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光电度盘测微器的数据采集及归算软件设计 被引量:1
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作者 许林生 李志刚 《陕西天文台台刊》 CSCD 1992年第2期165-171,共7页
光电度盘测微器是子午环的重要组成部分之一,根据设计原则,要求整个系统的重复精度优于0.05。即要求测定刻线位置精度优于0.05μm,因此它是高精度的测量装置。介绍此装置的数据采集及归算软件。经一年来的仪器运转证明,此方案是有效的,... 光电度盘测微器是子午环的重要组成部分之一,根据设计原则,要求整个系统的重复精度优于0.05。即要求测定刻线位置精度优于0.05μm,因此它是高精度的测量装置。介绍此装置的数据采集及归算软件。经一年来的仪器运转证明,此方案是有效的,已达到原设计要求。 展开更多
关键词 度盘 测量 微器 数据采集 软件
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High Sensitivity Submicron Scale Temperature Sensor Based on Perovskite Nanoplatelet Lasers
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作者 ZHAO Ruofan TAO Jianxun +7 位作者 XI Yuying CHEN Jiangzhao JI Ting WANG Wenyan WEN Rong CUI Yanxia CHEN Junsheng LI Guohui 《发光学报》 EI CAS CSCD 北大核心 2024年第9期1511-1520,共10页
Submicron scale temperature sensors are crucial for a range of applications,particularly in micro and na-noscale environments.One promising solution involves the use of active whispering gallery mode(WGM)microresonato... Submicron scale temperature sensors are crucial for a range of applications,particularly in micro and na-noscale environments.One promising solution involves the use of active whispering gallery mode(WGM)microresonators.These resonators can be remotely excited and read out using free-space structures,simplifying the process of sensing.In this study,we present a submicron-scale temperature sensor with a remarkable sensitivity up to 185 pm/℃based on a trian-gular MAPbI3 nanoplatelet(NPL)laser.Notably,as temperature changes,the peak wavelength of the laser line shifts lin-early.This unique characteristic allows for precise temperature sensing by tracking the peak wavelength of the NPL laser.The optical modes are confined within the perovskite NPL,which measures just 85 nm in height,due to total internal reflec-tion.Our NPL laser boasts several key features,including a high Q of~2610 and a low laser threshold of about 19.8μJ·cm^(−2).The combination of exceptional sensitivity and ultra-small size makes our WGM device an ideal candidate for integration into systems that demand compact temperature sensors.This advancement paves the way for significant prog-ress in the development of ultrasmall temperature sensors,opening new possibilities across various fields. 展开更多
关键词 temperature sensor submicron scale perovskite nanoplatelet
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The biofilm characteristics and enhanced performance of a marine microbial-electrolysis-cell-based biosensor under positive anodic potential
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作者 CAO Yuanyuan ZHANG Chaoqun +2 位作者 LIU Xiang CHENG Liang YANG Yang 《土木与环境工程学报(中英文)》 CSCD 北大核心 2024年第6期221-230,共10页
Microbial fuel cells have already been used as biosensors to monitor assimilable organic carbon(AOC).However,their signal production from AOC is known to be completely suppressed by dissoved oxygen(DO).In this study,t... Microbial fuel cells have already been used as biosensors to monitor assimilable organic carbon(AOC).However,their signal production from AOC is known to be completely suppressed by dissoved oxygen(DO).In this study,two identical microbial electrolysis cell(MEC)based biosensors were inoculated with marine sediment and operated at two different anodic potentials,namely-300 mV and+250 mV relative to Ag/AgCl.The MEC biosensor operated under positive anodic potential conditions had electrochemically active microbial communities on the anode,including members of the Shewanellaceae,Pseudoalteromonadaceae,and Clostridiaceae families.However,the strictly anaerobic members of the Desulfuromonadaceae,Desulfobulbaceae and Desulfobacteraceae families were found only in the negative anodic potential MEC biosensor.The positive anodic potential MEC biosensor showed several other advantages as well,such as faster start-up,significantly higher maximum current production,fivefold improvement in the AOC detection limit,and tolerance of low dissolved oxygen,compared to those obtained from the negative anodic potential MEC biosensor.The developed positive anodic potential MEC biosensor can thus be used as a real-time and inexpensive detector of AOC concentrations in high saline and low DO seawater. 展开更多
