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亚铁对杂交水稻幼苗生长和部分生理生化特性的影响 被引量:21
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作者 王贵民 陈国祥 +2 位作者 杨艳华 刘少华 邵志广 《南京师大学报(自然科学版)》 CAS CSCD 2003年第2期56-60,共5页
 通过溶液培养研究不同浓度的螯合态亚铁(EDTA Fe2+)胁迫对水稻幼苗的生长、光合速率、丙二醛和超氧阴离子的含量及抗氧化酶活性的影响.结果表明:高浓度螯合态亚铁(EDTA Fe2+)胁迫明显抑制水稻地上部分和根系的生长,水稻叶片MDA和O 2...  通过溶液培养研究不同浓度的螯合态亚铁(EDTA Fe2+)胁迫对水稻幼苗的生长、光合速率、丙二醛和超氧阴离子的含量及抗氧化酶活性的影响.结果表明:高浓度螯合态亚铁(EDTA Fe2+)胁迫明显抑制水稻地上部分和根系的生长,水稻叶片MDA和O 2的含量增高、光合速率下降.CAT,SOD在低浓度的条件下活性升高,高浓度条件下活性下降,在水稻抗铁毒害中起到一定保护作用;而POD在致死浓度条件下仍具较高的活性,在水稻抗铁毒害中起到主要保护作用. 展开更多
关键词 杂交水 稻幼苗生长 生理生化特性 螯合态亚铁 光合速率 丙二醛 超氧阴离子 抗氧化酶 抗铁毒害
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毕节地区杂交水稻推广现状及对策
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作者 谢定敏 《种子》 CSCD 北大核心 2000年第3期57-58,共2页
对毕节地区杂交水稻推广的情况进行了总结 ,肯定了取得的成绩 ,分析了存在的问题 。
关键词 杂交水 技术推广 产量 种植面积 毕节地区
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杂交水稻叶片叶龄判断方法与应用设想
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作者 黄德社 黄牧林 +2 位作者 唐启勤 舒友林 张清学 《上海农业科技》 1996年第5期12-13,共2页
自从杂交水稻问世以来,我国的广大科技工作者对如何提高杂交水稻产量和杂交水稻制种产量进行了很多研究,取得了很多成果,从而有力地推动了我国杂交水稻生产的发展。 在杂交水稻高产栽培中,根据杂交水稻主茎叶龄进程准确判断杂交水稻生育... 自从杂交水稻问世以来,我国的广大科技工作者对如何提高杂交水稻产量和杂交水稻制种产量进行了很多研究,取得了很多成果,从而有力地推动了我国杂交水稻生产的发展。 在杂交水稻高产栽培中,根据杂交水稻主茎叶龄进程准确判断杂交水稻生育期,从而采取相应的高产栽培措施,对夺取杂交水稻高产具有十分重要的作用;在杂交水稻制种实践中,要想获得制种高产,必须确保制种父、母本花期相遇。为此。 展开更多
关键词 杂交水 制种 叶片 叶龄
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两种杂交水牛乳脂肪酸组成的对比 被引量:4
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作者 李玲 农皓如 +3 位作者 唐艳 黄丽 杨攀 曾庆坤 《食品工业》 CAS 北大核心 2017年第1期266-268,共3页
对摩拉×本地、尼里-拉菲×本地两种杂交水牛乳的乳脂肪酸组成进行测定,以分析二者脂肪酸组成的差异。结果表明,摩拉×本地水牛乳脂中,短链和中链的脂肪酸、丁酸(C_(4∶0))、十四酸(C_(14∶0))和9-十六烯酸(C_(16∶1,c9))... 对摩拉×本地、尼里-拉菲×本地两种杂交水牛乳的乳脂肪酸组成进行测定,以分析二者脂肪酸组成的差异。结果表明,摩拉×本地水牛乳脂中,短链和中链的脂肪酸、丁酸(C_(4∶0))、十四酸(C_(14∶0))和9-十六烯酸(C_(16∶1,c9))显著低于尼里-拉菲×本地水牛乳(p<0.05),而长链的脂肪酸、十八酸(C_(18∶0))、二十酸(C_(20∶0))和二十二酸(C_(22∶0))含量显著高于尼里-拉菲×本地水牛乳(p<0.05),其中总长链脂肪酸(LCFA)显著高于尼里-拉菲×本地水牛乳(p<0.05)。两个品种乳脂中饱和脂肪酸(SFA)、单不饱和脂肪酸(MUFA)和多不饱和脂肪(PUFA)含量无显著差异(p>0.05)。 展开更多
关键词 杂交水牛乳 脂肪酸 摩拉 尼里-拉菲
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杂交构树叶水提液联合抗菌药对多重耐药菌的体外抑菌效果研究 被引量:5
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作者 李沁媚 徐百昌 +4 位作者 李铮 孙俊颖 陈韵如 游纯波 司红彬 《中国饲料》 北大核心 2020年第9期126-131,共6页
本文通过研究构树叶水提液与抗菌药对多重耐药菌的体外联合抑制效果,旨在为天然植物饲料添加剂的选择提供参考依据,实现畜牧业减抗替抗、发展绿色养殖的总目标。本试验通过临床收集的病料,分离出肺炎克雷伯、大肠杆菌、奇异变形杆菌、... 本文通过研究构树叶水提液与抗菌药对多重耐药菌的体外联合抑制效果,旨在为天然植物饲料添加剂的选择提供参考依据,实现畜牧业减抗替抗、发展绿色养殖的总目标。本试验通过临床收集的病料,分离出肺炎克雷伯、大肠杆菌、奇异变形杆菌、金黄色葡萄球菌4种多重耐药细菌为受试菌株,采用微量肉汤二倍稀释法,测定了构树水提液和22种抗菌药分别对四种多重耐药菌的最小抑菌浓度(MIC);采用微量棋盘法,用1/2 MIC杂交构树叶水提液与不同浓度抗菌药联合使用,测定其对该大肠杆菌的分级抑菌指数(FICI)。结果显示,杂交构树叶水提液对4种多重耐药菌有一定的抑菌效果,与部分抗菌药联用可增强抑菌效果,表明构树叶可作为饲料添加剂应用于畜牧业生产。 展开更多
关键词 杂交构树叶提液 多重耐药菌 联合使用
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浅谈杨树切枝水培杂交 被引量:1
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作者 马列明 《安徽林业》 2005年第4期26-26,共1页
杨树,自然条件下种子少而且不易采集.为选育良种,采用切枝水培杂交技术,便于调整花期,保证父本的纯正,为杨树家系育种奠定良好基础.
关键词 杨树 切枝杂交 雌雄株辩别 采枝时间 培管理 花粉
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水旱稻三交种的应用价值初步研究 被引量:6
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作者 张安宁 王飞名 +4 位作者 余新桥 李明寿 梁文兵 梅捍卫 罗利军 《中国水稻科学》 CAS CSCD 北大核心 2009年第3期327-330,共4页
以水稻野败不育系ZZA、旱稻野败不育系保持系沪旱1B和旱稻恢复系旱恢3号配制的一个水旱稻三交种(ZZA/沪旱1B//旱恢3号)和两个相应的单交种(ZZA/旱恢3号和沪旱1A/旱恢3号)为材料,进行田间性状调查,测定产量、抗旱性和稻米品质等指标。结... 以水稻野败不育系ZZA、旱稻野败不育系保持系沪旱1B和旱稻恢复系旱恢3号配制的一个水旱稻三交种(ZZA/沪旱1B//旱恢3号)和两个相应的单交种(ZZA/旱恢3号和沪旱1A/旱恢3号)为材料,进行田间性状调查,测定产量、抗旱性和稻米品质等指标。结果表明,三交种株高、叶绿素含量、穗长等性状与单交种的变异系数差异不显著,但群体抽穗历期延长;产量明显高于单交种,2006年比两单交种分别增产17.33%和25.63%,2007年比两单交种分别增产3.57%和11.71%;抗旱性增强,抗旱系数达51.21%,高于两单交种和亲本;主要稻米品质指标与两单交种差异不大。结果表明,水旱稻三交种可以拓展杂交稻的遗传基础,具有一定的应用价值。 展开更多
