AIM: To investigate the expression of transcription factors Slug in human lens epithelial cells(HLECs)undergoing epithelial-mesenchymal transition(EMT)induced by connective tissue growth factor(CTGF).·METHODS: HL...AIM: To investigate the expression of transcription factors Slug in human lens epithelial cells(HLECs)undergoing epithelial-mesenchymal transition(EMT)induced by connective tissue growth factor(CTGF).·METHODS: HLECs were treated with CTGF of different concentrations(20, 50 and 100 ng/m L) or without CTGF(control) for 24 h. The morphological changes of HLECs were analysed by microscopy. The expression and cellular localization of Slug was evaluated by immumo-fluorescence. Expressions of Slug, E-cadherin and alpha smooth muscle actin(α-SMA) were further determined by Western blot analysis.·RESULTS: HLECs showed spidle fibrolasts-like characteristics and loosely connected each other after CTGF treatment. The immuno-fluorescence staining indicated that Slug was localized in the nuclei and its expression was induced by CTGF. The relative expressions of Slug protein were 1.64 ±0.11, 1.96 ±0.03,3.12 ±0.10, and 4.08 ±0.14, respectively, in response to control group and treatment with CTGF of 20, 50 and100 ng/m L(F =443.86, P <0.01). The increased Slug protein levels were correlated well with up-expression of α-SMA(0.78±0.05, 0.85±0.06, 2.17±0.15, 2.86±0.10; F =449.85, P <0.01) and down-expression of E-cadherin(2.50 ±0.11,1.79±0.26, 1.05±0.14, 0.63±0.08; F =101.55, P <0.01).·CONCLUSION: Transcription factor Slug may be involved in EMT of HLECs induced by CTGF in vitro.展开更多
基金Supported by National Natural Science Foundation of China(No.81470614,No.81460163,No.81300786)Fundamental Research Funds for the Central Universities(No.xjj2014146)+1 种基金Specialized Research Fund for the Doctoral Program of Higher Education(No.20133601120012)Key International Communication Project of Shaanxi province(No.2012KW-31)
文摘AIM: To investigate the expression of transcription factors Slug in human lens epithelial cells(HLECs)undergoing epithelial-mesenchymal transition(EMT)induced by connective tissue growth factor(CTGF).·METHODS: HLECs were treated with CTGF of different concentrations(20, 50 and 100 ng/m L) or without CTGF(control) for 24 h. The morphological changes of HLECs were analysed by microscopy. The expression and cellular localization of Slug was evaluated by immumo-fluorescence. Expressions of Slug, E-cadherin and alpha smooth muscle actin(α-SMA) were further determined by Western blot analysis.·RESULTS: HLECs showed spidle fibrolasts-like characteristics and loosely connected each other after CTGF treatment. The immuno-fluorescence staining indicated that Slug was localized in the nuclei and its expression was induced by CTGF. The relative expressions of Slug protein were 1.64 ±0.11, 1.96 ±0.03,3.12 ±0.10, and 4.08 ±0.14, respectively, in response to control group and treatment with CTGF of 20, 50 and100 ng/m L(F =443.86, P <0.01). The increased Slug protein levels were correlated well with up-expression of α-SMA(0.78±0.05, 0.85±0.06, 2.17±0.15, 2.86±0.10; F =449.85, P <0.01) and down-expression of E-cadherin(2.50 ±0.11,1.79±0.26, 1.05±0.14, 0.63±0.08; F =101.55, P <0.01).·CONCLUSION: Transcription factor Slug may be involved in EMT of HLECs induced by CTGF in vitro.