关键词 BIOSENSOR microbial fuel cell anodic potential marine biofilm assimilable organic carbon
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Asymmetrical Fabry-Perot cavity slot micro-ring resonator and its sensing characteristics
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作者 CAO Qianqian LIU Chunjuan +1 位作者 WU Xiaosuo SUN Xiaoli 《Journal of Measurement Science and Instrumentation》 CAS CSCD 2024年第3期292-301,共10页
To achieve high quality factor and high-sensitivity refractive index sensor,a slot micro-ring resonator(MRR)based on asymmetric Fabry-Perot(FP)cavity was proposed.The structure consisted of a pair of elliptical holes ... To achieve high quality factor and high-sensitivity refractive index sensor,a slot micro-ring resonator(MRR)based on asymmetric Fabry-Perot(FP)cavity was proposed.The structure consisted of a pair of elliptical holes to form an FP cavity and a microring resonator.The two different optical modes generated by the micro-ring resonator were destructively interfered to form a Fano line shape,which improved the system sensitivity while obtaining a higher quality factor and extinction ratio.The transmission principle of the structure was analyzed by the transfer matrix method.The transmission spectrum and mode field distribution of the proposed structure were simulated by the finite difference time domain(FDTD)method,and the key structural parameters affecting the Fano line shape in the device were optimized.The simulation results show that the quality factor of the device reached 22037.1,and the extinction ratio was 23.9 dB.By analyzing the refractive index sensing characteristics,the sensitivity of the structure was 354 nm·RIU−1,and the detection limit of the sensitivity was 2×10−4 RIU.Thus,the proposed compact asymmetric FP cavity slot micro-ring resonator has obvious advantages in sensing applications owing to its excellent performance. 展开更多
关键词 micro-ring resonator(MRR) Fabry-Perot(FP)cavity Fano resonance refractive index sensing integrated optics silicon waveguide
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Comparative study on the efficacy of transoral robotic surgery and non-robotic surgery for tongue base tumors
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作者 YU Wenjun LIN Quanquan +2 位作者 FENG Lin ZHANG Haizhong XI Qing 《机器人外科学杂志(中英文)》 2024年第5期952-958,共7页
Objective:To compare the efficacy of transoral robotic surgery(TORS)and non-robotic surgery(NRS)in the treatment of tongue base tumors.Methods:A total of 45 patients with tongue base tumors treated in our hospital wer... Objective:To compare the efficacy of transoral robotic surgery(TORS)and non-robotic surgery(NRS)in the treatment of tongue base tumors.Methods:A total of 45 patients with tongue base tumors treated in our hospital were selected,and they were divided into the TORS group and NRS group according to different surgical methods.The surgical indicators and postoperative complications of patients in the two groups were compared and analyzed.Results:Compared with the NRS group,the operative time,bleeding volume and length of hospital stay were less in the TORS group,and the postoperative recurrence rate was less in the TORS group than that in the NRS group.The incidence rate of dysphagia and restricted mouth opening in the TORS group was lower than that in the NRS group within 30 d after surgery,and the difference was statistically significant(P<0.05).Conclusion:TORS has better minimally invasive advantages in the treatment of tongue base tumors,including less intraoperative bleeding,smaller trauma,shorter length of hospital stay and faster recovery. 展开更多
关键词 Tongue Base Tumor Transoral Robotic Surgery Non-robotic Surgery Minimally Invasive Surgery EFFICACY COMPLICATION
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缩微品计算机管理之设想 被引量:2
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作者 叶根平 《缩微技术》 1998年第4期43-45,共3页
缩微技术出现以来,缩微胶片一直是文档(Documents)高容量存储的主要媒体之一。缩微胶片在存储寿命、法律效能、低成本等方面的长处,为图书馆、档案馆等文档管理部门广泛应用于手稿、图纸、珍善本、图书、资料等特殊文档的... 缩微技术出现以来,缩微胶片一直是文档(Documents)高容量存储的主要媒体之一。缩微胶片在存储寿命、法律效能、低成本等方面的长处,为图书馆、档案馆等文档管理部门广泛应用于手稿、图纸、珍善本、图书、资料等特殊文档的长期存储和保管,积累了大量存储在缩... 展开更多
关键词 缩微品 计算机管理 文献缩微器
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A High Precision CMOS Opamp Suitable for ISFET Readout 被引量:1