关键词 三交种 单交种 旱稻杂交 旱稻 抗旱性
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农业生物技术的现状与发展方向 被引量:1
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作者 彭明 《世界热带农业信息》 2005年第11期1-3,共3页
关键词 农业生物技术 生物技术产业 稻基因组 技术产业化 棉花品质 体细胞克隆 植物生物反应器 超级杂交水
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Genetic and Correlation Analysis of Grain Characters in Three-line Hybrid Rice 被引量:11
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作者 邹小云 邓辉明 +6 位作者 傅军如 彭小松 朱昌兰 贺晓鹏 陈小荣 贺浩华 刘宜柏 《Agricultural Science & Technology》 CAS 2009年第2期77-81,共5页
Abst[Objective] This study was to understand the genetic dynamics of three-line hybrid rice, and explore the respective effect of sterile line and restoring line on grain characters of hybrid rica. [Method] Four three... Abst[Objective] This study was to understand the genetic dynamics of three-line hybrid rice, and explore the respective effect of sterile line and restoring line on grain characters of hybrid rica. [Method] Four three-line stedle lines and 27 restoring lines(cultivars) commonly culti- vated in Central China region were regarded as expadmental materials to conduct 4 x27NCII cross design, and the grain characters of three-line hybrid rico were analyzed at genetic and correlation levels. [ Result] Four characters of grain length, grain width, 1 000-grain weight and length- width ratio play the leading role in additive gene effect; these four characters were simultaneously influenced by male parent and female parent, but the effect from male parent was relatively larger. The grain length, grain width, 1 000-grain weight and length-width ratio all have high brood hedtabUities( respectively 99.65%, 98.31%, 95.27% and 98.81% ). Correlation analysis showed that grain length was positively correlated with 1 000-grain weight and length-width ratio at extremely significant level; 1 000-grain weight was positively correlated with grain length and length- width ratio at extremely significant level, and was insignificantly correlated with grain width; grain width was negatively correlated with grain length and length-width ratio at extremely significant level. Path analysis showed that the direct path coefficients of grain length, grain width and 1 0(30- grain weight to length-width ratio were 0.624 6, -0.555 9 and -0.015 8, respectively. [ Conclusion] This study systematically analyzed the effects of stedle line and restoring line on grain characters of hybrid rice, which provided theoretical basis for breeding high quality and yield hy- brid dce. 展开更多
关键词 Three-line hybrid dce Grain characters INHERITANCE . Correlation
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Isolation and Identification of Submergence-induced Genes in Maize (Zea mays) Seedlings by Suppression Subtractive Hybridization 被引量:12
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作者 高鹏 王国英 +2 位作者 赵虎基 樊立 陶亚忠 《Acta Botanica Sinica》 CSCD 2003年第4期479-483,共5页
To investigate the expression profile of maize genes induced by submergence, a subtracted cDNA library of maize seedling roots was constructed using suppression subtractive hybridization (SSH). The cDNA of maize seedl... To investigate the expression profile of maize genes induced by submergence, a subtracted cDNA library of maize seedling roots was constructed using suppression subtractive hybridization (SSH). The cDNA of maize seedling roots treated with submergence (ST) was used as tester and what from untreated roots (UT) as driver. Products of the secondary PCR from the forward subtraction were cloned into T/A vector and transferred into Escherichia coli strain JM10B