甲状腺滤泡性癌(FTC)和滤泡性腺瘤(FTA)需组织病理仔细鉴别,前者的预后主要基于肿瘤大小和侵袭范围,而高危病例缺乏精确的免疫组化标记物。作者试图用Ki-67来区分FTC和FTA并识别预后不良的FTC,为此收集了所在单位1999年以来818例甲状腺...甲状腺滤泡性癌(FTC)和滤泡性腺瘤(FTA)需组织病理仔细鉴别,前者的预后主要基于肿瘤大小和侵袭范围,而高危病例缺乏精确的免疫组化标记物。作者试图用Ki-67来区分FTC和FTA并识别预后不良的FTC,为此收集了所在单位1999年以来818例甲状腺滤泡性肿瘤,其中包括516例FTA、252例FTC、50例恶性潜能未定的滤泡性肿瘤(FT-UMP)。研究仅包括真性无功能的滤泡性肿瘤,而毒性腺瘤、玻璃样梁状肿瘤、低分化成分和/或乳头状甲状腺癌与髓样癌共存,以及恶性潜能未定的高分化肿瘤(WDT-UMPs)均被排除在外。结果显示,嗜酸细胞肿瘤或“Hürthle细胞”滤泡性甲状腺肿瘤共75例。FTC通常体积大于FTA(41 mm vs.31 mm,P<0.001),FTC患者平均年龄比FTA的大(55岁vs.50岁,P<0.001),而FTC和FTUMP之间无显著差异。在分析了患者手术年龄、性别、原发肿瘤大小、Ki-67增殖指数、有无甲状腺外播散(ETE)、包膜侵犯、血管侵犯、嗜酸细胞分化及pTNM分期各个参数后,发现手术年龄、Ki-67增殖指数和ETE的存在是转移/复发性疾病(无病生存)独立预测因子,而Ki-67增殖指数和手术时年龄是预测患者死于疾病的独立因素。血管浸润被定义为血管内瘤栓外层与纤维蛋白相连或轮廓超出肿瘤包膜,若判定困难,可水平切片、van Gieson染色和/或行内皮免疫组化标记。展开更多
目的:探讨应用鸡尾酒双染法双标Ki-67和LCA(白细胞共同抗原,leukocyte common antigen,CD45)以提高脑膜瘤中Ki-67标记指数准确性的研究。方法收集郑州大学第一附属医院病理科2014年1月-2014年9月确诊的良性脑膜瘤组织标本29例。每例组...目的:探讨应用鸡尾酒双染法双标Ki-67和LCA(白细胞共同抗原,leukocyte common antigen,CD45)以提高脑膜瘤中Ki-67标记指数准确性的研究。方法收集郑州大学第一附属医院病理科2014年1月-2014年9月确诊的良性脑膜瘤组织标本29例。每例组织分别进行LCA/Ki-67鸡尾酒双染,并同时做Ki-67和LCA两种抗体的单染。显微镜下得出Ki-67标记指数,并进行统计学分析。结果单染组Ki-67标记指数平均值高于鸡尾酒双染组的Ki-67标记指数,差异具有统计学意义(p<0.05)。结论应用LCA和Ki-67鸡尾酒双染法是提高脑膜瘤中肿瘤标记Ki-67计数准确性的有效方法,具备一定的临床推广价值。展开更多
Purpose: To evaluate the relationships among histopathological type, clinical malignant grade, and Ki-67 labeling index (LI) in sebaceous gland carcinoma (SGC), conjunctival squamous cell carcinoma (SCC), and conjunct...Purpose: To evaluate the relationships among histopathological type, clinical malignant grade, and Ki-67 labeling index (LI) in sebaceous gland carcinoma (SGC), conjunctival squamous cell carcinoma (SCC), and conjunctival intraepithelial neoplasia (CIN), with pterygium and normal conjunctiva as controls. Methods: This retrospective study was conducted at the Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. We used tissue specimens obtained from 20 patients (four SGC, four SCC, four CIN, four pterygium, and four normal conjunctiva). Ki-67 immunohistochemical analysis was performed in all 20 cases. Results: The Ki-67 labeling index (LI) was 46.1± 3.0% (average± SD) in SGC, 28.4± 4.5% in SCC, 20.0± 7.2% in CIN, 9.0± 2.2% in pterygium, and 6.8± 2.3% in normal conjunctiva. Ki-67 LI was significantly (Mann-Whitney U test, P < 0.05) higher in SGC than in SCC, and higher, but not significantly, in SCC than in CIN. Ki-67 LI was significantly (P < 0.05) higher in SCC and CIN than in pterygium. Conclusions: These results suggest that Ki-67 LI may be a sensitive marker for ocular malignant tumor grading.展开更多