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作者 张翀 杨海钢 魏金宝 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2008年第4期686-692,共7页
This paper presents a high precision CMOS opamp suitable for ISFET readout. The opamp is tailored to provide a constant bias condition for ISFET as part of the readout circuits and,hence,is compatible for single chip ... This paper presents a high precision CMOS opamp suitable for ISFET readout. The opamp is tailored to provide a constant bias condition for ISFET as part of the readout circuits and,hence,is compatible for single chip integration with the sensor. A continuous time auto-zero stabilization technique is studied and employed, with the aim of suppressing the low frequency noises, including the offset voltage, 1/f noise, and temperature drift. The design is based on a 0.35μm CMOS process. With a 3.3V power supply,it maintains a DC open loop gain of more than 100dB and an offset voltage of around 11μV,while the overall power dissipation is only 1.48mW. With this opamp, a pH microsensor is constructed, of which the functionality is verified by experimental tests. 展开更多
关键词 high precision auto-zero operational amplifier ISFET microsensor
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Three-dimensional analytical solution of acoustic emission or microseismic source location under cube monitoring network 被引量:15
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作者 董陇军 李夕兵 《Transactions of Nonferrous Metals Society of China》 SCIE EI CAS CSCD 2012年第12期3087-3094,共8页
To find the analytical solution of the acoustic emission/microseismic(AE/MS) source location coordinates, the sensor location coordinates were optimized and simplified. A cube monitoring network of sensor location was... To find the analytical solution of the acoustic emission/microseismic(AE/MS) source location coordinates, the sensor location coordinates were optimized and simplified. A cube monitoring network of sensor location was selected, and the AE/MS source localization equations were established. A location method with P-wave velocity by analytical solutions (P-VAS) was obtained with these equations. The virtual location tests show that the relocation results of analytical method are fully consistent with the actual coordinates for events both inside and outside the monitoring network; whereas the location error of traditional time difference method is between 0.01 and 0.03 m for events inside the sensor array, and the location errors are larger, which is up to 1080986 m for events outside the sensor array. The broken pencil location tests were carried out in the cross section of 100 mm×98 mm, 350 mm-length granite rock specimen using five AE sensors. Five AE sources were relocated with the conventional method and the P-VAS method. For the four events outside monitoring network, the positioning accuracy by P-VAS method is higher than that by the traditional method, and the location accuracy of the larger one can be increased by 17.61 mm. The results of both virtual and broken pencil location tests show that the proposed analytical solution is effective to improve the positioning accuracy. It can locate the coordinates of AE/MS source only using simple four arithmetic operations, without determining the fitting initial value and iterative calculation, which can be solved by a conventional calculator or Microsoft Excel. 展开更多
关键词 acoustic emission seismic source SENSOR LOCATION analytical solution
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Motion mechanism and gait planning of a wheeled micro robot 被引量:1
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作者 于会涛 马培荪 何冬青 《Journal of Southeast University(English Edition)》 EI CAS 2006年第2期191-195,共5页
Based on a novel shape memory alloy (SMA) actuator, a micro worming robot is presented. The robot adopts a wheeled moving mechanism. The principle of the robot's enlarged pace is introduced, and the structure and m... Based on a novel shape memory alloy (SMA) actuator, a micro worming robot is presented. The robot adopts a wheeled moving mechanism. The principle of the robot's enlarged pace is introduced, and the structure and motion mechanism of the SMA actuator and the wheeled moving mechanism are discussed. The gait about the robot's rectilinear movement and turning movement is also planned. Under the effect of the eccentric wheel self-locking mechanisms and changing-direction mechanisms, the robot can move forward and backward, and turn actively, which overcomes the disadvantages of the traditional SMA micro robots to a certain extent. Furthermore, some experiments on the heating current of the SMA actuator and the robot's motion capability are carded out. 展开更多