by electroporation. Four hundred and eight randomly chosen transformants carrying cDNA fragments were screened with PCR-Select Deferential Screening Kit. One hundred and eighty-four cDNA clones were identified as, submergence specifically induced or highly expressed. After sequencing and removing redundant cDNAs, we got 95 submergence-induced cDNA clones. Of the 95 cDNA clones, 68 contain the regions with 60%-90% identity to their homolog in GenBank, 21 are expected to be novel genes, only 6 correspond to the published maize sequences. 展开更多
关键词 MAIZE expression profile suppression subtractive hybridization (SSH) SUBMERGENCE
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Study on Mechanization Hybrid Rice Seed Production Technology and Combine Breeding Advances 被引量:5
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作者 汪婉琳 朱启升 +2 位作者 王士梅 杨前进 张德文 《Agricultural Science & Technology》 CAS 2011年第1期90-92,共3页
[Objectives]Current hybrid rice seed production mechanization and a new kind of hybrid rice seed production mechanization using Bentazone sensitive lethal genes for mechanized seed production technology are introduced... [Objectives]Current hybrid rice seed production mechanization and a new kind of hybrid rice seed production mechanization using Bentazone sensitive lethal genes for mechanized seed production technology are introduced in this paper.[Method]Sensitive Restorer Bentazone lethal dose of filtering,parental and seed weight are bred combinations of several aspects of the system described,and presented mechanized seed technology new research directions.[Results]The method of using Bentazone sensitive lethal genes for mechanized seed production technology is feasible,economic,seed production and combination advantages.[Conclusions] Using Bentazone sensitive lethal genes for mechanized seed production technology is the new study direction of mechanic production. 展开更多
关键词 Hybrid rice Mechanization seed production techniques BENTAZONE-SENSITIVITY-GENE
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Solutions to Insecurity Problems in Seed Production of Two-line Hybrid Rice 被引量:2
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作者 雷东阳 唐文帮 +2 位作者 解志坚 刘海 陈立云 《Agricultural Science & Technology》 CAS 2014年第7期1160-1166,1187,共8页
The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situat... The insecure problems of seed production have seriously hampered the healthy and sustainable development of two-line hybrid rice.The safety issues on seed production of two-line hybrid rice and current research situation were pointed out in this paper.The three main reasons for unsafety in seed production of twoline hybrid rice were unsuitable site selection,high critical sterility-inducing temperature and the drift of critical temperature.In this paper,strategies and measures were put forward based on many years of practice.It could minimize the risk in seed production of two-line hybrid rice by selecting dual-purpose genic male sterile line with lower critical sterility-inducing temperature and long lower temperature resistant time.Based on the climate data and climatic demands of the "three safe-periods" in seed production,a new idea for determining appropriate bases and periods for seed production was proposed by using computer technology,which solved the aimless selection of sites and periods for the seed production of two-line hybrid rice.Besides,we established a system of single plant selection and identification method and original seed propagation with cyclic cold water,which could reduce the generation number of original seed propagation in seed production and avoid the drift of critical sterility-inducing temperature.This paper improved the seed production safety in the three aspects of seed nature,seed source and seed production site. 展开更多
