AIM： To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Helicobacter pylori （H pylon） as a model of Hpylori-infected gastric epithelium, and to further examine the contribut...AIM： To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Helicobacter pylori （H pylon） as a model of Hpylori-infected gastric epithelium, and to further examine the contribution of cag-pathogenicity islands （cagPAI）-coding type IV secretion system and the two pathways, nuclear factor kappa B （NF-κB） and extracellular signal-regulated kinases （ERK） on wild-type Hpylori-induced gene expression. METHODS： Gene expression profiles induced by Hpylori were evaluated in AGS gastric epithelial cells using cDNA microarray, which were present in the 4600 independent clones picked up from the human gastric tissue. We also analyzed the contribution of NF-κB and ERK signaling on H pylori-induced gene expression by using inhibitors of specific signal pathways. The isogenic mutant with disrupted cagE （△cagE） was used to elucidate the role of cagPAI-encoding type IV secretion system in the gene expression profile. RESULTS： According to the expression profile, the genes were classified into four clusters. Among them, the clusters characterized by continuous upregulation were most conspicuous, and it contained many signal transducer activity-associated genes. The role of cagPAI on cultured cells was also investigated using isogenic mutant cagE, which carries non-functional cagPAI. Then the upregulation of more than 80% of the induced genes （476/566） was found to depend on cagPAI. Signal transducer pathway through NF-κB or ERK are the major pathways which are known to be activated by cagPAI-positive H pylori. The role of these pathways in the whole signal activation by cagPAI-positive H pyloriwas analyzed. The specific inhibitors against NF-κB or ERK pathway blocked the activation of gene expression in 65% （367/566） or 76% （429/566） of the genes whose activation appealed to depend on cagPAI. CONCLUSION： These results suggest that more than half of the genes induced by cayPAI-positive H pylori depend on NF-κB and ERK signaling activation, and these pathways may play a role in the gene expression induced by hostbacterial interaction which may associate with H pylorirelated gastro-duodenal diseases.展开更多
Wireless Mesh Network has drawn much attention due to wide area service coverage with low system cost and being easy to install.However,WMN suffers from high bit error rate,which provides different link capacity among...Wireless Mesh Network has drawn much attention due to wide area service coverage with low system cost and being easy to install.However,WMN suffers from high bit error rate,which provides different link capacity among wireless mesh routers.The conventional routing metrics select the path based on link quality.The link with the best quality is preferred as the data transmission path,and thus all nodes likely select the same link,which leads to network performance degradation.This paper proposes a routing metric that considers the available bandwidth and the number of nodes suffering congestion in the path.It is confirmed that the proposed method provides higher network performance of reduced delay,reduced packet loss and increased throughput than conventional routing metrics.展开更多
In typical Wi-Fi based indoor positioning systems employing fingerprint model,plentiful fingerprints need to be trained by trained experts or technician,which extends labor costs and restricts their promotion.In this ...In typical Wi-Fi based indoor positioning systems employing fingerprint model,plentiful fingerprints need to be trained by trained experts or technician,which extends labor costs and restricts their promotion.In this paper,a novel approach based on crowd paths to solve this problem is presented,which collects and constructs automatically fingerprints database for anonymous buildings through common crowd customers.However,the accuracy degradation problem may be introduced as crowd customers are not professional trained and equipped.Therefore,we define two concepts:fixed landmark and hint landmark,to rectify the fingerprint database in the practical system,in which common corridor crossing points serve as fixed landmark and cross point among different crowd paths serve as hint landmark.Machinelearning techniques are utilized for short range approximation around fixed landmarks and fuzzy logic decision technology is applied for searching hint landmarks in crowd traces space.Besides,the particle filter algorithm is also introduced to smooth the sample points in crowd paths.We implemented the approach on off-the-shelf smartphones and evaluate the performance.Experimental results indicate that the approach can availably construct WiFi fingerprint database without reduce the localization accuracy.展开更多
Based on the spectral now and the stratification structures of the symplectic group Sp(2n, c) , the Maslov-type index theory and its generalization, the w-index theory parameterized by all ω on the unit circle, for a...Based on the spectral now and the stratification structures of the symplectic group Sp(2n, c) , the Maslov-type index theory and its generalization, the w-index theory parameterized by all ω on the unit circle, for arbitrary paths in Sp(2n, C) are established. Then the Bott-type iteration formula of the Maslov-type indices for iterated paths in Sp(2n, C) is proved, and the mean index for any path in Sp(2n, C) is defined. Also, the relation among various Maslov-type index theories is studied.展开更多
The Fibonacci numbers are the numbers defined by the linear recurrence equation, in which each subsequent number is the sum of the previous two. In this paper, we propose Fibonacci networks using Fibonacci numbers. Th...The Fibonacci numbers are the numbers defined by the linear recurrence equation, in which each subsequent number is the sum of the previous two. In this paper, we propose Fibonacci networks using Fibonacci numbers. The analyticai expressions involving degree distribution, average path lengh and mean first passage time are obtained. This kind of networks exhibits the smail-world characteristic and follows the exponential distribution. Our proposed models would provide the vaiuable insights into the deterministicaily delayed growing networks.展开更多