关键词 micro robot shape memory alloy actuator WHEELED gait planning
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Scanning Electron Microscopy of Antennae of Aphidoletes aphidimyza (Diptera: Cecidomyiidae) 被引量:6
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作者 张洁 杨茂发 《Zoological Research》 CAS CSCD 北大核心 2008年第1期108-112,共5页
The morphology of antennae of Aphidoletes aphidimyza was observed with a scanning electron microscope. The results showed that both male and female were fourteen segmented, the male was approximately 2000 μm and the ... The morphology of antennae of Aphidoletes aphidimyza was observed with a scanning electron microscope. The results showed that both male and female were fourteen segmented, the male was approximately 2000 μm and the female was 1050μm. Six types of sensillae on the antenna were observed, viz. chaetica (Ch), trichoidea (Tr), basiconica (Ba), cavity (Ca), styloid (St) and circumfila (Ci) on the antennae of A. aphidimyza. Sensillae Ch had a long external-process, with a base surrounded by membranous sockets and a length of about 67.5 μm. Sensillae Tr were distally curved and inserted into a depression, 61.0μm long. Sensillae Ba were peg-like and 4.7μm long on the antennae. Sensillae Ca were pit-like in appearance and the diameter of the pit was 1.2μm. Sensilla St was found on the second sub-segment flagellum of the male antennae. The length of the sensilla was about 21 μm and the diameter was 1.5μm. The circurnfila, which are a unique type of sensilla found only on cecidomyiid antennae, formed loops around each of the antennal sub-segments, and were attached to the surface by a series of stalks. Sensilla St was only present on male antenna. The number of Ba and Tr was almost the same in both sexes. There were more Sensilla Ca on the male antenna than on the female, while there was more Ch on the female. 展开更多
关键词 Aphidoletes aphidimyza Scanning electron microscopy ANTENNA Sensillae ULTRASTRUCTURE
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Micro-displacement amplifying mechanism driven by piezoelectric actuator 被引量:10
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作者 马浩全 胡德金 张凯 《Journal of Southeast University(English Edition)》 EI CAS 2004年第1期75-79,共5页
Piezoelectric actuator has high stiffness, high frequency and infinite control precision, but a short output displacement which is often 1/1 000 of its length. In order to meet the requirements that tools feeding shou... Piezoelectric actuator has high stiffness, high frequency and infinite control precision, but a short output displacement which is often 1/1 000 of its length. In order to meet the requirements that tools feeding should be long-travel, high-frequency and high-precision in non-circular precision turning, a new one-freedom flexure hinge structure is put forward to amplify the output displacement of piezoelectric actuator. Theoretical analysis is done on the static and dynamic characteristics of the structure, differential equations are presented, and it is also verified by the finite element method. It's proved by experiments that the output displacement of the structure is 293 μm and its resonant frequency is 312 Hz. 展开更多
关键词 piezoelectric actuator flexure hinge micro displacement amplifying structure
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Fabrication of SiC MESFETs for Microwave Power Applications 被引量:1
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作者 柏松 陈刚 +5 位作者 张涛 李哲洋 汪浩 蒋幼泉 韩春林 陈辰 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2007年第1期10-13,共4页
4H-SiC MESFETs are fabricated on semi-insulating SiC substrates. Key processes are optimized to obtain better device performance. A microwave power amplifier is demonstrated from a 1mm SiC MESFET for S band operation.... 4H-SiC MESFETs are fabricated on semi-insulating SiC substrates. Key processes are optimized to obtain better device performance. A microwave power amplifier is demonstrated from a 1mm SiC MESFET for S band operation. When operated at a drain voltage of 64V, the amplifier shows an output power of 4.09W, a gain of 9.3dB,and a power added efficiency of 31.3%. 展开更多