关键词 Two-line hybrid rice Dual-purpose genic male sterile line Safety of seed production
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The Application of Bentazon Susceptible Gene on Seed Production of Hybrid Rice 被引量:1
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作者 王士梅 朱启升 +3 位作者 汪婉琳 张德文 杨前进 王云生 《Agricultural Science & Technology》 CAS 2008年第1期99-103,145,共6页
The seed production of male and female parent of ‘Hunzhi 1' contained bentazon susceptible gene was studied. The result showed that spraying 3. 375 L/hm2 48% bentazon aqua could control the self-pollinated fertiliza... The seed production of male and female parent of ‘Hunzhi 1' contained bentazon susceptible gene was studied. The result showed that spraying 3. 375 L/hm2 48% bentazon aqua could control the self-pollinated fertilization rate below 1.0% and survival plant rate was 0. The spray of bentazon would not influence MC526 pollen vitality. Seed setting rate of female parent was not obvious different from that of control group with spraying bentazon aqua more than 3. 375 L/hm^2 from the sprouting appearing period to full heading stage. This demonstrated that bentazon aqua had no influence on seed setting rate during florescence of female parent. The weight ratio between male parent seeds and female parent seeds was 1 to 5, then seeds from parents with that weight ratio would be mixedly planted for seed production. With this weight ratio, relative proper structure of panicle and grain would be obtained ,panicle ratio was 1 to 4.3, glumous flowers ratio was 1 to 3.5. Cross seed-setting rate of female parent was 72.6% and seed production per hm^2 was 66 000 kg. 展开更多
关键词 Hybrid rice BENTAZON Susceptible restorer Technique of seed production
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Effects of Nitrogen Quantity on Grain-filling Characters of Two-line Hybrid Rice with Large Ear 被引量:1
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作者 刘洪 李之林 +3 位作者 徐振江 王维 任永浩 肖立中 《Agricultural Science & Technology》 CAS 2012年第8期1621-1624,1674,共5页
[Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made us... [Objective] The aim was to research effects of N quantity on grain-filling characters of two-line hybrid rice cultivars with large ears. [Method] Peiza 67 and 88, two-line hybridized rice with large ears, were made use of to study on effects of N fertilizer in different quantities (LN: 90 kg/hm2;MN: 180 kg/hm2;HN: 270 kg/hm2) on plumpness and grain-filling characters. [Result] When N fertilizers were excessive, for inferior grains, grain-filling rate decreased and grain-filling time extended, resulting in plumpness decline after degradation of leaves' function. When N fertilizers were inadequate, maximal and average grain-filling rates decreased and the differences between superior and inferior grains in grain-filling rate increased, leading to decline of grain's weight and plumpness degree. On the other hand, quantity of N fertilizers had little effect on superior grains in plumpness. [Conclusion] The research provided references for reasonable use of N fertilizer and improvement of rice yield and N use. 展开更多
关键词 Nitrogen rate Two-line hybrid rice SEEDS Characters of grain filling
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Great progress in molecular breeding of hybrid rice in CNHRRDC 被引量:3
