A novel outside cycle anaerobic reactor was developed based on the characteristics of expanded granular sludge bed(EGSB) and other anaerobic reactors.It was demonstrated that the sludge wash-off at high liquid upflow ...A novel outside cycle anaerobic reactor was developed based on the characteristics of expanded granular sludge bed(EGSB) and other anaerobic reactors.It was demonstrated that the sludge wash-off at high liquid upflow velocities could be controlled efficiently and good performance was achieved during municipal wastewater treatment.In order to achieve accurate prediction and effective control of sludge settling property in the reactor developed,a dispersion model was established on the basis of mass balance and free settling theory of granular sludge,taking consideration of the relevance between sludge settling velocity and sludge concentration.The simulation results of sludge distribution was in good agreement with the measured results at different liquid upflow velocities.In addition,the concentration of suspended solids(SS) in the effluent was simulated by introducing the separation efficiency of three-phase separator.The standard deviation of the simulated data was less than 6%.Based on the relative concentration of the liquid-sludge mixture,the sludge blanket height could be calculated by quadratic interpolation.The sludge blanket heights were basically identical at various upflow velocities,while the sludge concentrations at the same height differed.展开更多
目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振...目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振动应变及振动频率)(D组)4组。应用RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με(B组)作用于细胞后,细胞中OPGmRNA蛋白水平表达升高,RANKLmRNA及蛋白水平则降低(均P<0.05)。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器(振动应变2000με振动频率1.0Hz)的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。展开更多
文摘A novel outside cycle anaerobic reactor was developed based on the characteristics of expanded granular sludge bed(EGSB) and other anaerobic reactors.It was demonstrated that the sludge wash-off at high liquid upflow velocities could be controlled efficiently and good performance was achieved during municipal wastewater treatment.In order to achieve accurate prediction and effective control of sludge settling property in the reactor developed,a dispersion model was established on the basis of mass balance and free settling theory of granular sludge,taking consideration of the relevance between sludge settling velocity and sludge concentration.The simulation results of sludge distribution was in good agreement with the measured results at different liquid upflow velocities.In addition,the concentration of suspended solids(SS) in the effluent was simulated by introducing the separation efficiency of three-phase separator.The standard deviation of the simulated data was less than 6%.Based on the relative concentration of the liquid-sludge mixture,the sludge blanket height could be calculated by quadratic interpolation.The sludge blanket heights were basically identical at various upflow velocities,while the sludge concentrations at the same height differed.
文摘目的新型力学加载式生物反应器相同振动频率和不同振动应变作用于体外三维支架条件下培养的雄性大鼠成骨细胞,探究OPG/RANKL表达的影响。方法雄性大鼠成骨细胞培养、传代后分组:振动频率为1.0Hz,不同振动应变(A B C三组)及对照组(无振动应变及振动频率)(D组)4组。应用RT-PCR和Western blot检测干预后OPG和RANKL mRNA和蛋白水平的表达。结果仅振动应变2000με(B组)作用于细胞后,细胞中OPGmRNA蛋白水平表达升高,RANKLmRNA及蛋白水平则降低(均P<0.05)。其余实验组未见阳性反应。结论通过新型力学加载式生物反应器(振动应变2000με振动频率1.0Hz)的压应力作用于细胞后可以正性调节OPG/RANKL轴的表达。