关键词 4H-SIC MESFET MICROWAVE power amplifier
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AlGaN/GaN HEMTs Power Amplifier MIC with Power Combining at C-Band 被引量:1
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作者 姚小江 李宾 +8 位作者 陈延湖 陈小娟 魏珂 李诚瞻 罗卫军 王晓亮 刘丹 刘果果 刘新宇 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2007年第4期514-517,共4页
A power amplifier MIC with power combining based on AlGaN/GaN HEMTs was fabricated and measured. The amplifier consists of four 10 × 120μm transistors. A Wilkinson splitters and combining were used to divide and... A power amplifier MIC with power combining based on AlGaN/GaN HEMTs was fabricated and measured. The amplifier consists of four 10 × 120μm transistors. A Wilkinson splitters and combining were used to divide and combine the power. By biasing the amplifier at VDS = 40V, IDS = 0.9A, a maximum CW output power of 41.4dBm with a maximum power added efficiency (PAE) of 32.54% and a power combine efficiency of 69% was achieved at 5.4GHz. 展开更多
关键词 AlGaN/GaN HEMTs power combining MIC power amplifiers
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A Generalized Reynolds' Equation For Squeeze-Film Air Damping in MEMS 被引量:5
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作者 鲍敏杭 孙远程 +1 位作者 杨恒 王跃林 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2002年第12期1245-1248,共4页
A differential equation that is generally effective for squeeze film air damping of perforated plate and non perforated plate as well as in MEMS devices is developed.For perforated plate,the thickness and the dimens... A differential equation that is generally effective for squeeze film air damping of perforated plate and non perforated plate as well as in MEMS devices is developed.For perforated plate,the thickness and the dimensions of the plate are not limited.With boundary conditions,pressure distribution and the damping force on the plate can be found by solving the differential equation.Analytical expressions for damping pressure and damping force of a long strip holeplate are presented with a finite thickness and a finite width.To the extreme conditions of very thin plate and very thin hole,the results are reduced to the corresponding results of the conventional Reynolds' equation.Thus, the effectiveness of the generalized differential equation is justified.Therefore,the generalized Reynolds' equation will be a useful tool of design for damping structures in MEMS. 展开更多
关键词 squeeze film air damping MEMS Reynolds' equation
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NEW ROD-SHAPED ULTRASONIC MICROMOTOR AND ITS DRIVING PRINCIPLE 被引量:3
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作者 朱华 董迎晖 +1 位作者 马相林 赵淳生 《Transactions of Nanjing University of Aeronautics and Astronautics》 EI 2006年第1期15-19,共5页
A new rod-shaped traveling wave ultrasonic micromotor is developed. In the micromotor, five pieces of piezoelectric ceramics clamped by two metal cylinders are used as its stator. The driving principle of the rodshape... A new rod-shaped traveling wave ultrasonic micromotor is developed. In the micromotor, five pieces of piezoelectric ceramics clamped by two metal cylinders are used as its stator. The driving principle of the rodshaped ultrasonic motor is simulated. The stator structure and the position to lay these piezoelectric ceramics are calculated to improve the electro mechanical conversion efficiency. A flexible rotor is designed to reduce the radial slip between the stator and the rotor, and to improve the motor efficiency. The prototype motor and its micror driver are tested. The motor is 9 mm in out-diameter, 15 mm in length and 3.2 g in weight. When the motor operates with the first bending frequency (72 kHz) of the stator, its maximal rotational speed and the torque reach 520 r/rain and 4.5 mN · m. Results show that the motor has good stability. The speed fluctuation is controlled within 3% by the frequency automatic tracking technique. 展开更多
关键词 ultrasonic motor micro electro mechanical system MICROMOTOR ACTUATOR