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作者 赵炳然 袁隆平 《Agricultural Science & Technology》 CAS 2005年第3期2-6,共5页
Elite maintainer lines and restorer lines have been developed by genomic DNA transformation,Analyses of molecular markers, DNA sequences, and Southern blotting have revealed that high DNA polymorphism exists between n... Elite maintainer lines and restorer lines have been developed by genomic DNA transformation,Analyses of molecular markers, DNA sequences, and Southern blotting have revealed that high DNA polymorphism exists between new developed lines and its receptors, indicating that the special DNA fragment from distant relatives may be integrated into the genome of rice. And several combinations with the potential of super-high yield have been developed from these lines. Therefore, transformation of genomic DNA from distant relatives to the plant of a target receptor may open an avenue for breeding of super-hybrid rice. 展开更多
关键词 Super-hybrid rice molecular breeding genomic DNA transformation GERMPLASM
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Isolation of Zizania latifolia Species-specific DNA Sequences and Their Utility in Identification of Z. latifolia DNA Introgressed into Rice 被引量:5
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作者 刘振兰 董玉柱 刘宝 《Acta Botanica Sinica》 CSCD 2000年第3期324-326,共3页
根据两个植物抗病基因N和RPS2中核酸结合位点 (NBS)和富亮氨酸重复区 (LRR)中的保守序列设计了一对特异引物 ,用PCR从具有水稻 (OryzasativaL .)改良所需要的许多优良性状的水稻近缘野生种菰 (Zizanialatifolia(Griseb .)Turcz.exStapf... 根据两个植物抗病基因N和RPS2中核酸结合位点 (NBS)和富亮氨酸重复区 (LRR)中的保守序列设计了一对特异引物 ,用PCR从具有水稻 (OryzasativaL .)改良所需要的许多优良性状的水稻近缘野生种菰 (Zizanialatifolia(Griseb .)Turcz.exStapf)的基因组DNA中扩增同源片段。PCR产物经克隆后 ,分别以菰和水稻的基因组DNA为探针 ,通过点杂交对所得克隆进行了分析。点杂交结果表明 ,在所分析的 6 0个克隆中有 2个克隆是菰专化的序列 ,即它们与水稻无杂交信号。基因组DNA的Southern杂交进一步证实了这 2个克隆的专化性。为了验证一些可能的“水稻_菰”渐渗杂交系是否确实含有源自供体菰的DNA ,以这 2个克隆为探针 ,与经EcoRⅠ酶切的 5个可能的渐渗杂交系进行了Southern杂交。结果表明 ,这 2个克隆均能检测出其中的一个系含有其同源序列。这一结果为曾经报道的经一种非常规有性杂交方法将菰DNA导入水稻提供了确凿的证据。 展开更多
关键词 species_specific DNA sequence introgression lines Zizania latifolia RICE
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Study on the Agroclimatic Division of Thermosensitive Genetic Male Sterility Rice Breeding 被引量:1
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作者 陈小敏 陈汇林 +1 位作者 邹海平 王斌 《Agricultural Science & Technology》 CAS 2015年第2期350-354,390,共6页
Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was m... Using the meteorological data and geographical information during January-March(1961-2010) accumulated by 18 stations of Hainan,a suitability zoning map for thermo-sensitive genetic male sterility(TGMS) rice was made by GIS technology based on temperature indicators required by TGMS rice during fertility sensitive period and heading-flowering period,aiming to provide reasonable layout and scientific basis for sustainable development of TGMS rice in Hainan Island under the background of global warming.The results indicated that the suitable planting zones covered the south regions of Wuzhishan,Jianfengling and Diaoluoshan;subordinate suitable zones expanded northward to central parts of Wuzhishan,Dongfang,Changjiang,Qiongzhong and southern parts of Wanning;the other regions were unsuitable for breeding. 展开更多
关键词 Thermo-sensitive genetic male sterility(TGMS) rice Fertility sensitive period Heading-flowering period Agroclimatic division HAINAN
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Research Progress on Mechanized Mixed Sowing Seed Production Technology of Hybrid Rice 被引量:4
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作者 张德文 《Agricultural Science & Technology》 CAS 2017年第5期924-929,939,共7页
