In order to obtain hazelnut lines with the characteristics of strong cold re- sistance, large fruit and high quality capable of overwintering safely in Yanbian ar- eas, with 173 lines of Corylus heterophylla × C....In order to obtain hazelnut lines with the characteristics of strong cold re- sistance, large fruit and high quality capable of overwintering safely in Yanbian ar- eas, with 173 lines of Corylus heterophylla × C. heterophylla ×C. avellana hazelnut as materials, fruit-related characters were measured and analyzed. Eight excellent individuals were preliminarily selected for further identification of cold resistance and high yield. They selected single plants were 57-1, 57-3 and 55-24 of combination 6- 34-1×S1-5, 56-3, 56-5 and 55-19 of combination 6-34-1×S3-1, 21-12 of combination 6-37-2×S3-1 and 21-12 of combination 6-23-1×S3-1.展开更多
Fruit ripening is a complex process and is regulated by many factors. Ethylene and polygalacturonase (PG), lipoxygenase (LOX), expansin (EXP) are all critical regulating factors in fruit ripening and softening p...Fruit ripening is a complex process and is regulated by many factors. Ethylene and polygalacturonase (PG), lipoxygenase (LOX), expansin (EXP) are all critical regulating factors in fruit ripening and softening process. With antisense ACS tomato, Nr mutant tomato and cultivated tomato as materials, Northern blot hybridization showed that PG, LeEXP1 and LOXexpressed differently in different parts of cultivated tomato fruit during ripening, which was related to fruit ripening. The ripening process of columella and radial pericarp was faster than pericarp. In both Nr mutant and antisense ACS transgenic tomato fruit, expression levels ofPG, LeEXPI and LOXwere generally lower than those in cultivated fruit but still related to fruit ripening. The expression levels ofPG, LeEXP1 and LOX increased in the mature green tomato fruits after 0.5 h treatment with ethylene (100 μL/L). These results indicate that gene expression ofPG, LeEXP1 and LOXwere positively regulated by ethylene. The time and cumulative effect of the concentration exists in the expression of PG regulated by ethylene. The regulation of LOX expression mainly depended on the fruit development after great amount of ethylene was produced. PG played a major role in ripening and softening of tomato fruit, and cooperated with the regulation of EXP and LOX.展开更多
基金Supported by National Natural Science Foundation of China(31560548)~~
文摘In order to obtain hazelnut lines with the characteristics of strong cold re- sistance, large fruit and high quality capable of overwintering safely in Yanbian ar- eas, with 173 lines of Corylus heterophylla × C. heterophylla ×C. avellana hazelnut as materials, fruit-related characters were measured and analyzed. Eight excellent individuals were preliminarily selected for further identification of cold resistance and high yield. They selected single plants were 57-1, 57-3 and 55-24 of combination 6- 34-1×S1-5, 56-3, 56-5 and 55-19 of combination 6-34-1×S3-1, 21-12 of combination 6-37-2×S3-1 and 21-12 of combination 6-23-1×S3-1.
基金Supported by National Project of Scientific and Technical Supporting Programs Funded by Ministry of Science and Technology of China (No.2006BAD22B01)National Natural Science Foundation of China (No.30800767)Postdoctoral Fund of China (No.20080430725)
文摘Fruit ripening is a complex process and is regulated by many factors. Ethylene and polygalacturonase (PG), lipoxygenase (LOX), expansin (EXP) are all critical regulating factors in fruit ripening and softening process. With antisense ACS tomato, Nr mutant tomato and cultivated tomato as materials, Northern blot hybridization showed that PG, LeEXP1 and LOXexpressed differently in different parts of cultivated tomato fruit during ripening, which was related to fruit ripening. The ripening process of columella and radial pericarp was faster than pericarp. In both Nr mutant and antisense ACS transgenic tomato fruit, expression levels ofPG, LeEXPI and LOXwere generally lower than those in cultivated fruit but still related to fruit ripening. The expression levels ofPG, LeEXP1 and LOX increased in the mature green tomato fruits after 0.5 h treatment with ethylene (100 μL/L). These results indicate that gene expression ofPG, LeEXP1 and LOXwere positively regulated by ethylene. The time and cumulative effect of the concentration exists in the expression of PG regulated by ethylene. The regulation of LOX expression mainly depended on the fruit development after great amount of ethylene was produced. PG played a major role in ripening and softening of tomato fruit, and cooperated with the regulation of EXP and LOX.