目的：提高对韦格纳肉芽肿（WG）并发高血糖症的认识。方法结合一例 WG 并发高血糖症患者的临床资料进行文献复习，对 WG 和高血糖症的内在联系进行分析。结果患者入院后在 CT 引导下行肺活检，病理检查结果提示：肉芽肿性炎，高度考虑...目的：提高对韦格纳肉芽肿（WG）并发高血糖症的认识。方法结合一例 WG 并发高血糖症患者的临床资料进行文献复习，对 WG 和高血糖症的内在联系进行分析。结果患者入院后在 CT 引导下行肺活检，病理检查结果提示：肉芽肿性炎，高度考虑为 WG。查胞浆型抗中性粒细胞胞浆抗体（cANCA）：阳性，核周型 ANCA（pANCA）：阴性，抗髓过氧化物酶抗体＜2．0，抗蛋白酶3抗体144．80。入院后监测血糖发现，餐前餐后血糖水平均明显高于正常，经激素＋环磷酰胺＋复方新诺明控制 WG 病情后血糖恢复正常。结论 WG 并发高血糖症原因可能为 WG 累及胰腺导致胰岛β细胞损伤、胰岛素内源性分泌减少。展开更多
Abstract:Objective To map the epitopes on Wegener's granulomatosis autoantigen proteinase 3.Methods Antigenicity of proteinase 3 was studied with Western blot analysis in which proteinase 3 was prepared under redu...Abstract:Objective To map the epitopes on Wegener's granulomatosis autoantigen proteinase 3.Methods Antigenicity of proteinase 3 was studied with Western blot analysis in which proteinase 3 was prepared under reducing and non-reducing conditions. Two anti-proteinase 3 monoclonal antibodies, HZ1F12 and HZ1H3, were used to inhibit each other and to inhibit 22 anti-proteinase 3 positive sera from patients with Wegener's granulomatosis in competitive inhibition enzyme-linked immunosorbent assays (ELISA) and Western blot analysis. Results All monoclonal antibodies and patient sera recognized proteinase 3 under non-reducing conditions in Western blot analysis. HZ1F12 was inhibited 74% by HZ1H3. 10/22 (46%) sera were completely or partially inhibited by HZ1F12; 9/22 (41%) sera were partially inhibited by HZ1H3; and 6/22 (27%) were inhibited by both monoclonal antibodies. In inhibition Western blot analysis, the binding of patient sera to proteinase 3 could be inhibited by HZ1F12. Conclusions The epitopes of Wegener's granulomatosis autoantigen were conformational. Anti-proteinase 3 monoclonal HZ1F12 and HZ1H3 recognized similar or overlapping epitopes on the proteinase 3 molecule. Epitopes of proteinase 3 recognized by anti-proteinase 3 positive sera were not restricted.展开更多
文摘目的：提高对韦格纳肉芽肿（WG）并发高血糖症的认识。方法结合一例 WG 并发高血糖症患者的临床资料进行文献复习，对 WG 和高血糖症的内在联系进行分析。结果患者入院后在 CT 引导下行肺活检，病理检查结果提示：肉芽肿性炎，高度考虑为 WG。查胞浆型抗中性粒细胞胞浆抗体（cANCA）：阳性，核周型 ANCA（pANCA）：阴性，抗髓过氧化物酶抗体＜2．0，抗蛋白酶3抗体144．80。入院后监测血糖发现，餐前餐后血糖水平均明显高于正常，经激素＋环磷酰胺＋复方新诺明控制 WG 病情后血糖恢复正常。结论 WG 并发高血糖症原因可能为 WG 累及胰腺导致胰岛β细胞损伤、胰岛素内源性分泌减少。
文摘Abstract:Objective To map the epitopes on Wegener's granulomatosis autoantigen proteinase 3.Methods Antigenicity of proteinase 3 was studied with Western blot analysis in which proteinase 3 was prepared under reducing and non-reducing conditions. Two anti-proteinase 3 monoclonal antibodies, HZ1F12 and HZ1H3, were used to inhibit each other and to inhibit 22 anti-proteinase 3 positive sera from patients with Wegener's granulomatosis in competitive inhibition enzyme-linked immunosorbent assays (ELISA) and Western blot analysis. Results All monoclonal antibodies and patient sera recognized proteinase 3 under non-reducing conditions in Western blot analysis. HZ1F12 was inhibited 74% by HZ1H3. 10/22 (46%) sera were completely or partially inhibited by HZ1F12; 9/22 (41%) sera were partially inhibited by HZ1H3; and 6/22 (27%) were inhibited by both monoclonal antibodies. In inhibition Western blot analysis, the binding of patient sera to proteinase 3 could be inhibited by HZ1F12. Conclusions The epitopes of Wegener's granulomatosis autoantigen were conformational. Anti-proteinase 3 monoclonal HZ1F12 and HZ1H3 recognized similar or overlapping epitopes on the proteinase 3 molecule. Epitopes of proteinase 3 recognized by anti-proteinase 3 positive sera were not restricted.
