为了揭示极低频脉冲电场(extremely low frequency pulsed electric field,ELF-PEF)对作物幼苗抗旱性的影响及其机理,采用渗透势为-0.1MPa的PEG-6000溶液对玉米幼苗形成生理干旱,研究了1Hz脉冲电场对渗透胁迫下玉米幼苗生长和自发光子...为了揭示极低频脉冲电场(extremely low frequency pulsed electric field,ELF-PEF)对作物幼苗抗旱性的影响及其机理,采用渗透势为-0.1MPa的PEG-6000溶液对玉米幼苗形成生理干旱,研究了1Hz脉冲电场对渗透胁迫下玉米幼苗生长和自发光子辐射的变化。结果表明,在-0.1 MPa的渗透胁迫下玉米幼苗根系和叶片鲜质量缓慢增长,玉米幼苗种胚的自发光子辐射表现出先增加后减小的趋势,幼苗根系和叶片的自发光子辐射也呈现出波动性的增长趋势;经过脉冲电场处理的玉米幼苗根系和叶片鲜质量始终高于(P<0.05)未经过脉冲电场处理的对照组,玉米种胚的自发光子辐射峰值出现的时间比对照组早(P<0.01),峰值辐射强度也比对照高(P<0.01),玉米幼苗根系和叶片的自发光子辐射都有所增强(P<0.05),在渗透胁迫初期脉冲电场处理对玉米幼苗叶片自发光子辐射的增强效应非常显著(P<0.01)。研究结果为揭示极低频脉冲电场对作物幼苗抗旱性的影响机理提供参考。展开更多
In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO ...In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL-1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.展开更多
Pennisetum centrasiaticum is widely distributed in arid and semi-arid areas of Tibet. Its rhizome system is developed and has strong resistance to adversity. In this study, the physiological characteristics and drough...Pennisetum centrasiaticum is widely distributed in arid and semi-arid areas of Tibet. Its rhizome system is developed and has strong resistance to adversity. In this study, the physiological characteristics and drought resistance of P.centrasiaticum seedlings from 12 drought-stressed sites in Tibet were examined at the Lhasa Plateau Ecosystem Research Station of the Chinese Academy of Sciences. PEG-6000 solution with five levels of water potential(0, –0.7, –1.4, –2.1, and –2.8 MPa) was used to simulate drought stress, and malondialdehyde(MDA), proline(Pro) and chlorophyll contents were determined. The balance between production and elimination of reactive oxygen species in P.centrasiaticum was destroyed, leading to membrane lipid peroxidation and the production of MDA, and accelerating the decomposition of chlorophyll. P.centrasiaticum absorbed water from the outside to resist drought by secreting proline and other osmotic regulating substances. The Pro and chlorophyll contents in P.centrasiaticum showed a temporary rising trend, and then decreased with the decrease in water potential. MDA content increased with the decrease in water potential. By using the membership function method, the drought resistance of P.centrasiaticum seedlings from the 12 areas was evaluated, and the results showed that the drought resistance at the sites went from strong to weak in this order: Xietongmen > Linzhou > Sog > Damxung > Tingri > Namling > Gyirong > Linzhi > Purang > Dingjie > Longzi > Sa’gya. The drought resistance of P.centrasiaticum was strong in Xietongmen, Linzhou and Sog. Whether P.centrasiaticum from these three areas is suitable for cultivation in arid and semi-arid areas of Tibet needs further study.展开更多
文摘为了揭示极低频脉冲电场(extremely low frequency pulsed electric field,ELF-PEF)对作物幼苗抗旱性的影响及其机理,采用渗透势为-0.1MPa的PEG-6000溶液对玉米幼苗形成生理干旱,研究了1Hz脉冲电场对渗透胁迫下玉米幼苗生长和自发光子辐射的变化。结果表明,在-0.1 MPa的渗透胁迫下玉米幼苗根系和叶片鲜质量缓慢增长,玉米幼苗种胚的自发光子辐射表现出先增加后减小的趋势,幼苗根系和叶片的自发光子辐射也呈现出波动性的增长趋势;经过脉冲电场处理的玉米幼苗根系和叶片鲜质量始终高于(P<0.05)未经过脉冲电场处理的对照组,玉米种胚的自发光子辐射峰值出现的时间比对照组早(P<0.01),峰值辐射强度也比对照高(P<0.01),玉米幼苗根系和叶片的自发光子辐射都有所增强(P<0.05),在渗透胁迫初期脉冲电场处理对玉米幼苗叶片自发光子辐射的增强效应非常显著(P<0.01)。研究结果为揭示极低频脉冲电场对作物幼苗抗旱性的影响机理提供参考。
基金supported by the National Natural Science Foundation of China (No. 30771646)Shandong Province Independent Innovation Project with the title of ‘Industrialization development of several special seaweeds biological products using integrated technologies’