Hybrid rice planting has been widely popularized and applied in the world. However, the high cost of seed production and the complicated procedures have become a bottleneck in the development of hybrid rice. The resea... Hybrid rice planting has been widely popularized and applied in the world. However, the high cost of seed production and the complicated procedures have become a bottleneck in the development of hybrid rice. The research progress on mixed sowing seed production techniques of hybrid rice was introduced from the aspects of rice resources creation, breeding, sowing seed technology research and cost benefit analysis. The production technology of the new mixed seeding combina- tion "Xinhunyou 6" was investigated, including the research and validation of benta- zon treatment period and dosage, mixing ratio of male and female parents, and the comparative test of different different sowing methods, which revealed that the mechanization technology of seed production of hybrid rice was mature and feasible and would be one of the most important development trend of technological devel- opment of hybrid rice production. 展开更多
关键词 Hybrid rice Mechanized seed production Glume color marker gene BENTAZON Research progress
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Effect of Microbial Inoculum Fertilizer on the Growth and Yield of Super Hybrid Rice 被引量:1
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作者 郭夏宇 艾治勇 《Agricultural Science & Technology》 CAS 2015年第11期2396-2398,共3页
The research designed four application quantities of 0, 2 250, 4 500 and 6 750 g/hm2 based on Liangyou No. 959 to explore the effects of microbial inocu- lure fertilizer on growth and yield of super hybrid rice. The r... The research designed four application quantities of 0, 2 250, 4 500 and 6 750 g/hm2 based on Liangyou No. 959 to explore the effects of microbial inocu- lure fertilizer on growth and yield of super hybrid rice. The results indicate that the treatment with traditional fertilizers and 4 500 g/hm2 HYT microbial inoculum fertilizer performed the best, where average number of tiller was 2.4 more than that of the control; the number of effective ear was 11.05% higher; yield increased by 10.3%. These have demonstrated that microbial inoculum fertilizer is of great value in pro- motion on super hybrid rice production. 展开更多
关键词 Microbial inoculum fertilizer Super hybrid rice Growth and development Yieid
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Analysis of High Yield and Efficiency Technique in Hybrid Rice Zhongzheyou No.1 被引量:6
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作者 刘伟明 《Agricultural Science & Technology》 CAS 2009年第2期73-76,96,共5页
To understand the high yield and efficiency technique in hybrid rice Zhongzheyou No. 1, we conducted the correlation analysis, regression analysis and path analysis of hybrid rice Zhongzheyou No. 1 based on the data o... To understand the high yield and efficiency technique in hybrid rice Zhongzheyou No. 1, we conducted the correlation analysis, regression analysis and path analysis of hybrid rice Zhongzheyou No. 1 based on the data of its ear, grain and weight at different yield levels. From this study, we put forward the high yield and efficiency technique in Zhongzheyou No. 1 .. on the basis of certain effective ear number, filled grains per ear should be mainly targeted with a consideration to 1 000-grain weight. 展开更多
关键词 Zhongzheyou No. 1 Single-cropping dce High Yield and Efficiency Technique pathway
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