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Nonlinear static characteristics of piezoelectric unimorph bending micro actuators 被引量:1
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作者 康新 董萼良 章定国 《Journal of Southeast University(English Edition)》 EI CAS 2010年第4期603-607,共5页
The nonlinear static characteristic of a piezoelectric unimorph cantilever micro actuator driven by a strong applied electric field is studied based on the couple stress theory.The cantilever actuator consists of a pi... The nonlinear static characteristic of a piezoelectric unimorph cantilever micro actuator driven by a strong applied electric field is studied based on the couple stress theory.The cantilever actuator consists of a piezoelectric layer,a passive(elastic)layer and two electrode layers.First,the nonlinear static characteristic of the actuator caused by the electrostriction of the piezoelectric layer under a strong applied electric field is analyzed using the Rayleigh-Ritz method.Secondly,since the thickness of the cantilever beam is in micro scale and there exists a size effect,the size dependence of the deformation behavior is evaluated using the couple stress theory.The results show that the nonlinearities of the beam deflection increase along with the increase of the applied electric field which means that softening of the micro beam rigidity exists when a strong external electric field is applied.Meanwhile,the optimal value of the thickness ratio for the passive layer and the piezoelectric layer is not around 1.0 which is usually adopted by some previous researchers.Since there exists a size effect of the micro beam deflection,the optimal value of this thickness ratio should be greater than 1.0 in micro scale. 展开更多
关键词 nonlinear static characteristic piezoelectric unimorph micro actuator couple stress theory Rayleigh-Ritz method
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Polysilicon Emitter Double Mesa Microwave P ower SiGe HBT 被引量:3
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作者 刘志农 熊小义 +8 位作者 黄文韬 李高庆 张伟 许军 刘志弘 林惠旺 许平 陈培毅 钱佩信 《Journal of Semiconductors》 EI CAS CSCD 北大核心 2003年第9期897-902,共6页
A new 125mm UHV/CVD SiGe/Si epitaxy equipment SGE500 capable of commercialization is constructed and device-level SiGe HBT material is grown.A polysilicon emitter (PolyE) double mesa microwave power SiGe HBT showing e... A new 125mm UHV/CVD SiGe/Si epitaxy equipment SGE500 capable of commercialization is constructed and device-level SiGe HBT material is grown.A polysilicon emitter (PolyE) double mesa microwave power SiGe HBT showing excellent low current DC characteristics with β=60@V CE/I C=9.0V/300μA,β=100@5V/50mA,BV CBO=22V,f t/f max=5.4GHz/7.7GHz@3V/10mA is demonstrated.The PolyE SiGe HBT needs only 6 lithographical steps and cancels the growth of the thick emitter epitaxy layer,both of which show great potential for volume production.A 60-finger class-A SiGe linear power amplifer (PA) w ith 22dBm of 1dB compress point output power (P 1dB),11dB of power gain (G p) and 26.1% of power added efficiency (PAE) @900MHz,3.5V/0.2A is demonstrated.Another 120-finger class-A SiGe PA with 33.3dBm (2.1W) of P out,10.3dB of G p and 33.9% of PAE @900MHz,11V/0.52A is also demonstrated. 展开更多
关键词 SIGE HBT microwave power amplifer
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The System Design of Semiconductor Device Simulator
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作者 杨廉峰 吴金 +2 位作者 夏君 刘其贵 魏同立 《Journal of Southeast University(English Edition)》 EI CAS 1999年第2期24-29,共6页
This paper studies the numerical simulation for semiconductor devices and discusses the software design of the simulation system. Our focus is on the deep submicron device simulation for BJT, MOSFET, heterojunction bi... This paper studies the numerical simulation for semiconductor devices and discusses the software design of the simulation system. Our focus is on the deep submicron device simulation for BJT, MOSFET, heterojunction bipolar transistors (HBT), etc. So the object oriented technology for software design and its realization is used to make the system easy to implement, maintain and extend. Besides the discussion of simulation and software system design, this paper introduces a device simulator SMDS and its parallel extension under local network environment using CORBA technology. 展开更多
