目的:探讨关节腔内注射富血小板血浆联合微骨折处理,对新西兰大白兔膝关节全层透明软骨损伤修复的影响。方法:27只成年雄性新西兰大白兔,体重2.5~3.0 kg,建立右膝关节全层透明软骨缺损模型,平均分为3组,分别为:对照组(CTL组,n = 9)、微...目的:探讨关节腔内注射富血小板血浆联合微骨折处理,对新西兰大白兔膝关节全层透明软骨损伤修复的影响。方法:27只成年雄性新西兰大白兔,体重2.5~3.0 kg,建立右膝关节全层透明软骨缺损模型,平均分为3组,分别为:对照组(CTL组,n = 9)、微骨折组(MF组,n = 9)及关节腔内注射富血小板血浆联合微骨折组(MF@PRP组,n = 9)。术后观察动物一般情况,分别于术后4周、8周及12周取兔右膝关节标本,行大体观察、国际软骨修复协会(International Cartilage Repair Society, ICRS)软骨修复评分、Wakitani评分、苏木精–伊红(Hematoxylin-eosin, HE)染色、过碘酸雪夫(Periodic acid Schiff, PAS)染色等检测。结果:所有实验用兔术后均存活。取材后大体观察及组织学检测可见CTL组、MF组及MF@PRP组中兔膝关节全层软骨缺损得到不同程度的修复,组织学检测可见MF@PRP组中全层软骨缺损处再生组织中可见软骨样细胞及软骨基质,CTL组全层软骨缺损处再生组织主要为纤维软骨。造模后8、12周,MF@PRP组中新西兰大白兔ICRS评分均明显优于CTL组、MF组(P Purpose: To investigate the effect of intra-articular injection of platelet-rich plasma combined with microfracture treatment on the repair of full-thickness articular cartilage defects of knee joint in New Zealand white rabbits. Methods: A total of 27 New Zealand White rabbits (2.5~3.0 kilograms) were used to establish animal model and randomly divided into 3 groups: a control group (n = 9), a microfracture group (n = 9) and a microfracture combined with platelet-rich plasma group (n = 9). To evaluate the repair of articular cartilage defects, macroscopic and histological assessments were performed at 4, 8 and 12 weeks after surgery via macroscopic evaluation, the International Cartilage Repair Society (ICRS) macroscopic scoring system, Wakitani scoring system, hematoxylin-eosin (HE) staining and periodic acid-Schiff (PAS) staining, respectively. Results: All rabbits survived after operation. The results of macroscopic and histological examination showed that the full-thickness articular cartilage defects of the knee joint of rabbits in CTL group, MF group and MF@PRP group were repaired to varying degrees. The histological examination showed that chondroid cells and cartilage matrix were visible in the regenerated tissue of the full-thickness articular cartilage defect in MF@PRP group, while fibrocartilage was the main regenerated tissue of the full-thickness cartilage defect in CTL group. At 8 and 12 weeks after surgery, ICRS scores of New Zealand white rabbits in MF@PRP group were significantly better than those in CTL group and MF group (P < 0.05). At 4, 8 and 12 weeks after surgery, the Wakitani score of New Zealand white rabbits in MF@PRP group was significantly better than that in CTL group and MF group (P < 0.05). Conclusion: Macroscopic and histological evaluation revealed that microfracture combined with serial platelet-rich plasma injection yields better outcomes for the repair of full-thickness articular cartilage defects than microfracture alone.展开更多
文摘目的:探讨关节腔内注射富血小板血浆联合微骨折处理,对新西兰大白兔膝关节全层透明软骨损伤修复的影响。方法:27只成年雄性新西兰大白兔,体重2.5~3.0 kg,建立右膝关节全层透明软骨缺损模型,平均分为3组,分别为:对照组(CTL组,n = 9)、微骨折组(MF组,n = 9)及关节腔内注射富血小板血浆联合微骨折组(MF@PRP组,n = 9)。术后观察动物一般情况,分别于术后4周、8周及12周取兔右膝关节标本,行大体观察、国际软骨修复协会(International Cartilage Repair Society, ICRS)软骨修复评分、Wakitani评分、苏木精–伊红(Hematoxylin-eosin, HE)染色、过碘酸雪夫(Periodic acid Schiff, PAS)染色等检测。结果:所有实验用兔术后均存活。取材后大体观察及组织学检测可见CTL组、MF组及MF@PRP组中兔膝关节全层软骨缺损得到不同程度的修复,组织学检测可见MF@PRP组中全层软骨缺损处再生组织中可见软骨样细胞及软骨基质,CTL组全层软骨缺损处再生组织主要为纤维软骨。造模后8、12周,MF@PRP组中新西兰大白兔ICRS评分均明显优于CTL组、MF组(P Purpose: To investigate the effect of intra-articular injection of platelet-rich plasma combined with microfracture treatment on the repair of full-thickness articular cartilage defects of knee joint in New Zealand white rabbits. Methods: A total of 27 New Zealand White rabbits (2.5~3.0 kilograms) were used to establish animal model and randomly divided into 3 groups: a control group (n = 9), a microfracture group (n = 9) and a microfracture combined with platelet-rich plasma group (n = 9). To evaluate the repair of articular cartilage defects, macroscopic and histological assessments were performed at 4, 8 and 12 weeks after surgery via macroscopic evaluation, the International Cartilage Repair Society (ICRS) macroscopic scoring system, Wakitani scoring system, hematoxylin-eosin (HE) staining and periodic acid-Schiff (PAS) staining, respectively. Results: All rabbits survived after operation. The results of macroscopic and histological examination showed that the full-thickness articular cartilage defects of the knee joint of rabbits in CTL group, MF group and MF@PRP group were repaired to varying degrees. The histological examination showed that chondroid cells and cartilage matrix were visible in the regenerated tissue of the full-thickness articular cartilage defect in MF@PRP group, while fibrocartilage was the main regenerated tissue of the full-thickness cartilage defect in CTL group. At 8 and 12 weeks after surgery, ICRS scores of New Zealand white rabbits in MF@PRP group were significantly better than those in CTL group and MF group (P < 0.05). At 4, 8 and 12 weeks after surgery, the Wakitani score of New Zealand white rabbits in MF@PRP group was significantly better than that in CTL group and MF group (P < 0.05). Conclusion: Macroscopic and histological evaluation revealed that microfracture combined with serial platelet-rich plasma injection yields better outcomes for the repair of full-thickness articular cartilage defects than microfracture alone.
