The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramib...The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramibalis c.v and Populus deltoids） suffocated by Methyl jasmonate （MeJA） and Methyl salicylate （MeSA） were monitored for analyzing their functions in interplant communications by using high-pressure liquid chromatography （HPLC）.The results showed that the contents of phenolic acids had obviously difference in leaves exposed to either MeSA or MeJA.When P.deltoides leaves exposed to MeJA or MeSA, the level of gallic acid, coumaric acid, caffeic acid, ferulic acid and benzoic acid was increased, gallic acid in leaves treated with MeJA comes to a peak at 24 h while to a peak at 12-d having leaves treated with MeSA.When P.Simonii ×P.Pyramibalis c.v leaves were exposed to MeJA or MeSA, the level of gallic acid, pyrocatechol and ferulic acid was increased; The catechinic acid and benzoic acid had a little drop; The caffeic acid and coumaric acid were undetected in both suffocated and control leaves.This changed pattern indicated that MeJA and MeSA can act as airborne signals to induce defense response of plants.展开更多
[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[...[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[Method]Using T1 seedlings of sm-Ngt transgenic tobacco lines containing Gus gene controlled by five 5＇ flank deletion promoter fragments different in length as experimental materials,GUS histochemical staining and fluorometric analysis of T1 seedlings treated with MeJA and SA for 16 h were conducted to analyze the effect of MeJA and SA treatment on the expression of 5＇ flank deletion promoter fragments.[Result]Of five 5＇ flank deletion promoter fragments transgenic plant lines,30 d old T1 seedlings containing 220-0 bp promoter fragment performed worst in GUS staining（showing least staining spots）,those containing-524-0 bp and-468-0 bp promoter fragment both performed best.In the plants not treated with MeJA and SA,activities of GUS driven by-524-0 bp and-468-0 bp deletion promoter fragments were enormously higher than that driven by-1 150-0,-800-0 or-220 0 bp,and which were proved to be not resulted from insert copy number by Southern blot.For GUS expression,promoter fragment-800-0 bp expression was doubly induced by both MeJA and SA,while fragment-1 150-0 was induced by MeJA.[Conclusion]There are activity enhancement elements within-524--220 bp of the sm-Ngt in promoter and activity down regulation elements within-1 150--524 bp region,as well as MeJA and SA doubly inducing activity regulation elements in this promoter.展开更多
Glucosinolates （GSs） are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid （JA） and salicylic acid （SA） are major regulators of plant response to pathogen ...Glucosinolates （GSs） are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid （JA） and salicylic acid （SA） are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage （Brassica rapa ssp. pekinensis）. In this study, we applied different concentrations of methyl jasmonate （MeJA） and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Re- gardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS （IGS） were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that ac- cumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin （neoGBC） did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin （GBC） to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin （4-MGBC） occurred only in the root irrespective of the type of elicitors and the site of elici- tation. Thus, accumulation of IGS is a major metabolic hallmark of SA＊ and MeJA-mediated systemic response sys- tems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.展开更多
基金This research is supported by National Natural Science Foundation of China (No.30170764)
文摘The contents of seven different phenolic acids such as gallic acid, catechinic acid, pyrocatechol, caffeic acid, coumaric acid, ferulic acid and benzoic acid in the poplar leaves （Populus Simonii×Populus Pyramibalis c.v and Populus deltoids） suffocated by Methyl jasmonate （MeJA） and Methyl salicylate （MeSA） were monitored for analyzing their functions in interplant communications by using high-pressure liquid chromatography （HPLC）.The results showed that the contents of phenolic acids had obviously difference in leaves exposed to either MeSA or MeJA.When P.deltoides leaves exposed to MeJA or MeSA, the level of gallic acid, coumaric acid, caffeic acid, ferulic acid and benzoic acid was increased, gallic acid in leaves treated with MeJA comes to a peak at 24 h while to a peak at 12-d having leaves treated with MeSA.When P.Simonii ×P.Pyramibalis c.v leaves were exposed to MeJA or MeSA, the level of gallic acid, pyrocatechol and ferulic acid was increased; The catechinic acid and benzoic acid had a little drop; The caffeic acid and coumaric acid were undetected in both suffocated and control leaves.This changed pattern indicated that MeJA and MeSA can act as airborne signals to induce defense response of plants.