文摘甲状腺滤泡性癌(FTC)和滤泡性腺瘤(FTA)需组织病理仔细鉴别,前者的预后主要基于肿瘤大小和侵袭范围,而高危病例缺乏精确的免疫组化标记物。作者试图用Ki-67来区分FTC和FTA并识别预后不良的FTC,为此收集了所在单位1999年以来818例甲状腺滤泡性肿瘤,其中包括516例FTA、252例FTC、50例恶性潜能未定的滤泡性肿瘤(FT-UMP)。研究仅包括真性无功能的滤泡性肿瘤,而毒性腺瘤、玻璃样梁状肿瘤、低分化成分和/或乳头状甲状腺癌与髓样癌共存,以及恶性潜能未定的高分化肿瘤(WDT-UMPs)均被排除在外。结果显示,嗜酸细胞肿瘤或“Hürthle细胞”滤泡性甲状腺肿瘤共75例。FTC通常体积大于FTA(41 mm vs.31 mm,P<0.001),FTC患者平均年龄比FTA的大(55岁vs.50岁,P<0.001),而FTC和FTUMP之间无显著差异。在分析了患者手术年龄、性别、原发肿瘤大小、Ki-67增殖指数、有无甲状腺外播散(ETE)、包膜侵犯、血管侵犯、嗜酸细胞分化及pTNM分期各个参数后,发现手术年龄、Ki-67增殖指数和ETE的存在是转移/复发性疾病(无病生存)独立预测因子,而Ki-67增殖指数和手术时年龄是预测患者死于疾病的独立因素。血管浸润被定义为血管内瘤栓外层与纤维蛋白相连或轮廓超出肿瘤包膜,若判定困难,可水平切片、van Gieson染色和/或行内皮免疫组化标记。
文摘目的:探讨应用鸡尾酒双染法双标Ki-67和LCA(白细胞共同抗原,leukocyte common antigen,CD45)以提高脑膜瘤中Ki-67标记指数准确性的研究。方法收集郑州大学第一附属医院病理科2014年1月-2014年9月确诊的良性脑膜瘤组织标本29例。每例组织分别进行LCA/Ki-67鸡尾酒双染,并同时做Ki-67和LCA两种抗体的单染。显微镜下得出Ki-67标记指数,并进行统计学分析。结果单染组Ki-67标记指数平均值高于鸡尾酒双染组的Ki-67标记指数,差异具有统计学意义(p<0.05)。结论应用LCA和Ki-67鸡尾酒双染法是提高脑膜瘤中肿瘤标记Ki-67计数准确性的有效方法,具备一定的临床推广价值。
文摘Purpose: To evaluate the relationships among histopathological type, clinical malignant grade, and Ki-67 labeling index (LI) in sebaceous gland carcinoma (SGC), conjunctival squamous cell carcinoma (SCC), and conjunctival intraepithelial neoplasia (CIN), with pterygium and normal conjunctiva as controls. Methods: This retrospective study was conducted at the Department of Ophthalmology, Kyoto Prefectural University of Medicine, Kyoto, Japan. We used tissue specimens obtained from 20 patients (four SGC, four SCC, four CIN, four pterygium, and four normal conjunctiva). Ki-67 immunohistochemical analysis was performed in all 20 cases. Results: The Ki-67 labeling index (LI) was 46.1± 3.0% (average± SD) in SGC, 28.4± 4.5% in SCC, 20.0± 7.2% in CIN, 9.0± 2.2% in pterygium, and 6.8± 2.3% in normal conjunctiva. Ki-67 LI was significantly (Mann-Whitney U test, P < 0.05) higher in SGC than in SCC, and higher, but not significantly, in SCC than in CIN. Ki-67 LI was significantly (P < 0.05) higher in SCC and CIN than in pterygium. Conclusions: These results suggest that Ki-67 LI may be a sensitive marker for ocular malignant tumor grading.
