近日,Precision Nutrition发表了文章“L-arginine regulates the differential expression of apoptosis genes in gastric cancer cells”。该研究旨在发现L-精氨酸如何诱导胃癌的细胞死亡。作者用CKK8和流式细胞仪检测在补充有L-精...近日,Precision Nutrition发表了文章“L-arginine regulates the differential expression of apoptosis genes in gastric cancer cells”。该研究旨在发现L-精氨酸如何诱导胃癌的细胞死亡。作者用CKK8和流式细胞仪检测在补充有L-精氨酸的RPMI-1640培养基中培养的SGC-7901细胞的细胞毒性和凋亡率。高通量RNA测序寻找差异表达的基因。京都基因和基因组百科全书和GO基因本体论被用来分析包括DEG的生物过程和路径。结果显示在SGC-7901细胞中,L-精氨酸以剂量和时间依赖的方式降低了细胞的活力。展开更多
Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse tes...Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.展开更多
SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉...SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉(背最长肌)和脂肪(大网膜、小网膜、肠系膜、腹膜后、皮下)等11种器官或组织中SIRT1基因mRNA的表达量进行检测,以探讨SIRT1基因的差异性表达规律。结果表明,绵羊SIRT1基因在所检测的器官或组织中均有表达,且差异极显著(P<0.001)。主要表现在脾脏、肝脏和肾脏中的表达显著高于心脏和肺脏中的表达;深层脂肪组织中的表达高于浅层的皮下脂肪;在背最长肌中也有较高表达。这些差异与绵羊SIRT1基因调节脂质代谢及糖异生有关。有关结果为研究SIRT1基因的功能奠定了科学依据。展开更多
文摘近日,Precision Nutrition发表了文章“L-arginine regulates the differential expression of apoptosis genes in gastric cancer cells”。该研究旨在发现L-精氨酸如何诱导胃癌的细胞死亡。作者用CKK8和流式细胞仪检测在补充有L-精氨酸的RPMI-1640培养基中培养的SGC-7901细胞的细胞毒性和凋亡率。高通量RNA测序寻找差异表达的基因。京都基因和基因组百科全书和GO基因本体论被用来分析包括DEG的生物过程和路径。结果显示在SGC-7901细胞中,L-精氨酸以剂量和时间依赖的方式降低了细胞的活力。
文摘Protein kinase C (PKC) is a family of serine/threonine protein kinases, and its multiple isoforms are expressed in various mammalian tissues. The expressions of PKC α and PKC δ at protein and mRNA level in mouse testis were identified by Western blotting and RT\|PCR. The expression of both PKC isoenzymes in the developing mouse testis was also examined. In testes of mouse at various developmental stages, both the protein and the mRNA of PKC\|α were uniformity; but the PKC\|δ expression occurred in the testes of 3\|week old, perhaps even relatively late in spermatid development. The results suggest that each isoenzyme may have different roles in processing and modulating physiological cellular responses of spermatogenesis.
文摘SIRT1(silent mating type information regulation 2homolog1)是一种具有NAD+依赖性去乙酰化酶活性的转录调节因子,参与能量代谢的调节。利用实时荧光定量PCR技术,对10月龄山西肉用绵羊母本品系去势公羊内脏(肝、肾、脾、肺、心)、肌肉(背最长肌)和脂肪(大网膜、小网膜、肠系膜、腹膜后、皮下)等11种器官或组织中SIRT1基因mRNA的表达量进行检测,以探讨SIRT1基因的差异性表达规律。结果表明,绵羊SIRT1基因在所检测的器官或组织中均有表达,且差异极显著(P<0.001)。主要表现在脾脏、肝脏和肾脏中的表达显著高于心脏和肺脏中的表达;深层脂肪组织中的表达高于浅层的皮下脂肪;在背最长肌中也有较高表达。这些差异与绵羊SIRT1基因调节脂质代谢及糖异生有关。有关结果为研究SIRT1基因的功能奠定了科学依据。
