目的探究在紫外线暴露下环指蛋白20(ring finger protein 20,RNF20)低表达对胃癌细胞DNA损伤修复的影响及其相关作用机制。方法实验采用慢病毒载体构建稳定低表达胃癌细胞系,分为对照组和RNF20敲低组,用CCK-8法检测两组细胞的增殖情况,...目的探究在紫外线暴露下环指蛋白20(ring finger protein 20,RNF20)低表达对胃癌细胞DNA损伤修复的影响及其相关作用机制。方法实验采用慢病毒载体构建稳定低表达胃癌细胞系,分为对照组和RNF20敲低组,用CCK-8法检测两组细胞的增殖情况,用总共照射剂量为20 J/m^(2)紫外线照射胃癌MGC803细胞,采用Western blotting及免疫荧光技术检测两组细胞γ-H2AX、RAD51和p21的情况。结果荧光显微镜观察两组细胞均有绿色荧光蛋白表达;CCK-8显示RNF20表达降低会促进胃癌细胞增殖;敲低组细胞中RNF20蛋白较对照组表达降低。与对照组相比,经20 J/m^(2)紫外线照射细胞后,敲低组γ-H2AX消失更加迟缓,RAD51蛋白表达降低,p21蛋白表达下降趋势更慢。结论RNF20敲低会抑制紫外线诱导的胃癌细胞DNA损伤修复过程。展开更多
髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病...髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病率逐渐上升,髋臼盂唇治疗的需求得到显著的上升。随着对关节镜的运用成熟,髋关节镜技术已经成为治疗髋臼盂唇损伤的主要手术形式,包括:盂唇清理术、修复术、重建术、加强术。其主要作用都是恢复盂唇的密闭性及缓解应力分布,从而恢复髋臼功能性。因此本文主要就髋臼盂唇损伤修复进行综述。Background: The acetabular labrum is located in the hip joint and is a fibrocartilaginous ring attached to the bony acetabular rim, which is an important structure for maintaining the function of the hip joint. Damage to the labrum will change the biomechanical mechanism of the hip joint, causing joint instability and ultimately leading to the development of osteoarthritis. Due to the development of imaging technology and the popularity of sports among all people, the incidence of acetabular labral injury is gradually increasing among young people, and the demand for acetabular labral treatment has risen significantly. As the use of arthroscopy has matured, hip arthroscopy has become the main surgical form of treatment for acetabular labral injuries, including labral debridement, repair, reconstruction, and strengthening. The main purpose of these procedures is to restore labral confinement and relieve stress distribution, thus restoring acetabular function. Therefore, this article mainly reviews the repair of acetabular labral injury.展开更多
文摘髋臼盂唇位于髋关节内,是附着在骨性髋臼边缘的纤维软骨环,是维持髋关节功能的重要结构,盂唇损伤后将改变髋关节的生物力学机制,引起关节不稳,最终导致骨性关节炎的发生。因影像技术的发展及全民运动的普及,髋臼盂唇损伤在年轻人的发病率逐渐上升,髋臼盂唇治疗的需求得到显著的上升。随着对关节镜的运用成熟,髋关节镜技术已经成为治疗髋臼盂唇损伤的主要手术形式,包括:盂唇清理术、修复术、重建术、加强术。其主要作用都是恢复盂唇的密闭性及缓解应力分布,从而恢复髋臼功能性。因此本文主要就髋臼盂唇损伤修复进行综述。Background: The acetabular labrum is located in the hip joint and is a fibrocartilaginous ring attached to the bony acetabular rim, which is an important structure for maintaining the function of the hip joint. Damage to the labrum will change the biomechanical mechanism of the hip joint, causing joint instability and ultimately leading to the development of osteoarthritis. Due to the development of imaging technology and the popularity of sports among all people, the incidence of acetabular labral injury is gradually increasing among young people, and the demand for acetabular labral treatment has risen significantly. As the use of arthroscopy has matured, hip arthroscopy has become the main surgical form of treatment for acetabular labral injuries, including labral debridement, repair, reconstruction, and strengthening. The main purpose of these procedures is to restore labral confinement and relieve stress distribution, thus restoring acetabular function. Therefore, this article mainly reviews the repair of acetabular labral injury.