文摘In order to determine the role of alginate-derived oligosaccharides (ADO) in drought stress resistance of tomato (Ly-copersicon esculentum Miller) seedlings, the leaves were exposed to different concentrations of ADO (0.05%, 0.10%, 0.20%, 0.30% and 0.50%) after drought stress was simulated by exposing the roots to 0.6 molL-1 PEG-6000 solution for 6 h. Changes in biomass, electrolyte leakage and malondialdehyde (MDA), free proline, total soluble sugars (TSS) and abscisic acid (ABA), the enzyme activities of catalase (CAT), superoxide dismutase (SOD), peroxidase (POD) and phenylalanine ammonia-lyase (PAL) were measured to investigate the effects of ADO treatment. The results showed that the treatment with an ADO concentration of 0.20% exhibited the highest performance of drought stress resistance in the tomato seedlings by decreasing the electrolyte leakage and the concentration of MDA, increasing the contents of free proline, TSS and ABA, and increasing the activities of CAT, SOD, POD and PAL after treatment with ADO. It is suggested that changes in electrolyte leakage, MDA, osmotic solutes, ABA, anti-oxidative enzyme and PAL activities were responsible for the increased drought stress resistance in tomato seedlings. To our best knowledge, this is the first report of the effect of ADO treatment on enhancing the drought stress resistance of tomato seedlings.
基金The National Key Research and Development Program of China(2016YFC0502005)The Tibet Science and Technology Major Projects of the Pratacultural Industry(XZ201901NA03)+1 种基金The Lhasa Industrial Integration Project(XCKJ004)Research and Demonstration on the De-velopment Model of Forage Industry in Dagze County(LSKJ2018006)。
文摘Pennisetum centrasiaticum is widely distributed in arid and semi-arid areas of Tibet. Its rhizome system is developed and has strong resistance to adversity. In this study, the physiological characteristics and drought resistance of P.centrasiaticum seedlings from 12 drought-stressed sites in Tibet were examined at the Lhasa Plateau Ecosystem Research Station of the Chinese Academy of Sciences. PEG-6000 solution with five levels of water potential(0, –0.7, –1.4, –2.1, and –2.8 MPa) was used to simulate drought stress, and malondialdehyde(MDA), proline(Pro) and chlorophyll contents were determined. The balance between production and elimination of reactive oxygen species in P.centrasiaticum was destroyed, leading to membrane lipid peroxidation and the production of MDA, and accelerating the decomposition of chlorophyll. P.centrasiaticum absorbed water from the outside to resist drought by secreting proline and other osmotic regulating substances. The Pro and chlorophyll contents in P.centrasiaticum showed a temporary rising trend, and then decreased with the decrease in water potential. MDA content increased with the decrease in water potential. By using the membership function method, the drought resistance of P.centrasiaticum seedlings from the 12 areas was evaluated, and the results showed that the drought resistance at the sites went from strong to weak in this order: Xietongmen > Linzhou > Sog > Damxung > Tingri > Namling > Gyirong > Linzhi > Purang > Dingjie > Longzi > Sa’gya. The drought resistance of P.centrasiaticum was strong in Xietongmen, Linzhou and Sog. Whether P.centrasiaticum from these three areas is suitable for cultivation in arid and semi-arid areas of Tibet needs further study.
