition,it also presents and discussesseveral variants of dissimilar FSW of Al?Cu.Finally,this work not only puts forth major findings of the previous researchers but alsosuggests future recommendations for dissimilar FSW of Al?Cu. 展开更多
关键词 aluminium COPPER friction stir welding intermetallic compounds mechanical properties weld nugget zone
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Microstructural characterization and mechanical properties of aluminum 6061-T6 plates welded with copper insert plate(Al/Cu/Al) using friction stir welding 被引量:4
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作者 Vahid M.Khojastehnezhad Hamed H.Pourasl 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2018年第3期415-426,共12页
Friction stir welding was used to join two aluminum 6061-T6 plates with an insert of a pure copper plate(Al/Cu/Al),and then the influence of the copper insert on the joint performance was studied.The dissimilar weldin... Friction stir welding was used to join two aluminum 6061-T6 plates with an insert of a pure copper plate(Al/Cu/Al),and then the influence of the copper insert on the joint performance was studied.The dissimilar welding results were also compared with AA 6061 friction stir welds produced without copper insert(Al/Al).Optical and scanning electron microscopes were used for the microstructural observations of the welded samples.X-ray diffraction analysis was used to analyze phase component of the Al/Cu/Al specimen.A defect-free joint was observed for the Al/Cu/Al joint at a rotational speed of 950 r/min and a welding speed of 50 mm/min.Microstructural observation of the weld nugget zone(WNZ)demonstrates the formation of composite-like structure which promotes metallurgical bonding of aluminum and copper.XRD results show the formation of intermetallic compounds(IMCs),such as Al4Cu9 and Al2Cu.Furthermore,it was observed that the hardness of the weld with the Cu insert plate is higher than that of other samples due to more dislocation density and a distinct rise in hardness values was observed due to the presence of IMCs.The ultimate tensile strength of the joint with copper insert plate is higher than that of the other sample due to the strong metallurgical bonding between Al and Cu. 展开更多
关键词 friction stir welding Al/Cu/Al intermetallic compounds microstructure mechanical properties
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TIG welding-brazing joint of aluminum to stainless steel with hot wire 被引量:1
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作者 何欢 林三宝 +2 位作者 陈哲 范成磊 杨春利 《China Welding》 EI CAS 2013年第3期25-30,共6页
TIG welding-brazing process with high frequency induction hot wire technology was presented to create joints between 5A06 aluminum alloy and SUS32! stainless steel using ER1100 filler wire with different temperature. ... TIG welding-brazing process with high frequency induction hot wire technology was presented to create joints between 5A06 aluminum alloy and SUS32! stainless steel using ER1100 filler wire with different temperature. The joints were evaluated by mechanical test and microstructural analyses. The welding procedure using hot fiUer wire (400 ℃ ) significantly increases strength stability by 71% and average value of tensile strength by 30. 8 % of the joints, compared with cold wire. The research of microstructures in interfaces and welded seams reveals that using 400 ℃ hot filler wire can decrease the thickness of intermetallic compounds ( IMCs ) from 6 to 3.5 txm approximately, which is the main reason of mechanical property improvement. 展开更多
关键词 aluminum alloy stainless steel WELDING-BRAZING hot wire high frequency induction INTERMETALLIC
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Preparation of bimetallic nano-composite by dissimilar friction stir welding of copper to aluminum alloy 被引量:10
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作者 Farhad BAKHTIARI ARGESI Ali SHAMSIPUR Seyyed Ehsan MIRSALEHI 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2021年第5期1363-1380,共18页
Butt friction stir welding between pure copper and AA5754 alloy was carried out.Reinforcing SiC nanoparticles were utilized in friction stir welded(FSW)joints to decline the harmful effects of intermetallic compounds.... Butt friction stir welding between pure copper and AA5754 alloy was carried out.Reinforcing SiC nanoparticles were utilized in friction stir welded(FSW)joints to decline the harmful effects of intermetallic compounds.Tensile tests,micro-hardness experiments,scanning electron microscopy and X-ray diffraction analysis were applied to studying the properties of welded joints.The joints with a travel speed of 50 mm/min and a rotation speed of 1000 r/min showed the best results.The presence of nano-sized SiC particles reduced the grain size of aluminum and copper in the stir zone(SZ)from 38.3 and 12.4μm to 12.9 and 5.1μm,respectively.The tensile strength of the joint in the presence of reinforcing SiC nano-particles was~240 MPa,which is~90%of that for the aluminum base.Furthermore,the highest microhardness of the weld zone was significantly increased from HV 160 to HV 320 upon the addition of SiC nano-particles.The results also showed that raising the heat generation in FSW joints increased the amount of Al_(4)Cu_(9) and Al_(2)Cu intermetallic compounds. 展开更多
关键词 friction stir welding dissimilar Al/Cu joint SiC nano-particles intermetallic compounds microstructural characteristics mechanical properties
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达托霉素产生菌玫瑰孢链霉菌NRRL11379接合转移系统的建立与优化 被引量:2
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作者 王航 王文轶 +2 位作者 吴旻 彭冉 王旻 《药物生物技术》 CAS 2014年第3期194-198,共5页
玫瑰孢链霉菌(Streptomyces roseosporus)NRRL11379是一种具有环脂肽结构抗生素-达托霉素(Daptomycin)的产生菌。建立高效稳定的遗传操作系统是研究该链霉菌各功能基因作用以及构建基因工程菌的基础。实验探索了通过电穿孔、接合转移向... 玫瑰孢链霉菌(Streptomyces roseosporus)NRRL11379是一种具有环脂肽结构抗生素-达托霉素(Daptomycin)的产生菌。建立高效稳定的遗传操作系统是研究该链霉菌各功能基因作用以及构建基因工程菌的基础。实验探索了通过电穿孔、接合转移向玫瑰孢链霉菌中转入外源DNA的可能性。以链霉菌(Streptomyces)噬菌体ΦC31所构建的整合型载体pSET152-dptP为出发质粒,玫瑰孢链霉菌NRRL11379新鲜菌丝体作为受体菌,在不同的电场强度下进行电穿孔实验,结果表明:以玫瑰孢链霉菌NRRL11379新鲜菌丝体为受体菌,未获得转化子。以ET12567(PUZ8002,pSET152-dptP)为供体,玫瑰孢链霉菌NRRL11379新鲜菌丝体为受体,选取MS培养基为接合转移培养基,利用属间接合转移将质粒pSET152-dptP转入菌株NRRL11379中。结果表明:当供体大肠杆菌ET12567细胞量和玫瑰孢链霉菌NRRL11379菌丝体量比例为1∶1,MS培养基中MgCl2的浓度为0.01 mol/L,培养20 h后覆盖阿泊拉霉素50μg/mL以及萘啶酮酸50μg/mL时,接合转移频率最高,可达1.75×10-4。通过PCR验证,质粒pSET152-dptP已整合至玫瑰孢链霉菌NRRL11379菌株的染色体上。确定了玫瑰孢链霉菌属间接合转移的最佳条件。 展开更多
关键词 玫瑰孢链霉菌NRRL11379 达托霉素 pSET152 电转化 属间接合转移 优化
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