S100 family proteins are calcium-binding proteins, some of which have been shown to have intracellular and extracellular functions associated with inflammation. The serum concentration of S100A12 has been reported to ...S100 family proteins are calcium-binding proteins, some of which have been shown to have intracellular and extracellular functions associated with inflammation. The serum concentration of S100A12 has been reported to increase in the acute phase of Kawasaki disease. The purpose of this study was to evaluate leukocyte gene expressions of S100 family proteins in the acute phase of Kawasaki disease. Ten paired blood samples were obtained from ten patients with Kawasaki disease in the acute phase and in the convalescent phase. We examined leukocyte expression levels of 18 S100 genes in the acute phase compared with those in the convalescent phase by using quantitative real-time polymerase chain reaction. Significantly elevated expression of seven S100 genes (S100A6, A8, A9, A11, A12, S100P, and S100Z) was observed in the acute phase. Conclusion:Of the upregulated S100 genes, calgranulin members of S100 genes (S100A8, S100A9, and S100A12) were most highly expressed in the acute phase. Only one S100 gene, the S100A13 gene, exhibited a significantly decreased expression level in the acute phase.展开更多
目的研究染矽尘大鼠毒理效应相关差异基因表达谱。方法应用气管暴露法对雄性Wistar大鼠进行二氧化硅（50mg／ml）染尘，对照组大鼠气管内灌注1ml灭菌生理盐水。染尘后第14天处死大鼠，取肺组织。应用Ⅲumina激光共聚焦光纤微珠基因芯片...目的研究染矽尘大鼠毒理效应相关差异基因表达谱。方法应用气管暴露法对雄性Wistar大鼠进行二氧化硅（50mg／ml）染尘，对照组大鼠气管内灌注1ml灭菌生理盐水。染尘后第14天处死大鼠，取肺组织。应用Ⅲumina激光共聚焦光纤微珠基因芯片技术，研究大鼠肺组织差异基因表达谱，采用因注释、富集数据库（thedatabase for annotation，visualization and integrated discovery，DAVID）生物信息学分析工具，对差异基因的基因功能和生物学通路进行富集统计学分析。结果检测出的有效基因共22107个，筛选出染矽尘组与对照组的差异基因共1567个，其中上调基因为765个，下调基因为802个。与毒理效应相关的差异基因有461个，其中上调基因为285个，下调的有176个。Real—timePCR实验验证了血红素加氧酶（HMOX1）、过氧化物歧化酶（SOD1）基因上调的趋势和基因芯片结果是一致的。结论二氧化硅粉尘诱导的大鼠肺组织纤维化中起毒理效应的大量相关基因上调或下调，二氧化硅所致的肺纤维化可能存在复杂的基因网络调控系统，其中，毒理机制是肺纤维化发生发展过程中的重萼一环。展开更多
文摘S100 family proteins are calcium-binding proteins, some of which have been shown to have intracellular and extracellular functions associated with inflammation. The serum concentration of S100A12 has been reported to increase in the acute phase of Kawasaki disease. The purpose of this study was to evaluate leukocyte gene expressions of S100 family proteins in the acute phase of Kawasaki disease. Ten paired blood samples were obtained from ten patients with Kawasaki disease in the acute phase and in the convalescent phase. We examined leukocyte expression levels of 18 S100 genes in the acute phase compared with those in the convalescent phase by using quantitative real-time polymerase chain reaction. Significantly elevated expression of seven S100 genes (S100A6, A8, A9, A11, A12, S100P, and S100Z) was observed in the acute phase. Conclusion:Of the upregulated S100 genes, calgranulin members of S100 genes (S100A8, S100A9, and S100A12) were most highly expressed in the acute phase. Only one S100 gene, the S100A13 gene, exhibited a significantly decreased expression level in the acute phase.
文摘目的研究染矽尘大鼠毒理效应相关差异基因表达谱。方法应用气管暴露法对雄性Wistar大鼠进行二氧化硅（50mg／ml）染尘，对照组大鼠气管内灌注1ml灭菌生理盐水。染尘后第14天处死大鼠，取肺组织。应用Ⅲumina激光共聚焦光纤微珠基因芯片技术，研究大鼠肺组织差异基因表达谱，采用因注释、富集数据库（thedatabase for annotation，visualization and integrated discovery，DAVID）生物信息学分析工具，对差异基因的基因功能和生物学通路进行富集统计学分析。结果检测出的有效基因共22107个，筛选出染矽尘组与对照组的差异基因共1567个，其中上调基因为765个，下调基因为802个。与毒理效应相关的差异基因有461个，其中上调基因为285个，下调的有176个。Real—timePCR实验验证了血红素加氧酶（HMOX1）、过氧化物歧化酶（SOD1）基因上调的趋势和基因芯片结果是一致的。结论二氧化硅粉尘诱导的大鼠肺组织纤维化中起毒理效应的大量相关基因上调或下调，二氧化硅所致的肺纤维化可能存在复杂的基因网络调控系统，其中，毒理机制是肺纤维化发生发展过程中的重萼一环。
