期刊文献+
共找到5篇文章
< 1 >
每页显示 20 50 100
抗甲肝病毒基因工程单抗分离纯化与鉴定(英文)
1
作者 魏敬双 陶苒 +3 位作者 孙巍巍 贾茜 李川 梁米芳 《生物工程学报》 CAS CSCD 北大核心 2004年第2期257-261,共5页
为克服血源免疫球蛋白制品的不足 ,开发了抗甲肝病毒基因工程单克隆抗体anti_HAVIgG。用无血清培养基培养rCHO工程细胞株 ,上清液经过rProteinASFF亲和层析→脱盐→离子交换层析→超滤换液纯化后 ,所得anti_HAVIgG纯度达 99%以上 ,比活... 为克服血源免疫球蛋白制品的不足 ,开发了抗甲肝病毒基因工程单克隆抗体anti_HAVIgG。用无血清培养基培养rCHO工程细胞株 ,上清液经过rProteinASFF亲和层析→脱盐→离子交换层析→超滤换液纯化后 ,所得anti_HAVIgG纯度达 99%以上 ,比活性约 10 0IU mg ,anti_HAVIgG活性回收率 40 %。所纯化的anti_HAVIgG分子量150kD ,等电点 8 4~ 9 3。免疫印迹实验证实anti_HAVIgG为人源全抗体分子。亲和层析介质rProteinASFF确实存在亲和配基脱落问题 ,但通过后续纯化步骤可有效除去。在亲和层析过程中加入高盐清洗步骤 ,可有效降低宿主DNA残留量水平。对样品中自由巯基含量进行了测定 ,认为非还原电泳图谱中低分子量条带是由于抗体分子内存在自由巯基引起。 展开更多
关键词 甲肝病毒单克隆抗体 基因工程 分离 纯化 鉴定 亲和层析 rProtein A残留量
下载PDF
Detection of anti-HAV antibody with dot immunogold filtration assay 被引量:6
2
作者 Zhong-JunShao De-ZhongXu +4 位作者 Yong-PingYan Jing-HuaLi Jing-XiaZhang Zhi-YingZhang Bo-RongPan 《World Journal of Gastroenterology》 SCIE CAS CSCD 2003年第7期1508-1511,共4页
AIM: To establish a rapid, sensitive and specific immunogold assay for detection of hepatitis A virus infection.METHODS: Rabbit monoclonal antibodies to anti-human IgM and IgG (Dako) were dotted on a nitrocellulose me... AIM: To establish a rapid, sensitive and specific immunogold assay for detection of hepatitis A virus infection.METHODS: Rabbit monoclonal antibodies to anti-human IgM and IgG (Dako) were dotted on a nitrocellulose membrane (NCM) respectively to capture the human sera IgM and IgG. Then the captured antibodies would conjugate to HAV antigen, which was revealed by mouse anti-HAV IgG conjugated to gold particles. Final results were assessed by blind method.RESULTS: Sera from 96 patients with acute hepatitis were used for our study. Compared with well-recognized standard (Abbott Laboratory, USA), the sensitivity and specificity of IgM-DIGFA (self-made) were 91.3 % (42/46) and 96.0 %(48/50), and those of IgM-ELISA (Kehua, Shanghai) were 97.8 % (45/46) and 100.0 % (50/50). The identical results were produced from the study with reagents at different conditions, and the study was repeated in 15 negative sera and 10 positive sera. The serum anti-HAV IgG was tested with DIGFA at the same time. In comparison with ELISA,the sensitivity and specificity of DIGFA for IgG anti-HAV were 87.2 % (41/47) and 91.8 % (45/49), respectively.CONCLUSION: This assay can detect anti-HAV IgM and IgG simultaneously, and be done within 3 minutes. The simplicity, rapidity and specificity of the assay were useful for screening and epidemiological study. 展开更多
关键词 甲肝病毒抗体 免疫球蛋白G 免疫球蛋白M 酶链免疫吸附实验 聚合酶链反应
下载PDF
Prevalence of anti-HAV antibodies in multitransfused patients with beta-thalassemia
3
作者 Dimitrios Siagris Alexandra Kouraklis-Symeonidis +7 位作者 Irini Konstantinidou Myrto Christofidou Ioannis Starakis Alexandra Lekkou Christos Papadimitriou Alexandros Blikas Nicholas Zoumbos Chryssoula Labropoulou-Karatza 《World Journal of Gastroenterology》 SCIE CAS CSCD 2008年第10期1559-1563,共5页
