采用混合模式扩张床吸附介质Streamline Direct HST,直接从牛乳清中分离乳铁蛋白.考察了新型介质的扩张床膨胀特性和流体混合性能,确定合适的操作流速,选定膨胀率为2,可以满足高处理量和床层稳定性的要求.通过静态吸附实验,考察了pH值...采用混合模式扩张床吸附介质Streamline Direct HST,直接从牛乳清中分离乳铁蛋白.考察了新型介质的扩张床膨胀特性和流体混合性能,确定合适的操作流速,选定膨胀率为2,可以满足高处理量和床层稳定性的要求.通过静态吸附实验,考察了pH值和离子强度对乳铁蛋白吸附的影响.结果表明,该介质对乳铁蛋白的吸附容量较大,约110 mg/g介质,具有一定的耐盐特性;改变离子强度不能实现有效洗脱,改变pH值是洗脱的合适途径;进行了扩张床吸附分离,采用pH值阶跃洗脱,从牛乳清中分离得到不同乳清分离蛋白组分,20 mmol/L NaOH洗脱下得到乳铁蛋白,收率为87.8%,纯化因子高达10.5,分离过程体现了扩张床吸附的集成化优势.展开更多
The biomass pulse-response method was established and the biomass transmission index was introduced as a quantitative parameter for evaluating the biomass-adsorbent interactions in the expanded bed.With baker’s yeast...The biomass pulse-response method was established and the biomass transmission index was introduced as a quantitative parameter for evaluating the biomass-adsorbent interactions in the expanded bed.With baker’s yeast intact cells and cell debris as the model biomass and anion exchanger Streamline DEAE as the model adsorbent, a series of factors were investigated to optimize the evaluation method.The appropriate operation conditions were chosen as the OD 600 of biomass pulse at the range of 0.5—0.6, and pulse loading at 80% volume of sedimented bed, expansion factor at 2.5.The method was then used to study the influence of ionic strength of fluid phase on the biomass-adsorbent interactions.The consistent results and slight measurement errors demonstrated that the biomass pulse-response method established in the present work is feasible, reliable and effective for quantitative evaluation of biomass-adsorbent interactions in the expanded bed.展开更多
丹麦Upfront ChromatographyA／S公司Biomine部门引入了一种Rhobust乳清精制系统，即一种从乳清中优选高经济价值功能蛋白产品的新型、经济节省的大型色谱法。据Upfront Chromatography公司透露，该分离系统将扩张床吸附（Expanded Bed ...丹麦Upfront ChromatographyA／S公司Biomine部门引入了一种Rhobust乳清精制系统，即一种从乳清中优选高经济价值功能蛋白产品的新型、经济节省的大型色谱法。据Upfront Chromatography公司透露，该分离系统将扩张床吸附（Expanded Bed Adsorption，EBA）色谱法与已经可行的、先进的膜净化技术相结合，是一种柔性、可持续和经济节省的分离工艺，仅需最低的水耗量就可提取高功能、高质量的蛋白产物。展开更多
基金国家自然科学基金 (No 2 0 2 0 60 2 9)教育部留学回国人员科研启动基金资助项目~~
文摘The biomass pulse-response method was established and the biomass transmission index was introduced as a quantitative parameter for evaluating the biomass-adsorbent interactions in the expanded bed.With baker’s yeast intact cells and cell debris as the model biomass and anion exchanger Streamline DEAE as the model adsorbent, a series of factors were investigated to optimize the evaluation method.The appropriate operation conditions were chosen as the OD 600 of biomass pulse at the range of 0.5—0.6, and pulse loading at 80% volume of sedimented bed, expansion factor at 2.5.The method was then used to study the influence of ionic strength of fluid phase on the biomass-adsorbent interactions.The consistent results and slight measurement errors demonstrated that the biomass pulse-response method established in the present work is feasible, reliable and effective for quantitative evaluation of biomass-adsorbent interactions in the expanded bed.
文摘丹麦Upfront ChromatographyA／S公司Biomine部门引入了一种Rhobust乳清精制系统，即一种从乳清中优选高经济价值功能蛋白产品的新型、经济节省的大型色谱法。据Upfront Chromatography公司透露，该分离系统将扩张床吸附（Expanded Bed Adsorption，EBA）色谱法与已经可行的、先进的膜净化技术相结合，是一种柔性、可持续和经济节省的分离工艺，仅需最低的水耗量就可提取高功能、高质量的蛋白产物。