关键词 device simulation submicron device object oriented technology parallel simulation
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Electromagnetic simulation and experimental analysis of microwave heaters for asphalt pavements 被引量:3
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作者 朱松青 张志胜 史泰龙 《Journal of Southeast University(English Edition)》 EI CAS 2011年第4期410-416,共7页
In order to study the thermoelectric efficiency of microwave heating and reproduction of asphalt pavements and the uniformity of reproduction temperature distribution, a waveguide excitation cavity is designed and app... In order to study the thermoelectric efficiency of microwave heating and reproduction of asphalt pavements and the uniformity of reproduction temperature distribution, a waveguide excitation cavity is designed and applied to the structural design of a microwave heater. The structural sizes of the incentive cavities are determined based on the waveguide transmission line theory. Using IE3D software, electromagnetic simulations are respectively carried out in four different situations, including the distances between the magnetron probes (antennas) and a short-circuit board, different horn electric lengths and aperture sizes, different dielectric properties of the asphalt mixture, and the distances between the asphalt surface and the mouth cavity. The results show that, when the distance between the magnetron probe and the short-circuit board is 32.5 ram, it is the best installation site; reduction of aerial length is the main factor in improving the heating uniformity. When the aggregate is limestone, the best heating effect can be produced. Maximum radiation efficiency can be realized by adjusting the space between the heater radiation port and the asphalt pavement. The experimental results of asphalt mixture heating in four different situations have a substantial agreement with the simulation results, which confirms that the developed microwave heater can achieve better impedance matching, thus improving the quality and efficiency of heating regeneration. 展开更多
关键词 microwave heater excitation cavity structural design electromagnetic simulation temperature distribution experimental analysis
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Establishment of a Collection and Detection Technology of Mycoplasma hyopneumoniae in Aerosol 被引量:1
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作者 华利忠 高昆 +4 位作者 武昱孜 白方方 冯志新 刘茂军 邵国青 《Agricultural Science & Technology》 CAS 2013年第7期998-1001,1031,共5页
ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae (Mhp) in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentr... ObjectiveThis study was to establish a simple method for collecting and detecting Mycoplasma hyopneumoniae (Mhp) in aerosol. MethodBased on the mechanisms of liquid impinger and filtration sampler, a double concentration aerosol sampler was designed for collecting Mhp aerosol. Firstly, the collection was performed in a closed environment full of artificial aerosol of Mhp. Secondly, collection efficiency was detected by real-time PCR. Thereafter, the clinical feasibility of the designed equipment was tested by collecting aerosol samples in different pig herds. In one assay, the samples were collected at different times from one pig house challenged with Mhp. In another assay, the samples was collected from the delivery room, nursery and fattenning house of a MPS outbreak farm as well as a Mhp infection positive pig farm without obvious clinical symptoms. All the aerosol samples were then detected by real-time PCR or nested PCR. ResultThe collection efficiency of the designed bioaerosol sampler was (37.04±6.43) %, Mhp could be detected 7 d after intratracheal challenge with pneumonic lung homogenate suspension. Aerosol samples of 11 pig houses from the two Mhp positive pig farms with or without clinical symptoms all showed a positive result of PCR, the positivity rate was 100%. ConclusionA high sensitive collecting and detecting technology of aerosol was successfully established, which can be applied to clinical detection of Mhp in aerosol. 展开更多
关键词 Mycoplasma hyopneumoniae AEROSOL Bioaerosol sampler DETECTION
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