AIM: To detect the prevalence of anti-HAV IgG antibodies in adult multitransfused beta-thalassemic patients. METHODS: We studied 182 adult beta-thalassemic patients and 209 controls matched for age and sex from the sa... AIM: To detect the prevalence of anti-HAV IgG antibodies in adult multitransfused beta-thalassemic patients. METHODS: We studied 182 adult beta-thalassemic patients and 209 controls matched for age and sex from the same geographic area, at the same time. Anti-HAV IgG antibodies, viral markers of hepatitis B virus (HBV) and hepatitis C virus (HCV) infection were evaluated. RESULTS: Anti-HAV IgG antibodies were detected more frequently in thalassemic patients (133/182; 73.1%) than in healthy controls (38/209; 18.2%, P < 0.0005). When we retrospectively evaluated the prevalence of anti-HAV IgG antibodies in 176/182 (96.7%) thalassemic patients, whose medical history was available for the previous ten years, it was found that 83 (47.2%) of them were continuously anti-HAV IgG positive, 16 (9.1%) acquired anti-HAV IgG antibody during the previous ten years, 49 (27.8%) presented anti-HAV positivity intermittently and 28 (15.9%) were anti-HAV negative continuously. CONCLUSION: Multitransfused adult beta-thalassemic patients present higher frequency of anti-HAV IgG antibodies than normal population of the same geographic area. This difference is difficult to explain, but it can be attributed to the higher vulnerability of thalassemics to HAV infection and to passive transfer of anti-HAV antibodies by blood transfusions. 展开更多
关键词 Hepatitis A virus Anti-HAV antibodies BETA-THALASSEMIA Multiple transfusions Hepatitis C virus
下载PDF
甲型肝炎灭活疫苗及减毒活疫苗对不同人群免疫效果的研究 被引量:10
4
作者 张秀春 韩莉莉 +3 位作者 邢玉兰 周绍莲 陈长顺 刘明霞 《中国计划免疫》 2002年第5期254-257,共4页
为了对不同种类甲型肝炎 (甲肝 )疫苗的免疫效果进行系统观察 ,确定最佳免疫方案 ,在北京市顺义区及北京铁路局系统 ,采用流行病学试验方法对 4~ 6岁儿童和 13~ 18岁青少年进行了观察。观察对象随机分组 ,分别用甲肝减毒活疫苗和甲肝... 为了对不同种类甲型肝炎 (甲肝 )疫苗的免疫效果进行系统观察 ,确定最佳免疫方案 ,在北京市顺义区及北京铁路局系统 ,采用流行病学试验方法对 4~ 6岁儿童和 13~ 18岁青少年进行了观察。观察对象随机分组 ,分别用甲肝减毒活疫苗和甲肝灭活疫苗 (72 0 EU、36 0 EU)按不同程序进行免疫 ,于免疫后 2周~ 2 4个月采血检测甲肝病毒IgG抗体 (抗 HAV IgG)。结果显示 :抗 HAV IgG在免疫后 6~ 9个月达高峰 ;免疫后 9~ 2 4个月儿童组抗体几何平均滴度 (GMT)变化不大 ,青少年组抗体GMT大幅度下降 ;甲肝减毒活疫苗免疫 6个月时加强免疫 1针组 ,抗体阳转率及GMT均优于未加强免疫组 ;甲肝灭活疫苗 36 0 EU3针 (0、1、6个月 )组与 72 0 EU2针 (0、6个月 )组 ,近期36 0 EU组抗体阳转率及GMT明显优于 72 0 EU组 ,远期两组差异无显著的统计学意义。甲肝减毒活疫苗免疫后 2周抗体阳转率及GMT显著高于甲肝灭活疫苗。甲肝减毒活疫苗及灭活疫苗均有较好的免疫效果 ;0、6个月注射 2针甲肝减毒活疫苗的免疫效果明显优于仅注射 1针组 ;0、1、6个月注射 3针 36 0 EU 与 0、6个月注射 2针 72 0 EU甲肝灭活疫苗 ,两者免疫效果的差异无显著的统计学意义。 展开更多
关键词 灭活疫苗 减毒活疫苗 免疫效果 甲型肝炎 抗甲肝抗体
原文传递
甲型肝炎疫苗及其免疫策略 被引量:9
5
作者 庄辉 《中华流行病学杂志》 CAS CSCD 北大核心 2007年第1期1-4,共4页
甲型肝炎(甲肝)呈世界性分布,按其流行强度,全世界可分为5类流行地区(表1)。据1992—1995年全国病毒性肝炎流行病学调查结果表明,我国广大城市地区10岁儿童的抗甲肝病毒抗体(抗.HAV)流行率在40%以下,属中或低地方性流行地... 甲型肝炎(甲肝)呈世界性分布,按其流行强度,全世界可分为5类流行地区(表1)。据1992—1995年全国病毒性肝炎流行病学调查结果表明,我国广大城市地区10岁儿童的抗甲肝病毒抗体(抗.HAV)流行率在40%以下,属中或低地方性流行地区;但农村地区10岁儿童的抗-HAV流行率多在50%以上,属高地方性流行地区。 展开更多
关键词 甲型肝炎疫苗 免疫策略 甲肝病毒抗体 世界性分布 流行地区 病毒性肝炎 -HAV 流行强度
原文传递
上一页 1 下一页 到第
使用帮助 返回顶部