采用熔融石英毛细管,以含有50mm o l/L十二烷基硫酸钠的50mm o l/L硼酸盐缓冲液为电极缓冲液,以10mm o l/L硼酸盐缓冲液为上样缓冲液,经过对分离条件的优化,成功地建立了胶束电动毛细管色谱结合在线sw eep ing(推扫)富集技术检测中性脂...采用熔融石英毛细管,以含有50mm o l/L十二烷基硫酸钠的50mm o l/L硼酸盐缓冲液为电极缓冲液,以10mm o l/L硼酸盐缓冲液为上样缓冲液,经过对分离条件的优化,成功地建立了胶束电动毛细管色谱结合在线sw eep ing(推扫)富集技术检测中性脂溶性物质旋覆花内酯(acety lb ritann ilac tone,ABL)的实验方法。所建方法的批内、批间测定值的相对标准偏差均小于5%,灵敏度为0.005g/L,回收率大于92%;被检测样品的含量与峰面积呈良好的线性关系,相关系数为0.997 5。用所建立的方法检测了旋覆花素中ABL的含量及其在体内的动态变化,结果表明胶束电动毛细管色谱结合在线sw eep ing样品富集技术可显著提高检测的灵敏度。该方法具有操作简单、进样量小(nL级)、检测速度快等特点,弥补了毛细管电泳在测定痕量组分方面的不足。展开更多
Five new 6-O-prolinated monoacetylbritannilactones have been synthesized from N-acetylprolyl chlorides with 4-N,N-dimethylaminopyridine as catalyst and characterized by elemental analysis, IR, 1H NMR, 1H-1H COSY and M...Five new 6-O-prolinated monoacetylbritannilactones have been synthesized from N-acetylprolyl chlorides with 4-N,N-dimethylaminopyridine as catalyst and characterized by elemental analysis, IR, 1H NMR, 1H-1H COSY and MS. The compound 3b displayed a good biological activity for human HL-60 cell in vitro（ED50=4.6 mg/L）.展开更多
文摘Five new 6-O-prolinated monoacetylbritannilactones have been synthesized from N-acetylprolyl chlorides with 4-N,N-dimethylaminopyridine as catalyst and characterized by elemental analysis, IR, 1H NMR, 1H-1H COSY and MS. The compound 3b displayed a good biological activity for human HL-60 cell in vitro（ED50=4.6 mg/L）.
