AIM： To develop a serum or histological marker for early discovery of gastric atrophy or intestinal metaplasia. METHODS： This study enrolled 44 patients with gastric adenocarcinoma, 52 patients with duodenal ulcer, ...AIM： To develop a serum or histological marker for early discovery of gastric atrophy or intestinal metaplasia. METHODS： This study enrolled 44 patients with gastric adenocarcinoma, 52 patients with duodenal ulcer, 14 patients with gastric ulcer and 42 consecutive healthy adults as controls. Each patient received an endoscopy and five biopsy samples were obtained. The degrees of histological parameters of gastritis were categorized following the Updated Sydney System. Anti-parietal cell antibodies （APCA） and anti- Helicobacter pylori （ H pylori） antibodies （AHPA） were analyzed by immunoassays. Hpyloriinfection was diagnosed by rapid unease test and histological examination. RESULTS： Patients with gastric cancer and gastric ulcer are significantly older than healthy subjects, while also displaying higher frequency of APCA than healthy controls. Patients with positive APCA showed higher scores in gastric atrophy and intestinal metaplasia of corpus than patients with negative APCA. Patients with positive AHPA had higher scores in gastric atrophy, intestinal metaplasia, and gastric inflammation of antrum than those patients with negative AHPA. Elderly patients had greater prevalence rates of APCA. Following multivariant logistic regression analysis, the only significant risk factor for antral atrophy is positive AHPA, while that for corpus atrophy is positive APCA. CONCLUSION： The existence of positive APCA correlates with glandular atrophy in corpus and the presence of positive AHPA correlates with glandular atrophy in antrum. The existence of serum APCA and AHPA betokens glandular atrophy and requires further examination for gastric cancer.展开更多
Objective: To study the effect of surface configuration on the corrosion resistance and bacterium accumulation of pure Titanium(Ti) when used in oral environment. Methods: Six edentulous volunteers with healthy oral m...Objective: To study the effect of surface configuration on the corrosion resistance and bacterium accumulation of pure Titanium(Ti) when used in oral environment. Methods: Six edentulous volunteers with healthy oral mucosa participated in an in vivo study. Two kinds of pure Ti testing pieces with plane and wrinkly surface were fixed in the polished surface of upper complete dentures. After 6-month wearing, dynamic polarization curves were traced with electrochemical method and the amount and species of bacterium adhered on pure Ti were examined. Results: ① Ep and Ip of specimen in oral cavity was higher than that left in air, which meant corrosion resistance falling. Compared to plane one, Ecorr of wrinkly specimen was more positive and Ep and Ip were more higher, so its corrosion resistance reduced. ② Individual difference put a significant influence on amount of bacterium adhered on pure Titanium, but had no relation to species of bacterium. To the same patient, wrinkly samples collected more bacterium than plane ones, and exhibited G - coccus beside G + coccus. Conclusion: From the perspective of corrosion behavior and benefit to periodontal tissue, wrinkly surface should not be adopted when pure Ti prosthesis is made,especially on connector part of denture.展开更多
Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), a...Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).展开更多
The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles...The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles which was born after identification and characterization. The follow-up of the kinetics of growth of B. breve in the milk enriched by various concentrations of Spirulina （0, 1, 5 and 10 mg/mL） was realized, and the authors estimated the antimicrobial effect of the cultures realised on three pathogenic bacteria： a bacteria isolated from saddles ＂Salmonella ssp.＂, two reference stumps ＂Staphylococcus aureus ATCC25923＂ and ＂Escherichia coli ATCC25922＂. The growth rate was 0.11 hl for the culture of B. breve associated with Spirulina （10 mg/mL）, 0.09 hl for the culture of B. breve enriched with Spirulina （5 mg/mL） and 0.08 hl for the culture of B. breve enriched with Spirulina （1 mg/mL）. In the case of the pure culture of B. breve, the growth rate was 0.07 h1. The authors observed inhibitory effects of B. breve in the various cultures towards the considered pathogenic bacteria. The optimal antimicrobial activity was observed in the preculture bifid with the algae culture （10 mg/mL） towards Salmonella ssp. （Zi = 25 mm）, S. aureus ATCC25923 （Zi = 26 mm） and E. coli ATCC25922 （Zi = 28 mm）.展开更多
基金Supported by Research Grant VGHKS-92-20 and VGHKS-93-28from Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, China
文摘AIM： To develop a serum or histological marker for early discovery of gastric atrophy or intestinal metaplasia. METHODS： This study enrolled 44 patients with gastric adenocarcinoma, 52 patients with duodenal ulcer, 14 patients with gastric ulcer and 42 consecutive healthy adults as controls. Each patient received an endoscopy and five biopsy samples were obtained. The degrees of histological parameters of gastritis were categorized following the Updated Sydney System. Anti-parietal cell antibodies （APCA） and anti- Helicobacter pylori （ H pylori） antibodies （AHPA） were analyzed by immunoassays. Hpyloriinfection was diagnosed by rapid unease test and histological examination. RESULTS： Patients with gastric cancer and gastric ulcer are significantly older than healthy subjects, while also displaying higher frequency of APCA than healthy controls. Patients with positive APCA showed higher scores in gastric atrophy and intestinal metaplasia of corpus than patients with negative APCA. Patients with positive AHPA had higher scores in gastric atrophy, intestinal metaplasia, and gastric inflammation of antrum than those patients with negative AHPA. Elderly patients had greater prevalence rates of APCA. Following multivariant logistic regression analysis, the only significant risk factor for antral atrophy is positive AHPA, while that for corpus atrophy is positive APCA. CONCLUSION： The existence of positive APCA correlates with glandular atrophy in corpus and the presence of positive AHPA correlates with glandular atrophy in antrum. The existence of serum APCA and AHPA betokens glandular atrophy and requires further examination for gastric cancer.
