A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss (A bA b) using the bacterial artificial chromosome (BAC) vector pECBAC1. The library consists of about 170 000 clones. A ...A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss (A bA b) using the bacterial artificial chromosome (BAC) vector pECBAC1. The library consists of about 170 000 clones. A random sampling analysis of 200 BAC clones indicates that the average insert size is 104 kb. Based on the genome size of T. boeoticum, the library is about three times as large as T. boeoticum haploid genome (5 600 Mb). Screening the BAC library with cpDNA sequence psbA gene and mtDNA sequence atp6 gene as probe shows that contamination of the library with chloroplast and mitochondrial clones is less than 1%. The library will be a useful platform in gene clone and genomic research of wheat.展开更多
The conceptions of theorems, laws and corollaries of hydrology were put forward. Combining with hydrology practice, several theo- rems, laws as well as corollaries of hydrology were summarized. The study provided some...The conceptions of theorems, laws and corollaries of hydrology were put forward. Combining with hydrology practice, several theo- rems, laws as well as corollaries of hydrology were summarized. The study provided some references for accelerating the development of hydrology theory in these aspects and promoting the improvement of its production technology.展开更多
文摘A bacterial artificial chromosome library has been constructed for Triticum boeoticum Boiss (A bA b) using the bacterial artificial chromosome (BAC) vector pECBAC1. The library consists of about 170 000 clones. A random sampling analysis of 200 BAC clones indicates that the average insert size is 104 kb. Based on the genome size of T. boeoticum, the library is about three times as large as T. boeoticum haploid genome (5 600 Mb). Screening the BAC library with cpDNA sequence psbA gene and mtDNA sequence atp6 gene as probe shows that contamination of the library with chloroplast and mitochondrial clones is less than 1%. The library will be a useful platform in gene clone and genomic research of wheat.
文摘The conceptions of theorems, laws and corollaries of hydrology were put forward. Combining with hydrology practice, several theo- rems, laws as well as corollaries of hydrology were summarized. The study provided some references for accelerating the development of hydrology theory in these aspects and promoting the improvement of its production technology.