基金Supported by Natural Science Foundation of Hubei Province(2004ABA123)~~
文摘[Objective]The study was to analyze the expression of the deletion fragments from the promoter of a glycosyltransferase gene induced both by MeJA and SA cloned from tobacco W38（sm-Ngt） in transgenic tobacco plants.[Method]Using T1 seedlings of sm-Ngt transgenic tobacco lines containing Gus gene controlled by five 5＇ flank deletion promoter fragments different in length as experimental materials,GUS histochemical staining and fluorometric analysis of T1 seedlings treated with MeJA and SA for 16 h were conducted to analyze the effect of MeJA and SA treatment on the expression of 5＇ flank deletion promoter fragments.[Result]Of five 5＇ flank deletion promoter fragments transgenic plant lines,30 d old T1 seedlings containing 220-0 bp promoter fragment performed worst in GUS staining（showing least staining spots）,those containing-524-0 bp and-468-0 bp promoter fragment both performed best.In the plants not treated with MeJA and SA,activities of GUS driven by-524-0 bp and-468-0 bp deletion promoter fragments were enormously higher than that driven by-1 150-0,-800-0 or-220 0 bp,and which were proved to be not resulted from insert copy number by Southern blot.For GUS expression,promoter fragment-800-0 bp expression was doubly induced by both MeJA and SA,while fragment-1 150-0 was induced by MeJA.[Conclusion]There are activity enhancement elements within-524--220 bp of the sm-Ngt in promoter and activity down regulation elements within-1 150--524 bp region,as well as MeJA and SA doubly inducing activity regulation elements in this promoter.
基金supported by the National Natural Science Foundation of China(Nos.31000916 and 30871718)the Zhejiang Provincial Natural Science Foundation of China(Nos.LY14C150005,LZ14C150001,and Y3090538)+3 种基金the Scientific Research Starting Foundation for the Returned Overseas Chinese Scholars from the Ministry of Education of Chinathe Climbing Program for Young Academic Leaders in Universities of Zhejiang Province(No.pd2013230)the Qianjiang Talent Project of Zhejiang Province(No.qjd0902010)the Key Sci-Technology Project of Zhejiang Province(No.2010C12004),China
文摘Glucosinolates （GSs） are an important group of defensive phytochemicals mainly found in Brassicaceae. Plant hormones jasmonic acid （JA） and salicylic acid （SA） are major regulators of plant response to pathogen attack. However, there is little information about the interactive effect of both elicitors on inducing GS biosynthesis in Chinese cabbage （Brassica rapa ssp. pekinensis）. In this study, we applied different concentrations of methyl jasmonate （MeJA） and/or SA onto the leaf and root of Chinese cabbage to investigate the time-course interactive profiles of GSs. Re- gardless of the site of the elicitation and the concentrations of the elicitors, the roots accumulated much more GSs and were more sensitive and more rapidly responsive to the elicitors than leaves. Irrespective of the elicitation site, MeJA had a greater inducing and longer lasting effect on GS accumulation than SA. All three components of indole GS （IGS） were detected along with aliphatic and aromatic GSs. However, IGS was a major component of total GSs that ac- cumulated rapidly in both root and leaf tissues in response to MeJA and SA elicitation. Neoglucobrassicin （neoGBC） did not respond to SA but to MeJA in leaf tissue, while it responded to both SA and MeJA in root tissue. Conversion of glucobrassicin （GBC） to neoGBC occurred at a steady rate over 3 d of elicitation. Increased accumulation of 4-methoxy glucobrassicin （4-MGBC） occurred only in the root irrespective of the type of elicitors and the site of elici- tation. Thus, accumulation of IGS is a major metabolic hallmark of SA＊ and MeJA-mediated systemic response sys- tems. SA exerted an antagonistic effect on the MeJA-induced root GSs irrespective of the site of elicitation. However, SA showed synergistic and antagonistic effects on the MeJA-induced leaf GSs when roots and leaves are elicitated for 3 d, respectively.