文摘Objective: To study the effect of surface configuration on the corrosion resistance and bacterium accumulation of pure Titanium(Ti) when used in oral environment. Methods: Six edentulous volunteers with healthy oral mucosa participated in an in vivo study. Two kinds of pure Ti testing pieces with plane and wrinkly surface were fixed in the polished surface of upper complete dentures. After 6-month wearing, dynamic polarization curves were traced with electrochemical method and the amount and species of bacterium adhered on pure Ti were examined. Results: ① Ep and Ip of specimen in oral cavity was higher than that left in air, which meant corrosion resistance falling. Compared to plane one, Ecorr of wrinkly specimen was more positive and Ep and Ip were more higher, so its corrosion resistance reduced. ② Individual difference put a significant influence on amount of bacterium adhered on pure Titanium, but had no relation to species of bacterium. To the same patient, wrinkly samples collected more bacterium than plane ones, and exhibited G - coccus beside G + coccus. Conclusion: From the perspective of corrosion behavior and benefit to periodontal tissue, wrinkly surface should not be adopted when pure Ti prosthesis is made,especially on connector part of denture.
基金Supported by the Ministry of Science and Technology(No.2010CB833802)the Knowledge Innovation Program of Chinese Academy of Sciences(No.KSCX2-EW-G-12B)the National Natural Science Foundation of China(No.30910103914)
文摘Bioassay-guided fractionation of the crude extract from Penicillium commune SD-118, a fungus obtained from a deep-sea sediment sample, resulted in the isolation of a known antibacterial compound, xanthocillin X (1), and 14 other known compounds comprising three steroids (2-4), two ceramides (5 and 6), six aromatic compounds (7-12), and three alkaloids (13-15). Xanthocillin X (1) was isolated for the first time from a marine fungus. In the bioassay, xanthocillin X (1) displayed remarkable antimicrobial activity against Staphylococcus aureus and Escherichia coli, and significant cytotoxicity against MCF-7, HepG2, H460, Hela, Du145, and MDA-MB-231 cell lines. Meleagrin (15) exhibited cytotoxicity against HepG2, Hela, Du145, and MDA-MB-231 cell lines. This is the first report of the cytotoxicity of xanthocillin X (1).
文摘The aim of this study is to demonstrate the influence of Spirulina platensis on the growth of Bifidobacteium breve in the infantile milk. The authors used a stump of Bifidobacterium breve isolated from the new saddles which was born after identification and characterization. The follow-up of the kinetics of growth of B. breve in the milk enriched by various concentrations of Spirulina （0, 1, 5 and 10 mg/mL） was realized, and the authors estimated the antimicrobial effect of the cultures realised on three pathogenic bacteria： a bacteria isolated from saddles ＂Salmonella ssp.＂, two reference stumps ＂Staphylococcus aureus ATCC25923＂ and ＂Escherichia coli ATCC25922＂. The growth rate was 0.11 hl for the culture of B. breve associated with Spirulina （10 mg/mL）, 0.09 hl for the culture of B. breve enriched with Spirulina （5 mg/mL） and 0.08 hl for the culture of B. breve enriched with Spirulina （1 mg/mL）. In the case of the pure culture of B. breve, the growth rate was 0.07 h1. The authors observed inhibitory effects of B. breve in the various cultures towards the considered pathogenic bacteria. The optimal antimicrobial activity was observed in the preculture bifid with the algae culture （10 mg/mL） towards Salmonella ssp. （Zi = 25 mm）, S. aureus ATCC25923 （Zi = 26 mm） and E. coli ATCC25922 （Zi = 28 mm）.