基金Supported by a Grant-in-Aid for Scientific Research from the Ministry of Education, Science, Sport, and Culture of Japan
文摘AIM： To elucidate the sequential gene expression profile in AGS cells co-cultured with wild-type Helicobacter pylori （H pylon） as a model of Hpylori-infected gastric epithelium, and to further examine the contribution of cag-pathogenicity islands （cagPAI）-coding type IV secretion system and the two pathways, nuclear factor kappa B （NF-κB） and extracellular signal-regulated kinases （ERK） on wild-type Hpylori-induced gene expression. METHODS： Gene expression profiles induced by Hpylori were evaluated in AGS gastric epithelial cells using cDNA microarray, which were present in the 4600 independent clones picked up from the human gastric tissue. We also analyzed the contribution of NF-κB and ERK signaling on H pylori-induced gene expression by using inhibitors of specific signal pathways. The isogenic mutant with disrupted cagE （△cagE） was used to elucidate the role of cagPAI-encoding type IV secretion system in the gene expression profile. RESULTS： According to the expression profile, the genes were classified into four clusters. Among them, the clusters characterized by continuous upregulation were most conspicuous, and it contained many signal transducer activity-associated genes. The role of cagPAI on cultured cells was also investigated using isogenic mutant cagE, which carries non-functional cagPAI. Then the upregulation of more than 80% of the induced genes （476/566） was found to depend on cagPAI. Signal transducer pathway through NF-κB or ERK are the major pathways which are known to be activated by cagPAI-positive H pylori. The role of these pathways in the whole signal activation by cagPAI-positive H pyloriwas analyzed. The specific inhibitors against NF-κB or ERK pathway blocked the activation of gene expression in 65% （367/566） or 76% （429/566） of the genes whose activation appealed to depend on cagPAI. CONCLUSION： These results suggest that more than half of the genes induced by cayPAI-positive H pylori depend on NF-κB and ERK signaling activation, and these pathways may play a role in the gene expression induced by hostbacterial interaction which may associate with H pylorirelated gastro-duodenal diseases.
基金supported by the ubiquitous Computing and Network(UCN)Projectthe Ministry of Knowledge and Econ-omy(MKE)Knowledge and Economy Frontier R&DProgramin Korea as a result of UCN′s subproject10C2-C1-20Ssupported by the MKE(The Ministry of Knowledge Economy),Korea,under the Convergence-ITRC(Convergence Infor mation Technology Research Center)support program(NIPA-2011-C6150-1101-0004)
文摘Wireless Mesh Network has drawn much attention due to wide area service coverage with low system cost and being easy to install.However,WMN suffers from high bit error rate,which provides different link capacity among wireless mesh routers.The conventional routing metrics select the path based on link quality.The link with the best quality is preferred as the data transmission path,and thus all nodes likely select the same link,which leads to network performance degradation.This paper proposes a routing metric that considers the available bandwidth and the number of nodes suffering congestion in the path.It is confirmed that the proposed method provides higher network performance of reduced delay,reduced packet loss and increased throughput than conventional routing metrics.
基金partially sponsored by National Key Project of China (No.2012ZX03001013-003)
文摘In typical Wi-Fi based indoor positioning systems employing fingerprint model,plentiful fingerprints need to be trained by trained experts or technician,which extends labor costs and restricts their promotion.In this paper,a novel approach based on crowd paths to solve this problem is presented,which collects and constructs automatically fingerprints database for anonymous buildings through common crowd customers.However,the accuracy degradation problem may be introduced as crowd customers are not professional trained and equipped.Therefore,we define two concepts:fixed landmark and hint landmark,to rectify the fingerprint database in the practical system,in which common corridor crossing points serve as fixed landmark and cross point among different crowd paths serve as hint landmark.Machinelearning techniques are utilized for short range approximation around fixed landmarks and fuzzy logic decision technology is applied for searching hint landmarks in crowd traces space.Besides,the particle filter algorithm is also introduced to smooth the sample points in crowd paths.We implemented the approach on off-the-shelf smartphones and evaluate the performance.Experimental results indicate that the approach can availably construct WiFi fingerprint database without reduce the localization accuracy.
基金National Natural Science Foundation of China MCSEC of China Qiu Shi Science and Technology Foundation.
文摘Based on the spectral now and the stratification structures of the symplectic group Sp(2n, c) , the Maslov-type index theory and its generalization, the w-index theory parameterized by all ω on the unit circle, for arbitrary paths in Sp(2n, C) are established. Then the Bott-type iteration formula of the Maslov-type indices for iterated paths in Sp(2n, C) is proved, and the mean index for any path in Sp(2n, C) is defined. Also, the relation among various Maslov-type index theories is studied.
基金Supported by the National Natural Science Foundation of China under Grant No.61203155Zhejiang Provincial Natural Science Foundation under Grant No.LQ12F03003
文摘The Fibonacci numbers are the numbers defined by the linear recurrence equation, in which each subsequent number is the sum of the previous two. In this paper, we propose Fibonacci networks using Fibonacci numbers. The analyticai expressions involving degree distribution, average path lengh and mean first passage time are obtained. This kind of networks exhibits the smail-world characteristic and follows the exponential distribution. Our proposed models would provide the vaiuable insights into the deterministicaily delayed growing networks.
